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References
Anandarajah, K. & B.D. McKersie, 1990. Manipulating the desiccation tolerance and vigor of dry somatic embryos of Medicago sativa L. with sucrose, heat shock and abscisic acid. Plant Cell Rep. 9: 451–455.
Bomal, C., V.Q. Le & F.M. Tremblay, 2002. Induction of tolerance to fast desiccation in black spruce (Picea mariana) somatic embryos: relationship between partial water loss, sugars and dehydrins. Physiol. Plant. 115: 423–530.
Bomal, C. & F.M. Tremblay, 1999. Effect of desiccation to low moisture content on germination, synchronization of root emergence, and plantlet regeneration of black spruce somatic embryos. Plant Cell Tiss. Org. Cult. 56:193–200
Bomal, C. & F.M. Tremblay, 2000. Dried cryopreserved somatic embryos of two Picea species provide suitable material for direct plantlet regeneration and germplasm storage. Ann. Bot. 86: 177–183.
Campbell, R.A. & D.J. Durzan, 1975. Induction of multiple buds and needles in tissue cultures of Picea glauca. Can. J. Bot. 53: 1652–1657.
El Meskaoui, A., Y. Desjardins & F. M. Tremblay, 2000. Kinetics of ethylene biosynthesis and its effects during maturation of white spruce somatic embryos. Physiol. Plant. 109: 333–342.
El Meskaoui, A. & F.M. Tremblay, 1999. Effects of sealed and vented gaseous microenvironments on the maturation of somatic embryos of black spruce with a special emphasis on ethylene. Plant Cell Tiss. Org. Cult. 56: 201–209.
El Meskaoui, A. & F.M. Tremblay, 2001. Involvement of ethylene in the maturation of black spruce embryogenic cell lines with different maturation capacities. J. Exp. Bot. 52: 761–769.
Hakman, I. & L.C. Fowke, 1987. An embryogenic cell suspension culture of Picea glauca (White spruce). Plant Cell Rep. 6: 20–22.
Högberg, K.-A; Bozhkov, P.V., Grönroos, R. and S. Von Arnold.2001. Critical factors affecting ex vitro performance of somatic embryo plants of Picea abies. Scan. J. For. Res. 16: 295–304
Iraqi, D., V.Q. Le, S.M. Lamhamedi & F.M. Tremblay. Sucrose utilization during somatic embryo development in black spruce: Role of apoplastic invertase in the tissue and of extracellular invertase in the medium. J. Plant Physiol. (in press).
Iraqi, D. & F.M. Tremblay, 2001a. The role of sucrose during maturation of black spruce [Picea mariana (Mill.) BSP] and white spruce [Picea glauca (Moench) Voss] somatic embryos. Physiol. Plant.111: 381–388.
Iraqi, D. & F.M. Tremblay, 2001b. Analysis of carbohydrate metabolism enzymes and cellular levels of sugars and proteins during spruce somatic embryogenesis suggests a regulatory role of exogenous sucrose in embryo development. J. Exp. Bot. 52: 2301–2311.
Lamhamedi, M. S., Chamberland, H., Bernier, P. Y. et F. M. Tremblay, 2000. Clonal variation in morphology, growth, physiology, anatomy and ultrastructure of container-grown white spruce somatic seedlings. Tree Physiol. 20: 869–880
Lamhamedi, M.S., H. Chamberland, & F.M. Tremblay. Epidermal transpiration, ultrastructural characteristics and net photosynthesis of white spruce somatic seedlings in response to in vitro acclimatization. Physiol. Plant. (In press).
Litvay J.D., D.C. Verma & M.A. Johnson, 1985. Influence of a loblolly pine (Pinus taeda L.) culture medium and its components on growth and somatic embryogenesis of the wild carrot (Daucus carota L.). Plant Cell Rep. 4: 325–328.
Rockland L.B., 1960. Saturated salt solutions for static control of relative humidity between 5° and 40°C. Anal. Chem. 32: 1375–1376.
Tautorus, T.E. & S.M. Attree, 1990. Somatic embryogenesis from immature and mature zygotic embryos, and embryo regeneration from protoplasts in black spruce (Picea mariana Mill.). Plant Sci. 67: 115–124.
Tremblay, F.M., 1990. Somatic embryogenesis and plantlet regeneration from embryos isolated from stored seeds of Picea glauca. Can. J. Bot. 68: 236–242.
Tremblay, L., C. Levasseur & F.M. Tremblay, 1999. Frequency of somaclonal variation in plants of black spruce (Picea mariana, Pinaceae) and white spruce (P. glauca, Pinaceae) derived from somatic embryogenesis and identification of some factors involved in genetic instability. Amer. J. Bot. 86: 1373–1381.
Tremblay, L. & F.M. Tremblay, 1995. Somatic embryogenesis in black spruce (Picea mariana (Mill.) B.S.P.) and red spruce (P. rubens Sarg.). In: Y.P.S. Bajaj (Ed). Biotechnology in agriculture and forestry, vol. 30, pp. 431–445. Somatic embryogenesis and synthetic seed I, Springer-Verlag. Berlin, Heidelberg.
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Tremblay, F.M., Iraqi, D., El Meskaoui, A. (2005). Protocol of Somatic Embryogenesis: Black Spruce (Picea mariana (Mill.) B.S.P.). In: Jain, S.M., Gupta, P.K. (eds) Protocol for Somatic Embryogenesis in Woody Plants. Forestry Sciences, vol 77. Springer, Dordrecht. https://doi.org/10.1007/1-4020-2985-3_6
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