Abstract
Poly(ADP-ribosyl)ation is a post-translational modification of proteins. Upon DNA damage, poly(ADP-ribose) polymerase-1 catalyzes the transfer of ADP-ribose moieties from NAD+ onto acceptor proteins to form long and branched polymers. Poly(ADP-ribosyl)ation is an extensive but transient modification as polymer chains can reach more than 200 units on protein acceptors and be degraded within a few minutes by poly(ADP-ribose) glycohydrolase. Homeostasis of poly(ADP-ribose) is thought to play an important function in cellular processes. The importance of pADPR synthesis has been established in vitro and in vivo by using chemical inhibitors and genetically engineered mutant mice devoid of the main pADPR synthesizing enzyme, PARP-1. However, the function of PARG in vivo remains elusive. This chapter describes the generation and characterization of PARG knockout mice.
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© 2006 Landes Bioscience and Springer Science+Business Media
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Cortes, U., Wang, ZQ. (2006). Targeting the Poly (ADP-Ribose) Glycohydrolase (PARG) Gene in Mammals. In: Poly(ADP-Ribosyl)ation. Molecular Biology Intelligence Unit. Springer, Boston, MA. https://doi.org/10.1007/0-387-36005-0_3
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DOI: https://doi.org/10.1007/0-387-36005-0_3
Publisher Name: Springer, Boston, MA
Print ISBN: 978-0-387-33371-7
Online ISBN: 978-0-387-36005-8
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