Abstract
Over the past decade, the development of a series of in vitro nucleic acid ampli- fication (NAA) technologies has opened new avenues for the detection, identifi- cation, and characterization of pathogenic organisms in diagnostic microbiology (Tang et al., 1997; Jungkind and Kessler, 2002; Yolken, 2002). The promise of these techniques is the replacement of traditional biological amplification of live pathogens by enzymatic amplification of specific nucleic acid sequences. These techniques have reduced the dependency of the clinical microbiology laboratory on culture-based methods and created new opportunities for the field to enhance patient care. According to the theoretical basis for each methods, in vitro nucleic acid amplification techniques can be placed into one of three broad categories, which all share certain advantages over traditional methods, particularly for the detection of fastidious, unculturable, and/or highly contagious organisms (Table 10.1). Application of NAA techniques enhances the speed, sensitivity, and sometimes the specificity of an etiologic diagnosis (Tang et al., 1999; Yolken, 2002; Hayden, 2004).
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Li, H., Tang, YW. (2006). In Vitro Nucleic Acid Amplification: An Introduction. In: Advanced Techniques in Diagnostic Microbiology. Springer, Boston, MA. https://doi.org/10.1007/0-387-32892-0_10
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DOI: https://doi.org/10.1007/0-387-32892-0_10
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