Skip to main content
SpringerLink
Log in

Automated Blood Cultures

  • Chapter
Advanced Techniques in Diagnostic Microbiology

Abstract

A clinically suspected infection is ultimately confirmed by isolation or detection of the infectious agent. Subsequent identification of the microorganism and antibiotic susceptibility tests further guide effective antimicrobial therapy. Bloodstream infection is the most severe form of infection and is frequently life-threatening, and blood culture to detect circulating microorganisms has been the diagnostic standard. Much of the scientific and technologic advances in blood culture were made from the 1970s to the 1990s; this chapter briefly reviews various aspects of blood culture with emphasis on automated culturing systems.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 149.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 189.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Archibald, L. K., den Dulk, M. O., Pallangyo, K. J., & Reller, L. B. (1998). Fatal Mycobacterium tuberculosis bloodstream infections in febrile hospitalized adults in Dar es Salaam, Tanzania. Clin Infect Dis, 26, 290–6.

    PubMed  CAS  Google Scholar 

  • Archibald, L. K., McDonald, L. C., Rheanpumikankit, S., Tansuphaswadikul, S., Chaovanich, A., Eampokalap, B., Banerjee, S. N., Reller, L. B., & Jarvis, W. R. (1999). Fever and human immunodeficiency virus infection as sentinels for emerging mycobacterial and fungal bloodstream infections in hospitalized patients >/= 15 years old, Bangkok. J Infect Dis, 180, 87–92.

    Article  PubMed  CAS  Google Scholar 

  • Bannatyne, R. M., Jackson, M. C., & Memish, Z. (1997). Rapid diagnosis of Brucella bacteremia by using the BACTEC 9240 system. J Clin Microbiol, 35, 2673–4.

    PubMed  CAS  Google Scholar 

  • Cockerill, F. R. 3rd, Wilson, J. W., Vetter, E. A., Goodman, K. M., Torgerson, C. A., Harmsen, W. S., Schleck, C. D., Ilstrup, D. M., Washington, J. A. 2nd, & Wilson, W. R. (2004). Optimal testing parameters for blood cultures. Clin Infect Dis, 38, 1724–30.

    Article  PubMed  Google Scholar 

  • Crump, J. A., & Reller, L. B. (2003a). Two decades of disseminated tuberculosis at a university medical center: the expanding role of mycobacterial blood culture. Clin Infect Dis, 37, 1037–43.

    Article  Google Scholar 

  • Crump, J. A.,Tanner, D. C., Mirrett, S., McKnight, C. M., & Reller, L. B. (2003b). Controlled comparison ofBACTEC13A,MYCO/FLYTIC, BacT/AlertMB,and Isolator 10 systems for detection of mycobacteremia. J Clin Microbiol, 41, 1987–90.

    Article  Google Scholar 

  • De, I., Jacobson, K. L., & Han, X. Y. (2006). Species and clinical relevance of 80 strains of rapidly growing mycobacteria (manuscript in preparation).

    Google Scholar 

  • Doern, G. V., Davaro, R., George, M., & Campognone, P. (1996). Lack of requirement for prolonged incubation of Septi-Chek blood culture bottles in patients with bacteremia due to fastidious bacteria. Diagn Microbiol Infect Dis, 24, 141–3.

    Article  PubMed  CAS  Google Scholar 

  • Doern, G. V., Brueggemann, A. B., Dunne, W. M., Jenkins, S. G., Halstead, D. C., & McLaughlin, J. C. (1997). Four-day incubation period for blood culture bottles processed with the Difco ESP blood culture system. J Clin Microbiol, 35, 1290–2.

    PubMed  CAS  Google Scholar 

  • Doern, G. V., Barton, A., & Rao, S. (1998). Controlled comparative evaluation of BacT/Alert FAN and ESP 80A aerobic media as means for detecting bacteremia and fungemia. J Clin Microbiol, 36, 2686–9.

    PubMed  CAS  Google Scholar 

  • Grinsztejn, B., Fandinho, F. C., Veloso, V. G., Joao, E. C., Lourenco, M. C., Nogueira, S. A., Fonseca, L. S., & Werneck-Barroso, E. (1997). Mycobacteremia in patients with the acquired immunodeficiency syndrome. Arch Intern Med, 157, 2359–63.

    Article  PubMed  CAS  Google Scholar 

  • Han, X. Y., & Truant, A. L. (1999). The detection of positive blood cultures by the Accumed ESP-384 system: the clinical significance of three day testing. Diagn Microbiol Infect Dis, 33, 1–6.

    Article  PubMed  CAS  Google Scholar 

  • Han, X. Y., Ho, L. X., & Safdar, A. (2004a). Francisella tularensis peritonitis in stomach cancer patient. Emerg Infect Dis, 10(12), 2238–40.

    Google Scholar 

  • Han, X.Y., Meltzer, M. C., Woods, J. T., & Fainstein, V. (2004b). Endocarditis with ruptured cerebral aneurysm caused by Cardiobacterium valvarum sp. nov. J Clin Microbiol, 42, 1590–5.

    Article  Google Scholar 

  • Inderlied, C. B., Kemper, C. A., & Bermudez, L. M. (1993). The Mycobacterium avium complex. Clin Microbiol Rev, 6, 266–310.

    PubMed  CAS  Google Scholar 

  • Jorgensen, J. H., Mirrett, S., McDonald, L. C., Murray, P. R., Weinstein, M. P., Fune, J., Trippy, C. W., Masterson, M., & Reller, L. B. (1997). Controlled clinical laboratory comparison of BACTEC plus aerobic/F resin medium with BacT/Alert aerobic FAN medium for detection of bacteremia and fungemia. J Clin Microbiol, 35, 53–8.

    PubMed  CAS  Google Scholar 

  • McDonald, L. C., Fune, J., Gaido, L. B., Weinstein, M. P., Reimer, L. G., Flynn, T. M., Wilson, M. L., Mirrett, S., & Reller, L. B. (1996). Clinical importance of increased sensitivity of BacT/Alert FAN aerobic and anaerobic blood culture bottles. J Clin Microbiol, 34, 2180–4.

    PubMed  CAS  Google Scholar 

  • McDonald, L. C., Weinstein, M. P., Fune, J., Mirrett, S., Reimer, L. G., & Reller, L. B. (2001). Controlled comparison of BacT/Alert FAN aerobic medium and BACTEC fungal blood culture medium for detection of fungemia. J Clin Microbiol, 39, 622–4.

    Article  PubMed  CAS  Google Scholar 

  • Mirrett, S., Weinstein, M. P., Reimer, L. G., Wilson, M. L., & Reller, L. B. (2001). Relevance of the number of positive bottles in determining clinical significance of coagulasenegative staphylococci in blood cultures. J Clin Microbiol, 39, 3279–81.

    Article  PubMed  CAS  Google Scholar 

  • Mirrett, S., Reller, L. B., Petti, C. A., Woods, C. W., Vazirani, B., Sivadas, R., & Weinstein, M. P. (2003). Controlled clinical comparison of BacT/Alert standard aerobic medium with BACTEC standard aerobic medium for culturing blood. J Clin Microbiol, 41, 2391–4.

    Article  PubMed  Google Scholar 

  • Raad, I. I., Vartivarian, S., Khan, A., & Bodey, G. P. (1991). Catheter-related infections caused by the Mycobacterium fortuitum complex: 15 cases and review. Rev Infect Dis, 13, 1120–5.

    PubMed  CAS  Google Scholar 

  • Reimer, L. G., Wilson, M. L., & Weinstein, M. P. (1997). Update on detection of bacteremia and fungemia. Clin Microbiol Rev, 10, 444–65.

    PubMed  CAS  Google Scholar 

  • Weinstein, M. P. (1996). Current blood culture methods and systems: clinical concepts, technology, and interpretation of results. Clin Infect Dis, 23, 40–6.

    PubMed  CAS  Google Scholar 

  • Weinstein, M. P. (2003). Blood culture contamination: persisting problems and partial progress. J Clin Microbiol, 41, 2275–8.

    Article  PubMed  Google Scholar 

  • Weinstein, M. P., & Reller, L. B. (2002). Commercial blood culture systems and methods. In: Truant, A. L., ed. Manual of Commercial Methods in Clinical Microbiology. ASM Press, Washington, D C, pp. 12–21.

    Google Scholar 

  • Weinstein, M. P., Towns, M. L., Quartey, S. M., Mirrett, S., Reimer, L. G., Parmigiani, G, & Reller, L. B. (1997). The clinical significance of positive blood cultures in the 1990s: a prospective comprehensive evaluation of the microbiology, epidemiology, and outcome of bacteremia and fungemia in adults. Clin Infect Dis, 24, 584–602.

    PubMed  CAS  Google Scholar 

  • Welby-Sellenriek, P. L., Keller, D. S., Ferrett, R. J, & Storch, G. A. (1997). Comparison of the BacT/Alert FAN aerobic and the Difco ESP 80A aerobic bottles for pediatric blood cultures. J Clin Microbiol, 35, 1166–71.

    PubMed  CAS  Google Scholar 

  • Wilson, M. L. (1997). Clinically relevant, cost-effective clinical microbiology. Strategies to decrease unnecessary testing. Am J Clin Pathol, 107, 154–67.

    PubMed  CAS  Google Scholar 

  • Wilson, M. L., Mirrett, S., Meredith, F. T., Weinstein, M. P., Scotto, V., & Reller LB. (2001). Controlled clinical comparison of BACTEC plus anaerobic/F to standard anaerobic/F as the anaerobic companion bottle to plus aerobic/F medium for culturing blood from adults. J Clin Microbiol, 39, 983–9.

    Article  PubMed  CAS  Google Scholar 

Download references

Authors
  1. XIANG Y. HAN
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

Copyright information

© 2006 Springer

About this chapter

Cite this chapter

HAN, X.Y. (2006). Automated Blood Cultures. In: Advanced Techniques in Diagnostic Microbiology. Springer, Boston, MA. https://doi.org/10.1007/0-387-32892-0_1

Download citation

Publish with us

Policies and ethics

Search

Navigation