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Saturation Transfer EPR

Rotational Dynamics of Membrane Proteins

  • Chapter
Biomedical EPR, Part B: Methodology, Instrumentation, and Dynamics

Part of the book series: Biological Magnetic Resonance ((BIMR,volume 24/B))

Abstract

Saturation transfer EPR (ST-EPR) has been utilized to characterize the very slow rotational diffusion of a wide range of membrane proteins over the past three decades. Development of computational algorithms for analyzing the ST-EPR data from membrane proteins undergoinguniaxial rotational diffusion and inclusion of these algorithms into global nonlinear least squares fitting routines has permitted detailed analyses of experimental data. These advances, coupled with access to multifrequency measurements, has established ST-EPR as an important complement to transient optical anisotropy for unraveling the complex rotational dynamics of intrinsic membrane proteins in cell membranes.

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Authors and Affiliations

  1. Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA

    Albert H. Beth & Eric J. Hustedt

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  1. Albert H. Beth
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  1. University of Denver, Denver, Colorado

    Sandra R. Eaton , Gareth R. Eaton  & Lawrence J. Berliner ,  & 

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© 2005 Kluwer Academic/Plenum Publishers

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Beth, A.H., Hustedt, E.J. (2005). Saturation Transfer EPR. In: Eaton, S.R., Eaton, G.R., Berliner, L.J. (eds) Biomedical EPR, Part B: Methodology, Instrumentation, and Dynamics. Biological Magnetic Resonance, vol 24/B. Springer, Boston, MA. https://doi.org/10.1007/0-306-48533-8_12

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  • DOI: https://doi.org/10.1007/0-306-48533-8_12

  • Publisher Name: Springer, Boston, MA

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