Abstract
Comparing several techniques for creating epidermal replicas, we found that imprints on cellulose-di-acetate and on polymethyl-metacrylate can be easily performed within a few seconds, which proves to be a considerable advantage especially in field trials. The result is a permanent impression of the epidermis’ surface, perfect for long-term storage. In the case of extremely sunken stomata, pleated leaf surfaces, or coniferous needles, we additionally used a cyanacrylate adhesive. Reliable and reproducible results could be achieved for use when analysing the imprints by a drawing microscope or an image analyses program.
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References
Barlow P.W., Carr D.J., eds. Positional Controls in Plant Development. Cambridge: Cambridge University Press, 1984
Bettarini I., Vaccari F.P., Miglietta F. Elevated CO 2 concentrations and stomatal density: observations from 17 plant species growing in a CO 2 spring in central Italy. Global Change Biol 1998; 4:17–22
Bolhàr-Nordenkampf H.R., Draxler G. “Functional Leaf Anatomy”. In Photosynthesis and Production in a Changing Environment. D.O. Hall, J.M.O. Scurlock, H.R. Bolhàr-Nordenkampf, R.C. Leegood, S.P. Long, eds. London: Chapman and Hall, 1993
Bunce J.A. Stomatal conductance, photosynthesis and respiration of temperature deciduous tree seedlings grown outdoors at an elevated concentration of carbon dioxide. Plant Cell Environ 1992; 15:541–549
Bunce J.A. Responses of stomatal conductance to light, humidity and temperature in winter wheat and barley grown at three concentrations of carbon dioxide in the field. Global Change Biol 1999; 6:371–382
Bünning E. Die Entstehung von Mustern in der Entwicklung von Pflanzen. Encyclopedia of Plant Physiol 1965; 15:383–403
Cousson A. Analysis of the sensing and transducting processes implicated in the stomatal responses to carbon dioxide in Commelina communis L. Plant Cell Environ 2000; 23:487–495
Esau K. Plant Anatomy. Ed 2, John Wiley and Sons, Inc., 1965
Fahn A. Plant Anatomy. Ed 3, Pergamon Press, 1982
Gay A.P., Hauck B. Acclimation of Lolium temulentum to enhanced carbon dioxide concentration. J Exp Bot 1994; 45:1133–1141
Jarvis P.G., Mansfield T.A., Davies W.J. Stomatal behaviour, photosynthesis and transpiration under rising CO 2 . Plant Cell Environ 1999; 22:639–648
Körner C Does global increase of CO 2 alter stomatal density? Flora 1988; 181:253–257
Körner C., Neumayer M., Menendez-Riedl S., Smeets-Scheel A. Functional morphology of mountain plants. Flora 1989; 182:353–383
Leuning R. A critical appraisal of a combined stomatal-photosynthesis model for C 3 plants: theoretical paper. Plant Cell Environ 1995; 18:339–355
Mauseth J.D. Plant Anatomy. The Benjamin/Cummings Publishing Company, Inc., 1988
Martin E.S., Donkin M.E., Stevens R.A. Stomata. London, UK: Edward Arnold Publishers Limited, 1983
Metcalfe C.R., Chalk L. Anatomy of the Dicotyledons. Vol 1. Oxford: Oxford University Press, 1950
Morison J.I.L. Plant growth and CO 2 history. Nature 1987; 327:560
Oren R., Sperry J.S., KatuI G.G., Pataki D.E., Ewers B.E., Phillips N., Schäfer K.V.R. Survey and synthesis of intra-and interspecific variation in stomatal sensitivity to vapour pressure deficit. Plant Cell Environ 1999; 22:1515–1526
Radoglou K.M., Jarvis P.G. Effects of CO 2 enrichment on four poplar clones. I. Growth and leaf anatomy. Ann Bot 1990; 65:617–626
Radoglou K.M., Jarvis P.G. Effects of CO 2 enrichment and nutrient supply on growth, morphology and anatomy of Phaseolus vulgaris L. seedlings. Ann Bot 1992; 70:245–256
Raschke K. Stomatal action. Annu Rev Plant Physiol 1975; 26:309–340
Salisbury E.J. Leaf form and function. Nature 1949; 163:515–518
Thomas G.F., Harvey C.N. Leaf anatomy of four species grown under continuous CO 2 enrichment. Bot Gazette 1983; 144:303–309
Ticha I., Obermajer P., Snopek J. Stomata density and sizes in in vitro grown tobacco plantlets. Physiol Plantarum 1997; 29:101–107
Tognetti R., Longobucco A., Miglietta F., Raschi A. Transpiration and stomatal behaviour of Quercus ilex plants during the summer in a Mediterranean carbon dioxide spring. Plant Cell Environ 1998; 21:613–622
Vazzana C., Puliga S., Bochicchio A., Raschi A. Study of the stomatal density in an alfalfa (Medicago sativa L.) ecotype. Rivista di Agronomia 1988; 22:127–132
Willmer C.M. Stomata. Longman Group Limited, 1983
Woodward F.I. Stomatal numbers are sensitive to increasing in CO 2 from preindustrial levels. Nature 1987; 327:617–618
Woodward F.I., Bazzaz F.A. The responses of stomatal density to CO 2 partial pressure. J Exp Bot 1988; 39:1771–1781
Woodward F.I., Kelly C.K. The influence of CO 2 concentration on stomatal density. New Phytol 1995; 131:311–327
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Meister, M.H., Bolhàr Nordenkampf, H.R. (2001). Stomata Imprints: A New and Quick Method to Count Stomata and Epidermis Cells. In: Reigosa Roger, M.J. (eds) Handbook of Plant Ecophysiology Techniques. Springer, Dordrecht. https://doi.org/10.1007/0-306-48057-3_17
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DOI: https://doi.org/10.1007/0-306-48057-3_17
Publisher Name: Springer, Dordrecht
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