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“2+1” Pulse Sequence as Apllied for Distance and Spatial Distribution Measurements of Paramagnetic Centers

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Distance Measurements in Biological Systems by EPR

Part of the book series: Biological Magnetic Resonance ((BIMR,volume 19))

Abstract

Applications of one of the varieties of electron spin-echo method, the “2+1” pulse sequence used for the measurement of distances in radical pairs with fixed and distributed distances, and for the determination of the parameters of spatial distribution in more complicated cases (fractals, clusters, non-random distributions) are discussed. Special attention is given to features of the “2+1” technique such as suppression of dipolar interactions of randomly distributed spins, which allows the selection of a dipolar interaction between radical labels in proteins, even if protein molecules aggregate. Experimental conditions that allow the separation of nuclear modulation from electron dipolar related modulation, or minimize the appearance of ESEEM in a “2+1” experiment are also discussed.

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Authors and Affiliations

  1. Department of Chemistry, University of Arizona, Tucson, AZ, 85721, USA

    A. Raitsimring

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  1. A. Raitsimring
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Editors and Affiliations

  1. Ohio State University, Columbus, Ohio, USA

    Lawrence J. Berliner

  2. Department of Chemistry and Biochemistry, University of Denver, 80208, Denver, Colorado, USA

    Gareth R. Eaton  & Sandra S. Eaton  & 

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© 2002 Kluwer Academic / Plenum Publishers, New York

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Raitsimring, A. (2002). “2+1” Pulse Sequence as Apllied for Distance and Spatial Distribution Measurements of Paramagnetic Centers. In: Berliner, L.J., Eaton, G.R., Eaton, S.S. (eds) Distance Measurements in Biological Systems by EPR. Biological Magnetic Resonance, vol 19. Springer, Boston, MA. https://doi.org/10.1007/0-306-47109-4_10

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  • DOI: https://doi.org/10.1007/0-306-47109-4_10

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4757-0575-1

  • Online ISBN: 978-0-306-47109-4

  • eBook Packages: Springer Book Archive

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