Abstract
CHO cells were successfully cultured on a novel type of microcarriers (ImmobaSil FS) in the bioreactors. Various factors influencing cell attachment, including serum concentration, cell to microcarrier ratio, agitation speed and agitation profile were investigated and the results were compared with those obtained using CultiSpher-G, and glass beads. It was found that the optimum serum concentration, cell-to- carrier ratio and agitation speed for the batch reactor with a working volume of 400 ml during the initial attachment period were 10% (vh), 12×103∶1 and 60 rpm, respectively. Intermittent stirring/non-stirring regimen of 20/40 minutes resulted in an optimum rate of cell attachment (0.27 h−1). However, intermittent and continuous stirring schemes had no significant effect on the total number of attached cells. ImmobaSil FS carriers performed better than CultiSpher-G and glass beads in term of the rate of cell attachment.
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© 2002 Kluwer Academic Publishers
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Hu, A.Y., Zhang, Z., Al-Rubeal, M. (2002). Cultivation of CHO Cells on Novel Microcarriers. In: Ikura, K., Nagao, M., Masuda, S., Sasaki, R. (eds) Animal Cell Technology: Challenges for the 21st Century. Springer, Dordrecht. https://doi.org/10.1007/0-306-46869-7_8
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DOI: https://doi.org/10.1007/0-306-46869-7_8
Publisher Name: Springer, Dordrecht
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