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Engineering Skin to Study Human Disease – Tissue Models for Cancer Biology and Wound Repair

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Tissue Engineering II

Part of the book series: Advances in Biochemical Engineering/Biotechnology ((ABE,volume 103))

Abstract

Recent advances in the engineering of three-dimensional tissues known as skin equivalents, that have morphologic and phenotypic properties of human skin, have provided new ways to study human disease processes. This chapter will supply an overview of two such applications – investigations of the incipient development of squamous cell cancer, and studies that have characterized the response of human epithelium during wound repair. Using these novel tools to study cancer biology, it has been shown that cell-cell interactions inherent in three-dimensional tissue architecture can suppress early cancer progression by inducing a state of intraepithelial dormancy. This dormant state can be overcome and cancer progression enabled by altering tissue organization in response to tumor promoters or UV irradiation or by modifying the interaction of tumor cells with extracellular matrix proteins or their adjacent epithelia. By adapting skin equivalent models of human skin to study wound reepithelialization, it has been shown that several key responses, including cell proliferation, migration, differentiation, growth-factor responsiveness and protease expression, will mimic the response seen in human skin. In this light, these engineered models of human skin provide powerful new tools for studying disease processes in these tissues as they occur in humans.

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Abbreviations

IE:

Intraepithelial

ECM:

Extracellular matrix

β-gal:

β-Galactosidase

BM:

Basement membrane

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Authors and Affiliations

  1. Division of Cancer Biology and Tissue Engineering Department of Oral and Maxillofacial Pathology, Tufts University, 55 Kneeland Street, Room 116, 02111, Boston, Massachusetts, USA

    Jonathan A. Garlick

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  1. Jonathan A. Garlick
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Correspondence to Jonathan A. Garlick .

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Kyongbum Lee David Kaplan

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© 2006 Springer-Verlag Berlin Heidelberg

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Garlick, J.A. (2006). Engineering Skin to Study Human Disease – Tissue Models for Cancer Biology and Wound Repair. In: Lee, K., Kaplan, D. (eds) Tissue Engineering II. Advances in Biochemical Engineering/Biotechnology, vol 103. Springer, Berlin, Heidelberg . https://doi.org/10.1007/b137206

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