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Micropropagation of tea (Camellia sinensis L.)

  • Chapter
Micropropagation of Woody Trees and Fruits

Part of the book series: Forestry Sciences ((FOSC,volume 75))

Abstract

Tea (Camellia sinensis L.), of family Theacea is the oldest non-alcoholic caffeine containing beverage crop in the world. Chinese were the first to use tea as a medicinal drink, later as beverage and have been doing so for the past 3000 years (Eden, 1958). Today, tea has become the most popular and cheapest beverage drink world-wide. The cultivated taxa comprise of three main natural hybrids which are: C. sinensis (L.) O. Kuntze or China tea, C. assamica (Masters) or Assam tea and C. assamica sub sp. lasiocalyx (Planchon ex Watt.) or Cambod tea or Southern tea. Tea is an evergreen, perennial, cross-pollinated plant and grows naturally as tall as 15 meters. However, under cultivated condition, the bush height 60-100 cm is maintained for harvesting the young leaves, which continues even more than 100 years. The flowers are white in colour and born singly or in pairs at the axils. The fruits are green in colour with 2-3 seeds and start bearing within 5-6 years after planting. Leaf is the main criterion by which three types of tea are classified. Briefly, a) Assam type has biggest leaves, b) China type has smallest leaves, and c) Cambod leaves size are in-between of both, Assam and China type.

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References

  • Abraham G C, Raman K. (1986) Somatic embryogenesis in tissue culture of immature cotyledons of tea (Camellia sinensis). In: Somers D.A., Gengenbach B.G., Biesboor D.D., Hackett W.P., Green C.E. (eds) 6th Int. Congr.on Plant Tissue and Cell Culture. Univ. Minnesota, Minneapolis. pp. 294.

    Google Scholar 

  • Agarwal B., Singh U., Baneijee M. (1992) In vitro clonal propagation of tea (Camellia sinensis (L.) O. Kuntze). Plant Cell Tissue Org. Cult. 30: 1–5.

    Article  CAS  Google Scholar 

  • Akula A., Dodd W. A. (1998) Direct somatic embryogenesis in a selected tea clone, ‘TRI-2025’(Camellia sinensis (L).O. Kuntze) from nodal segment. Plant Cell Rep. 17: 804–809.

    Article  CAS  Google Scholar 

  • Akula A., Akula C. (1999) Somatic embryogenesis in tea (Camellia sinensis (L) O Kuntze. In: Jain S.M., Gupta P.K., Newton R. J. (eds) Somatic embryogenesis in woody plants, Vol. 5. KluwerAcademic Publishers, The Greate I3ritain, pp. 239–259.

    Chapter  Google Scholar 

  • Akula A., Akula C., Bateson M. (2000) Betaine a novel candidate for rapid induction of somatic embryogenesis in tea (Camelia sinensis (L.) O.Kuntze). Plant Growth Regu. 30: 241–246.

    Article  CAS  Google Scholar 

  • Annual Report (1999). Research and Development Department, Tata Tea Ltd, Munnar, India

    Google Scholar 

  • Arulpragasam P.V., Latiff R., (1986) Studies on the tissue culture on tea (Camelia sinensis (L.) O. Kuntze). 1. Development of a culture method for the multiplication of shoots. Sri Lank J. Tea Sci. 55: 44–47.

    Google Scholar 

  • Arulpragasam P. V., Latiff R., Seneviratne P. (1988) Studies on the tissue culture of tea (Camellia sinensis (L.) O. Kuntze). 3. Regeneration of plants from cotyledon callus. Sri Lank J. Tea Sci. 57: 20–23.

    Google Scholar 

  • Avgioglu A., Knox R. B. (1989) Storage lipid accumulation by zygotic and somatic embryo in culture. Ann. Bot. 63: 409–412.

    Google Scholar 

  • Bag N., Palni L.M.S., Nandi S. K. (1997) Mass propagation of tea using tissue culture methods. Physiol. Mol. Biol. Plants. 3: 99–103.

    Google Scholar 

  • Balasubramanian S., Marimuthu S., Rajkumar R., Haridas V. (2000) Somatic embryogenesis and multiple shoot induction in Camellia sinensis (L.) O. Kuntze. J. Plant Crops. 28: 44–49.

    Google Scholar 

  • Banerjee A. K., Agrawal D. C., Nalawade S. M., Krishnamurty K. V. (2000) Recovery of in vitrocotton shoots through micrografting. Cuir. Sci. 78: 623–626.

    Google Scholar 

  • Banerjee B. (1992) Botanical classification of tea. In: Wilson K.C., Clifford M.N. (eds) Tea cultivation to consumption. Chapman and Hall, London, pp. 25–51.

    Google Scholar 

  • Banerjee M., Agarwal B. (1990) In vitro rooting of tea, Camellia sinensis (L.) O. Kuntze. Ind. J. Exp. Biol. 28: 936–939.

    CAS  Google Scholar 

  • Bano Z., Rajaratnam S., Mohanty B. D. (1991) Somatic embryogenesis in cotyledon culture of tea (Thea sinensis L.) J. Hort, Sci, 66: 465–470.

    Google Scholar 

  • Beretta D., Vanoli M., Eccher T. (1987) The influence of glucose, vitamins and IBA on rooting of Camellia shoots in vitro. Abstr. Symp. on Vegetative Propagation of Woody Species, Pisa, Italy p 105. Bezbaruah H. P. (1971) Cytological investigation in the family theaceae-I. Chromosome numbers in some Camellia species and allied genera. Carylogia. 24: 421–426.

    Google Scholar 

  • Bhatia C.R., Murty G.S.S., Mouli C., Kale D. M. (1986) Nuclear techniques and in vitro culture for plant improvement. In: Proceedings of an International sympossium on nuclear techniques and in vitro culture for plant improvement. IAEE, FAO and UN, Vienna, 19–23 August. pp. 19–23.

    Google Scholar 

  • Chaudhaury R., Lakhanpal S., Chandel K. P. S. (1990) Germination and desiccation tolerance of tea (Camellia sinensis (L.) O. Kuntze) seeds and feasibility of cryopreservation. Sri Lank J Tea Sci. 59: 89–94.

    Google Scholar 

  • Chaudhury R., Radhamani J., Chandel K.P.S. (1991) Preliminary observation in the cryo-preservation of desiccated embryonic axes of tea (Camellia sinensis) L.O.Kuntze) seeds for genetic conservation. Cryo-letters 12:31–36.

    Google Scholar 

  • Chen Z. (1999) Pharmalogical functions of tea. In: Jain N.K. (ed) Global advances in tea Science, Aravali Books International (P) Ltd, New Delhi, pp.333–358.

    Google Scholar 

  • Chen Z., Liao H. (1982) Obtaining plantlet through anther culture of tea plants. Zhongguo chaye. 4:67.

    Google Scholar 

  • Chen Z., Liao H. (1983) A success in bringing out tea plants from the anthers. China Tea 5:6–7. Corley R.H.V., Lee C.H., Wong, C.V. (1986) Abnormal flower development in oil palm clones. Planter 62: 6–7.

    Google Scholar 

  • Creze J., Beauchesne M. G. (1980) Camellia cultivation in vitro. Int. Camellia J. 12: 31–34.

    Google Scholar 

  • Damasco O.P., Godwin I.D., Smith M.K., Adkins S.W. (1996) Gibberellic acid detection of dwarf off_ types in micropropagated Cavendish bananas. Aus. J. Expt. Agric. 36: 237–241.

    Article  Google Scholar 

  • Das S. C.(1992) Non-conventional techniques of regenerating polyploids in tea. Proc. 31St Tocklai Con£, TRA, Jorhat; 1992: 26–30.

    Google Scholar 

  • Das S. C. (2001) Tea. In: Parthasarathy VA., Bose TK., Deka PC., Das P., Mitra SK., Mohandas S. Biotechnology of Horticultural Crops, Vol. 1. (eds) Naya Prokash., India, pp. 526–546.

    Google Scholar 

  • Das S.C., Barman T. S. (1988) Current state and future potential of tissue culture in tea. Proc. 30th Tocklai Conf. TRA Jorhat, pp. 90–94.

    Google Scholar 

  • Das S.C., Barman T.S., Singh R. (1990) Proc. Int. Conf. Tea Res. Assoc. Global Perspective, Januaryl 1–12, 1990, TRA, Calcutta, pp. 1–12.

    Google Scholar 

  • Debergh P.C., Vanderschaeghe A. M. (1988) Some symptoms indicating the presence of bacterial contaminants in plant tissue culture. Acta Hort. 255: 77–81.

    Google Scholar 

  • Dood A. W. (1994). Tissue culure of tea (Camellia sinensis (L.) O.Kuntze) — a review. Int. J. Trop Argic. XII (3 & 4) 212–247.

    Google Scholar 

  • Eden T. (1958) The development of tea culture. In: Eden T. (ed) Tea, Longman, London pp. 1–4.

    Google Scholar 

  • Evans D.A., Sharp W.R. (1986) Somaclonal and gametoclonal variation: In: Evans DA., Sharp W.R., Ammirato P.V. (eds) Hand Book of plant cell culture, Vol.4. Technique and applications, Macmillan Publishing Company, New York. pp. 97–132.

    Google Scholar 

  • Florin B., Tessereau H., Leconteux C., Didier C., Petiard V. (1993) Long term preservation of somatic embryos. In: Redenbaugh K (ed) Synseeds: application of synthetic seeds to crop improvement (pp133–162) CRC press, USA.

    Google Scholar 

  • Forrest G.I. (1969) Studies on the polyphenol metabolism of tissue culture derived from the tea plant(C. sinensis L.) Biochem.J. 113: 765–772

    CAS  Google Scholar 

  • Gamborg O., Miller R., Ojima K. (1968) Nutrient requirements of suspension cultures of soyabean root cells. Exp. Cell. Res. 50: 157–158.

    Article  Google Scholar 

  • Ghost’ B., Sen S. (1994) Plant regeneration from alginate encapsulated somatic embryos of Asperagus cooperi baker. Plant Cell Rep. 13, 381–385.

    Google Scholar 

  • Groose R.W., Bingham E.T. (1984) Variation in plants regenerated from tissue culture of tetraploid alfalfa heterozygous for several traits. Crop Sci. 24: 655–658.

    Article  Google Scholar 

  • Haldeman JH., Thomas R.L., Mckainy D.L. (1987) Use of benomyl and rifampicin for in vitro shoot tip culture of Camellia sinensis and Camellia japonica. Hort. Sci. 22: 306–307.

    CAS  Google Scholar 

  • Hammerschlag F. A. (1992) Somaclonal variation: In: Hammerschlag, F. A., Lit R.E. (eds) Biotechnology of perennial fruit crops. C.A.B International, pp. 35–55.

    Google Scholar 

  • Haridas V., Balasaravanan T., Rajkumar R., Marimuthu S. (2000) Factor influencing somatic embryogenesis in Camellia sinensis (L.) O.Kuntze. In: Muraleedharan A., Rajkumar R. (eds) Recent Advances in Plantation Crops Research. Allied Publishers Ltd, India, pp. 31–35.

    Google Scholar 

  • Harry J.S., Gene J.G., Zimmerman RH. (1983) Field performance and phenotypic stability of tissue culture propagated thornless blackberries. J. Amer. Soc. Hort. Sci. 108: 285–290; 1983.

    Google Scholar 

  • Heller R. (1953) Recherches sur la nutrition minerale des tissus vegetaux cultives in vitro Annales des Sciences Naturelles (Botanique) Biologie Vegetate. 14: 1–223.

    Google Scholar 

  • Hu H. (1983) Genetic stability and variability of pollen derived plants. In: Sen S.K., Giles K.L. (eds) Plant cell culture in crop improvement. Plenum Press, New York, pp. 145–157.

    Google Scholar 

  • Iddagoda N., Kataeva N.N., Butenko R. G. (1988) In vitro clonal micropropagation of tea (Camellia sinensis L.) 1. Defining the optimum condition for culturing by means of a mathematical design techniques. Ind. J. Plant Physiol. 31: 1–10.

    Google Scholar 

  • Ikekawa N. (1991) In: Patterson G.W., Nes W.D. (eds) Physiology and biochemistry of sterols American Oil chemists Society. Champaign. Vol. II. pp. 347–360.

    Google Scholar 

  • Isabel N.L., Tremblay M.M., Tremblay F.M., Bousquet. J. (1993) RAPD as an aid to evalute the genetic integrity of somatic embryogenesis derived population of Picea mariana (Mill) B.S.P. Theor. Appl. Genet. 86:81–87

    Article  CAS  Google Scholar 

  • Jain S.M., Newton R. J. (1990) Prospects of biotechnology for tea improvement. Proc. Indian. Natl. Sci. Acad. 6:441–448.

    Google Scholar 

  • Jain SM., Das S.C., Barman T.S. (1991) Induction of roots from regenerated shoots of tea (Camellia sinensis L.). Acta Hort. 289:339–340.

    Google Scholar 

  • Jain S.M., Das S.C., Barman T.S. (1993) Enhancement of root induction from in vitro regenerated shoots of tea (Camellia sinensis L.) Proc. hid. Nail. Sci. Acad. 59:623–628.

    Google Scholar 

  • Jha T.B. Sen S.K. (1992) Micropropagation of an elite Darjeeling tea clone. Plant Cell Rep. 11:101–104.

    Article  Google Scholar 

  • Jha T.B., Jha S., Sen S.K. (1992) Somatic embryogenesis from immature cotyledon of an elite Darjeeling tea clone. Plant Sci. 84:209–213.

    Article  CAS  Google Scholar 

  • Kato M. (1982) Results of organ culture on Camellia japonica and C. sinensis. Jpn. J. Breed. 32: 267–277.

    Google Scholar 

  • Janeiro, L.V., Ballester, A. and Vieitez, A.M., 1995. Effect of cold storage on somatic embryogenesis systems of Camellia. J. Hort. Science, 70 (4): 665–672.

    Google Scholar 

  • Kato M. (1985) Regeneration of plantlets from tea stem callus. Jpn. J. Breed. 35: 317–322.

    Google Scholar 

  • Kato M. (1986a) Micropropagation through cotyledon culture in Camellia japonica L. and Camellia sinensis L. Jpn. J. Breed. 36:31–38.

    CAS  Google Scholar 

  • Kato M. (1989a) Camellia sinensis L. (Tea):In vitroregeneration. In: Bajaj Y.S.P (ed) Biotechnology in Agriculture and Forestry, Vol. 7. Medicinal and Aromatic Plants Vol. II. Springer-Verlag, Berlin, Heidelberg, pp. 83–98.

    Google Scholar 

  • Kato M. (1989b) Polyploids of Camellia through culture of somatic embryos. Hort. Sci., 24: 1023–1025.

    Google Scholar 

  • Kato M. (1996) Somatic embryogenesis from immature leaves of in vitro grown tea shoots. Plant Cell Rep. 15:920–923.

    Article  CAS  Google Scholar 

  • Kuranuki Y., Sakai A. (1995) Cryo-preservation of in vitro grown shoot tips of tea (Camellia sinensis) by vitrification. Cryo-letters. 16:345–352.

    Google Scholar 

  • Kuranuki Y., Shibata M. (1992) Effect of concentration of plant growth regulators on the shoot apex culture of tea plant. Bull. Shizuoka Tea Expt. Station. 16:1–6.

    Google Scholar 

  • Kuranuki Y., Shibata M. (1993) Improvement of medium components for in vitro cuttings of tea plant. 2. Optimum concentration of plant growth regulators. J. Tea Sci. 77: 39–45.

    Google Scholar 

  • Kuranuki Y., Yoshida S. (1991) Cryopreservation of tea seeds and excised embryonic axes in liquid nitrogen. Proc. Int. Symp. Tea Sci. Shizuoka, Japan. pp 419–420.

    Google Scholar 

  • Lecouteux C.G., Lai F.M., Mckersie B.D. (1993) Maturation of alfalfa (Medicago sativa L.) somatic embryos by abscisic acid, sucrose and chilling stress. Plant Science. 94:207–213.

    Article  CAS  Google Scholar 

  • Llyod G., McCown B. (1980) Commercially feasible micropropagation of mountain laurel, Kalmia lalifolia by use of shoot tip culture. Comb. Proc. Mt. Pl. Prop. Soc. 30:421–427.

    Google Scholar 

  • Marcatrigiano M., Boyle T.H., Morgan P.A., Ambach, K.L. (1990) Leaf color variants from coleus shoots cultures. J. Amer. Soc. Hort. Sci. 115: 681–696.

    Google Scholar 

  • Maugh T. H. (1981) The natural occurring brassionoide in the plant species. Science. 212: 33–34.

    Article  PubMed  Google Scholar 

  • Mondal TK., Bhattacharya A., Sood A., Ahuja P.S. (1998) Micropropagation of tea using thidiazuran. Plant Growth Regu. 26: 57–61.

    Article  Google Scholar 

  • Mondai T.K., Bhattachraya A., Sood A., Ahuja P.S. (1999) An efficient protocol for somatic embryogenesis and its use in developing transgenic tea (Camellia sinensis (L) O. Kuntze) for field transfer. In: Altman A., Ziv M., Izhar S. (eds) Plant Biotechnology and In Vitro Biology in 21st Century. Kluwer Academic Publishers, Dordrecht, The Netherlands, pp. 101–104.

    Google Scholar 

  • Mondal TK., Bhattacharya A., Sood A., Ahuja P.S. (2000b) Factor effecting induction and storage ofencapsulated tea (Camellia sinensis L. O.Kuntze) somatic embryos. Tea. 21: 92–100.

    Google Scholar 

  • Mondai T.K., Bhattacharya A., Ahuja P.S. (2001a) Induction of synchronous secondaryembryogenesis of Tea (Camellia sinensis). J. Plant Physiol. 158: 945–951.

    Article  Google Scholar 

  • Mondal TK., Bhattachray A., Sharma M., Ahuja P.S. (2001b) Induction of in vivo somatic embryogenesis in tea (Camellia sinensis) cotyledons. Curr. Sci, 81: 101–104.

    Google Scholar 

  • Mondai T.K., Chand P.K. (2001 f) Detection of genetic instability among the miocropropagated tea (Camellia sinensis) Plants. In Vitro Cell and Dev Biol. 37:1–5.

    Article  Google Scholar 

  • Mondai TK., Bhattacharya A., Ahuja P.S., Chand P.K. (2001h) Factor effecting Agrobacterium tumefaciens mediated transformation of tea (Camellia sinensis (L).O.Kuntze) Plant Cell Rep. 20:712–720.

    Google Scholar 

  • Mondai TK., Bhattachrya A., Sood A., Ahuja P.S. (2002a) Higher conservation frequency of somatic embryos for improved transformation efficiency in tea (Camellia sinensis (L.) O. Kuntze) J. Plant Physiol. (In press).

    Google Scholar 

  • Mondai T.K., Bhattacharya A., Sood A., Ahuja P.S. (2002b) Cold treatment and storage of synthetic seed of tea (Camellia sinensis). Curr. Sci. (Accepted).

    Google Scholar 

  • Mondai T.K., Parathi R. (2002c) Micrografting - A technique to shorten the hardening time of in vitro raised tea plants. J Hort and Biotech. (Communicated)

    Google Scholar 

  • Mondai. T.K., Bhattacharya A., Laxmikumaran L. (2002d). Recent advance of Camellia Biotechnology. Plant Cell Tissu Org Cult. (Communicated).

    Google Scholar 

  • Moore P. P., Robbins J.A., Sjulin T.M. (1991) Field performance of “Olympus” strawberry subclones. Hort. Sci. 26:192–194.

    Google Scholar 

  • Murali K.S., Pandidurai V., Manivel L., Rajkumar R. (1996) Clonal variation in multiplication of tea through tissue culture. J. Plant Crops. 24: 517–522.

    Google Scholar 

  • Muralidharan E.M., Mascarenhas A.F. (1995) Somatic embryogenisis in Eucalyptus. In: Jain S., Gupta P.K., Newton R. (eds) Somatic embryogenesis in woody plant. Vol. 2. Angiosperm. Kluwer Academic Publishers. Dordrecht. The Netherlands, pp. 23–40.

    Chapter  Google Scholar 

  • Murashige T., Skoog F. (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant. 15: 473–497.

    Article  CAS  Google Scholar 

  • Nadamitsu S., Andoh Y., Kondo K., Segawa M. (1986) Interspecific hybrids between Camellia vietnamensis and C. chrysantha by cotyledon culture. Jpn. J. Breed. 36: 309–313.

    Google Scholar 

  • Nakamura Y. (1990) Effect of sugar on formation of adventitious buds and growth of axillary buds in tissue culture of tea. Bull. Shizuoka Tea Expt. Station. 15: 1–5.

    Google Scholar 

  • Nakamura Y. (1985) Effect of origin of explants on differentiation of root and its varietal difference in tissue culture of tea plant. Tea Res. J. 62: 1–8.

    Article  Google Scholar 

  • Nakamura Y. (1987a) Shoot tip culture of tea cultivar Yabukita. Tea Res. J. 65: 1–7.

    Article  Google Scholar 

  • Nakamura Y. (1987b) In vitro rapid plantlet culture from axillary buds of tea plant (C. sinensis (L.) O. Kuntze). Bull. Shizuoka Tea Expt. Station. 13: 23–27.

    Google Scholar 

  • Nakamura Y. (1988a) Efficient differentiation of adventitious embryos from cotyledon culture of Camellia sinensis and other Camellia species. Tea Res. J. 67: 1–12.

    Google Scholar 

  • Nakamura Y. (1988b) Effects of the kinds of auxins on callus induction and root differentiation from stem segment culture of Camellia sinensis (L.) O. Kuntze. Tea. Res. J. 68: 1–7.

    Google Scholar 

  • Nakamura Y. (1989a) Differentiation of adventitious buds and its varietal difference in stem segment culture of Camellia sinensis (L.) O. Kuntze. Tea Res. J. 70: 41–49.

    Article  Google Scholar 

  • Nitsch J.P., Nitsch C. (1969) Haploid plants from pollen grains. Science. 163: 185.

    Article  Google Scholar 

  • Ogutuga D.B.A., Northcote D.H. (1970a) Caffeine formation in tea callus tissue. J. Expt Bot. 21: 258–273.

    Google Scholar 

  • Palni L.M.S., Bag N., Nadeem M., Tamata S., Vyas P., Bisht MS., Purohit V.K., Kumar A., Nandi S.K., Pandey A., Purohit A.N. (1998). Micropropagation: Conservation through tissue culture of selected Himalayan Plants. In: Research for Mountain Development: Some initiatives and accomplishments. Nainital: Gyanodaya Prakashan, pp431–452.

    Google Scholar 

  • Pandey A., Bag N., Chandra, B., Palni L.M.S. (2002) Biological hardening: a promising technology for tissue culture industry. In: Role of plant tissue culture in biodiversity conservation and economic development. Nainital: Gyanodaya Prakashan, pp565–577.

    Google Scholar 

  • Pandey A., Palni L.M.S., Bag N. (2000). Biological hardening of tissue culture raised tea plants through rhizosphere bacteria. Biotech. Letter. 22: 1087–1091.

    CAS  Google Scholar 

  • Pandidurai V., Murali K.S., Manivel L., Rajkumar R. (1996) Factors affecting in vitro shootmultiplication and root regeneration in tea. J. Plantation Crops 24 (Suppl): 603–609.

    Google Scholar 

  • Paratasilpin T. (1990) Comparative studies on somatic embryogenesis in Camellia sinensis var.sinensis and C. sinensis var. assamica (Mast) Pierre. J. Sci. Soc. Thailand. 16: 23–41.

    Article  Google Scholar 

  • Phukan M.K., Mitra G.C. (1984) Regeneration of tea shoots from nodal explants in tissue culture.Curr. Sci. 53: 874–876.

    Google Scholar 

  • Phukan M.K., Mitra G.C. (1990) Nutrient requirements for growth and multiplication of tea plants in vitro. Bangladesh J. Bot. 19: 65–71.

    Google Scholar 

  • Ponsamuel J., Samson NP., Ganeshan P.S., Satyaprakash V., Abrahan G.C. (1996) Somatic embryogenesis and plant regeneration from the immature cotyledonary tissues of cultivated tea (Camellia sinensis (L.) O. Kuntze). Plant Cell Rep. 16: 210–214.

    Article  CAS  Google Scholar 

  • Prakash O., Sood A., Sharma M., Ahuja P.S. (1999) Grafting micropropagated tea (Camellia sinensis (L.) O. Kuntze) shoots on tea seedling-a new approach to tea propagation. Plant Cell Rep. 18: 137–142.

    Google Scholar 

  • Rajkumar R., Ayyappan P. (1992) Somatic embryogenesis from cotyledonary explants of Camellia sinensis (L.) O. Kuntze. The Planters Chronic. May. 227–229.

    Google Scholar 

  • Rajkumar R., Ayyappan P. (1992) Micrppropagation of Camellia sinensis (L.) O kuntze. J Plant Crops 20: 252–256.

    Google Scholar 

  • Rajkumar R., Balasusaravanam S., Jayakumar D., Haridas V., Marimuthu S. (2001). Physiological and biochemical feathers of field grown somaclonal variants of Tea. UPASI tea research foundation Bulletin No 54, 73–81.

    Google Scholar 

  • Rajkumar R., Ayyappan P. (1992) Micropropagation of Camellia sinensis (L.) O.Kuntze. J Plant. Crops 20: 252–256.

    Google Scholar 

  • Rajashekaran P., Mohankumar P. (1992) Rapid micropropagation of tea (Camellia spp). J Plant Crops. 20:248–251

    Google Scholar 

  • Rani V., Ajay P., Raina SN. (1995) Random amplified polymorphic DNA (RAPD) markers for genetic analysis in micropropagated plants of Populus deltoides Marsh. Plant Cell Rep. 14: 459–462.

    Google Scholar 

  • Redenbaugh K., Fujii J.A., Slade D. (1991) Synthetic seed technology. In: Vasil K.I. (ed) Scale-up and automation in plant propagation. Cell culture and somatic cell genetics of plants, Vol. 8. Academic Press Inc., New York, pp. 35–74.

    Google Scholar 

  • Redenbaugh K., Slade D., Viss P., Fujii J.A. (1987) Encapsulation of somatic embryos in synthetic seed coats. Hort Sci 22, 803–809.

    Google Scholar 

  • Roberts D.R., Sutton B.C.S., Flinn B.S. (1990) Synchronous and high frequency germination of interior spruce somatic embryo following partial drying at high relative humidity. Can. J. Bot. 68: 1086–1093.

    Article  Google Scholar 

  • Sandal I., Bhattacharya A., Ahuja P.S. (2001) An efficient liquid culture system for tea shoot proliferation. Plant Cell, Tissue Organ Culture. 65: 75–80.

    Article  CAS  Google Scholar 

  • Sarathchandra TM., Upali P.D., Wijeweardena R.G.A. (1988) Studies on the tissue culture of tea (Camellia sinensis (L.) O. Kuntze) 4. Somatic embryogenesis in stem and leaf callus cultures. Sri Lank. J. Tea Sci. 52: 50–54.

    Google Scholar 

  • Samar M. (1985) Callus formation from explanted organs of tea (Camellia sinensis L.). J. Tea Sci. 54: 18–22.

    Google Scholar 

  • Scowcroft W.R. (1984) Genetic variability in tissue culture: Impact on germplasm conservation and utilization. Report on the IBPGR, Rome, p.152.

    Google Scholar 

  • Scowcroft W.R., Larkin P.J. (1982). Somaclonal variation — a new option for plant improvement. In: Vasil K., Scowcroft W.R., Frey K.J. (eds) Plant improvement and somatic cell genetics, Academic Press, New York, pp. 157–178.

    Google Scholar 

  • Sealy J.R.(1958)A revision of the genus Camellia. R.Ionic Soc. London.

    Google Scholar 

  • Sharma M., Sood A., Nagar P.K., Prakash O., Ahuja P.S. (1999) Direct rooting and hardening of tea microshoots in the field. Plant Cell Tissue Org Cult. 58: 111–118.

    Article  Google Scholar 

  • Shibata M., Kuranuki Y. (1993) Improvement of medium components for in vitro cuttings of tea plant. 1. Effects of concentration of some components of MS medium and comparison between MS medium and woody plant medium. J. Tea Sci. 77: 39–45.

    Google Scholar 

  • Singh A., Sharma J., Rexer K-H., Vanna A. (2000) Plant productivity determinants beyond minerals, water and light: Piriformospora indica- A revolutionary plant growth promoting fungus. Curr. Sci. 79 (11): 1548–1554.

    Google Scholar 

  • Singh I.D. (1980) Non conventional approaches to the breeding of tea in north-east India. Two and a bud. 27: 3–6.

    Google Scholar 

  • Smith M.K. (1998). A review of factors influencing the genetic stability of micropropagated banana fruits. Australian J. Expt. Agric. 43: 219–223.

    CAS  Google Scholar 

  • Sood A., Palni L.M.S., Sharma M., Rao D.V., Chand G., Jain N.K. (1993) Micropropagation of tea using cotyledon culture and encapsulated somatic embryos. J. Plant. Crops. 21: 295–300.

    Google Scholar 

  • Tahardi J.S., Shu W. (1992) Commercialization of clonal micropropagation of superior tea genotypes using tissue culture technology USA-ID/CDR Network meeting on tea crop biotech Costa Rica.

    Google Scholar 

  • Thakor, B.H. (2000). A re-examination of the phylogenetis relationships within the genus Camellia.lot Camellia J. 2000. 130–134.

    Google Scholar 

  • Tian-Ling. (1982) Regeneration of plantlets in cultures of immature cotyledons and young embryos of Camellia oleifera Abel. Acta, Biol. Exp. Sin., 15: 393–403.

    Google Scholar 

  • Tremblay M. M., Tremblay F.M. (1995) Maturation of black spruce somatic embryos: sucrose hydrolysis and resulting osmotic pressure of the medium. Plant Cell Tiss. Org. Cult. 42: 39–46.

    CAS  Google Scholar 

  • Tubbs F. R. (1932). A note on vegetative propagation of tea. Tea Quarterly, 5, 154–156.

    Google Scholar 

  • Tulecke W. (1987) Somatic embryogenesis in woody perennials. In: Bonga J.M., Durian D.J. (eds) Cell and Tissue Culture in Forestry. Vol. 2. Martinus Nijhoff Publishers, Dordrecht, pp. 61–69.

    Chapter  Google Scholar 

  • Tu nstall A. C. (1931) A note of propagation of green shoot cuttings. Quarterly J. ITA, 49–51.

    Google Scholar 

  • Vieitez A.M. (1994) Somatic embryogenesis in Camellia spp. In: Jain S.M., Gupta PK., Newton RJ. (eds) Somatic Embryogenesis in Woody Plants, Kluwer Academic Publishers, The Netherlands, pp. 235–276.

    Google Scholar 

  • Vieitez A.M., Vieitez M.L.. Ballester A., Vieitez E. (1992) Micropropagation of Camellia spp. In: Bajaj Y.P.S. (ed) Biotechnology in Agriculture and Forestry, Vol. 19. High Tech and Micropropagation III. Springer-Verlag, Berlin Heidelberg. pp 361–387

    Chapter  Google Scholar 

  • Vuylsteke D., Swennen R., Wilson G.F., Langhe E.D. (1988) Phenotypic variation among in vitro propagated platain (Musa sp. Cultivar ‘AAB’) Sci. Hort. 36: 79–80.

    Article  Google Scholar 

  • Wachira F., Ogado J. (1995) In vitro regeneration of Camellia sinensis (L.) O. Kuntze by somatic embryo. Plant Cell Rep. 14: 463–466.

    Article  CAS  Google Scholar 

  • Wealth of India. (1950) A directory of Indian raw materials and industrial products. In: Bhatnagar S.S. Chairman, Editorial Committee, Council of Scientific and Industrial Res, Vol. II. New Delhi, India, pp. 26–51.

    Google Scholar 

  • Webster F.B., Roberts D.R., Mclnnis S.M., Sutton B.C.S. (1990) Propagation of interior spruce by somatic embryogenesis. Can. J. For. Res. 20: 1757–1762.

    Article  Google Scholar 

  • Wight W. (1959) Nomenclature and classification of tea plant. Nature. 183: 1726–1728.

    Article  Google Scholar 

  • Wood D.J., Barua D.N. (1958) Species hybrids of tea. Nature. 181: 1674–1675.

    Article  Google Scholar 

  • Wu C.T., Huang T.K., Chen G.R., Chen S.Y. (1981) A review on the tissue culture of tea plants and on the utilization of callus derived plantlets In: Rao A.N. (ed) Tissue culture of Economically Important plants, Proc. Costed. Symp Singapore, pp.104–106.

    Google Scholar 

  • Yan M.Q., Ping C. (1983) Studies on development of embryoids from the culture cotyledons of Thea sinensis L. Sci. Silv. Sin. 19: 25–29.

    Google Scholar 

  • Zhan Z, Ke N., Chen B. (1987) The cytology of tea clonal cultivars fujian shuixian and their infertile mechanism. Proc of Intl Tea quality. Human Health Symp, China, pp. 46.

    Google Scholar 

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Authors and Affiliations

  1. Center for Advance Study in Tea Science and Technology, Department of Plantation Crops and Processing, Uttar Banga Krishi Viswavidyalaya, P.O: Pundibari, Dist Cooch Behar, West Bengal, 736165, India

    Tapan Kumar Mondal

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  1. FAO/IAEA Joint Division, International Atomic Agency, Vienna, Austria

    S. Mohan Jain

  2. Forestry and Forest Products Research Institute, Tsukuba, Japan

    Katsuaki Ishii

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Mondal, T.K. (2003). Micropropagation of tea (Camellia sinensis L.). In: Jain, S.M., Ishii, K. (eds) Micropropagation of Woody Trees and Fruits. Forestry Sciences, vol 75. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-0125-0_23

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  • DOI: https://doi.org/10.1007/978-94-010-0125-0_23

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