Development of a Biphasic Culture Process for Recombinant Protein Production in Human CAP Cells

Abstract

In order to evaluate physical parameters for batch cultivation of stable human CAP cell lines, a biphasic batch process with pH-shift was established. Importantly, the biphasic process resulted in a significant increase of volumetric productivity of a highly complex and fully glycosylated protein. To achieve high product yields of recombinant protein in stable cell lines, increased growth rate and high productivity are needed. Low levels of inhibitory metabolic products and sufficient substrate supply are known to lead to extended process time, increased volumetric productivity and optimized product quality.