Abstract
Circulating hemocytes of the solitary protochordates do not survive for prolonged periods in vitro. Subsequently, pharyngeal tissue, which is considered a hemocytopoietic tissue, has been cultured by Raftos et al. (1990). The following procedure is based on their method.
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References
Raftos, D.A., Stillman, D.L. and Cooper, E.L (1990) In vitro culture of tissue from the tunicate Styela clava. In Vitro Cell. Dev. Biol. 26, 962–970.
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© 2002 Springer Japan
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Mitsuhashi, J. (2002). Prochordata. In: Invertebrate Tissue Culture Methods. Springer Lab Manual. Springer, Tokyo. https://doi.org/10.1007/978-4-431-67875-5_13
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DOI: https://doi.org/10.1007/978-4-431-67875-5_13
Publisher Name: Springer, Tokyo
Print ISBN: 978-4-431-70313-6
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