Abstract
Measurement of the adenosine A1 receptor (A1-R) with positron emission tomography (PET) using a newly developed positron ligand, [1-methyl-11C]8-dicyclopropylmethyl-1-methyl-3-propylxanthine (MPDX), were performed in a cat middle cerebral artery (MCA) occlusion and reperfusion. Eighteen adult cats underwent PET measurement of, 1) cerebral blood flow (CBF), 2) A1-R, 3) central benzodiazepine receptor (BDZ-R) and 4) glucose metabolism with 15O labeled water, MPDX, 11C-flumazenil (FMZ) and 18F-fluorodeoxyglucose (FDG), respectively. The CBF, A1-R, BDZ-R and FDG uptake were serially measured after 60 min occlusion of MCA in this order. MPDX binding and FMZ binding, but not CBF and FDG uptake, were significantly reduced in the groups with severer ischemic insult than in the groups with no or milder insults. Of the two receptor ligands, the reduction rate of the MPDX binding to A1-Rs was larger in a group that caused fatal ischemic insult. The newly developed PET in vivo imaging technique using MPDX was suitable in evaluating the function of adenosine and A1-Rs in relation to cerebral ischemia.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Ardekani BA, Braun M, Hutton BF, Kanno I, Iida H (1995) A fully automatic multimodality image registration algorithm. J Comput Assist Tomogr 19: 615–623
Dunwiddie TV (1999) Adenosine and suppression of seizures. Adv Neurol 79: 1001–1010
Furuta R, Ishiwata K, Kiyosawa M, Ishii S, Saito N, Shimada J, Endo K, Suzuki F, Senda M (1996) Carbon-11-Iabeled KF15372: a potential central nervous system adenosine A1 receptor ligand. J Nucl Med 37: 1203–1207
Hagberg H, Andersson P, Lacarewicz J, Jacobson I, Butcher S, Sandberg M (1987) Extracellular adenosine, inosine, hypoxanthine, and xanthine in relation to tissue nucleotides and purines in rat striatum during transient ischemia. J Neurochem 49: 227–231
Headrick JP, Bendall MR, Faden AI, Vink R (1994) Dissociation of adenosine levels from bioenergetic state in experimental brain trauma: potential role in secondary injury. J Cereb Blood Flow Metab 14: 853–861
Heiss WD, Graf R, Fujita T, Ohta K, Bauer B, Lottgen J, Wienhard K (1997) Early detection of irreversibly damaged ischemic tissue by flumazenil positron emission tomography in cats. Stroke 28: 2045–2051; discussion 2051–2052
Heiss WD, Kracht L, Grond M, Rudolf J, Bauer B, Wienhard K, Pawlik G (2000) Early [(11)C]Flumazenil/H(2)O positron emission tomography predicts irreversible ischemic cortical damage in stroke patients receiving acute thrombolytic therapy. Stroke 31: 366–369
Ishiwata K, Nariai T, Kimura Y, Oda K, Kawamura K, Ishii K, Senda M, Wakabayashi S, Shimada J (2002) Preclinical studies on [11C]MPDX for mapping adenosine AI receptors by positron emission tomography. Ann Nucl Med 16: 377–382
Ishiwata K, Noguchi J, Wakabayashi S, Shimada J, Ogi N, Nariai T, Tanaka A, Endo K, Suzuki F, Senda M (2000) 11C-labeled KF18446: a potential central nervous system adenosine A2a receptor ligand [in process citation]. J Nucl Med 41: 345–354
Ishiwata K, Takai H, Nonaka H, Ishii S, Simada J, Senda M (2001) Synthesis and preliminary evaluation of a carbon-11-labeled adenosin e tran sport er blocker [(11)C]KF21562. Nucl Med Biol 28: 281–285
Matsumoto K, Graf R, RosnerG, Shimada N, Heiss WO (1992) Flow thresholds for extracellular purine catabolite elevation in cat focal ischemia. Brain Res 579: 309–314
Melani A, Pantoni L, Corsi C, Bianchi L, Monopoli A, Bertorelli R, Pepeu G, Pedata F (1999) Striatal outflow of adenosine, excitatory amino acids, gamma-aminobutyric acid, and taurine in awake freely moving rats after middle cerebral artery occlusion: correlations with neurological deficit and histopathological damage. Stroke 30: 2448–2454; discussion 2455
Nilsson P, Hillered L, Ponten U, Ungerstedt U (1990) Changes in cortical extracellular levels of energy-related metabolites and amino acids following concussive brain injury in rats. J Cereb Blood Flow Metab 10: 631–637
Noguchi J, Ishiwata K, Furuta R, Simada J, Kiyosawa M, Ishii S, Endo K, Suzuki F, Senda M (1997) Evaluation of carbon-11 labeled KF15372 and its ethyl and methyl derivatives as a potential CNS adenosine A1 receptor ligand. Nucl Med Biol 24: 53–59
Noguchi J, Ishiwata K, Wakabayashi S, Nariai T, Shumiya S, Ishii S, Toyama H, Endo K, Suzuki F, Senda M (1998) Evaluation of carbon-11-labeled KF17837: a potential CNS adenosine A2a receptor ligand. J Nucl Med 39: 498–503
Rudolphi KA, Schubert P, Parkinson FE, Fredholm BB (1992) Adenosine and brain ischemia. Cerebrovasc Brain Metab Rev 4: 346–369
Rudolphi KA, Schubert P, Parkinson FE, Fredholm BB (1992) Neuroprotective role of adenosine in cerebral ischaemia. Trends Pharmacol Sci 13: 439–445
Shimada Y, Ishiwata K, Kiyosawa M, Nariai T, Oda K, Toyama H, Suzuki F, Ono K, Senda M (2002) Mapping adenosine A(1) receptors in the cat brain by positron emission tomography with [11C]MPDX. Nucl Med Biol 29: 29–37
Shimada Y, Uemura K, Ardekani BA, Nagaoka T, Ishiwata K, Toyama H, Ono K, Senda M (2000) Application of PET-MRI registration techniques to cat brain imaging. J Neurosci Methods 101: 1–7
Wakabayashi S, Nariai T, Ishiwata K, Nagaoka T, Hirakawa K, Oda K, Sakiyama Y, Shumiya S, Toyama H, Suzuki F, Senda M (2000) A pet study of adenosine A1 receptor in anesthetized monkey brain [In Process Citation]. Nucl Med Biol 27: 401–406
Winn HR, Welsh JE, Rubio R, Berne RM (1980) Changes in brain adenosine during bicuculline-induced seizures in rats. Effects of hypoxia and altered systemic blood pressure. Circ Res 47: 568–577
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2003 Springer-Verlag Wien
About this paper
Cite this paper
Nariai, T. et al. (2003). PET neuroreceptor imaging as predictor of severe cerebral ischemic insult. In: Kuroiwa, T., et al. Brain Edema XII. Acta Neurochirurgica Supplements, vol 86. Springer, Vienna. https://doi.org/10.1007/978-3-7091-0651-8_10
Download citation
DOI: https://doi.org/10.1007/978-3-7091-0651-8_10
Publisher Name: Springer, Vienna
Print ISBN: 978-3-7091-7220-9
Online ISBN: 978-3-7091-0651-8
eBook Packages: Springer Book Archive