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Automation of Cytofluorometry by Use of the Impulsmicrophotometer

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Fluorescence Techniques in Cell Biology

Abstract

For the automation of microscope fluorometry, two different principles can be applied. First, it is possible to measure fluorescent particles deposited on slides using a scanning microscope photometer. Secondly, chemical and physicochemical properties of fluorescent particles can be measured by means of an automatic flow-through device. For studying the proliferation kinetics of cells exposed to physical (e.g., ionizing or nonionizing radiation) or chemical treatment (e.g., cytostatica), flow-through methods are very suitable because of their high measuring speed. The same is true for cytopathological problems.

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Göhde, W. (1973). Automation of Cytofluorometry by Use of the Impulsmicrophotometer. In: Thaer, A.A., Sernetz, M. (eds) Fluorescence Techniques in Cell Biology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-49204-4_9

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  • DOI: https://doi.org/10.1007/978-3-642-49204-4_9

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-49206-8

  • Online ISBN: 978-3-642-49204-4

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