Abstract
Various reactions that produce color are known to be selective for certain types of carbohydrates. It was difficult to identify N-acetyl-glucosamine in hyaluronan because the hyaluronan was produced very low in microorgansims. Besides, in the traditional methods of Morgan-Elson, using alkali borate to lysis hyaluronan to give N-acety-D-glucosamine caused the unspecific products that could not react to di-methyl-benzaldehyde (DMAB). The study developed the method to detect N-acetyl-D-glucosamine by thin layer chromatography (TLC) that showed the stable purple traces on TLC than in liquid. The study was confirmed by applying high pressure liquid chromatography (HPLC).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2013 IFMBE
About this paper
Cite this paper
Tu, N.H.K., Thien, P.V.M. (2013). Detection of N-Acetyl-D-Glucosamine in Hyaluronan by Thin Layer Chromatography. In: Toi, V., Toan, N., Dang Khoa, T., Lien Phuong, T. (eds) 4th International Conference on Biomedical Engineering in Vietnam. IFMBE Proceedings, vol 49. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-32183-2_46
Download citation
DOI: https://doi.org/10.1007/978-3-642-32183-2_46
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-32182-5
Online ISBN: 978-3-642-32183-2
eBook Packages: EngineeringEngineering (R0)