Summary
Monitoring and evaluation of the dynamic of stem cells growth in culture is important in the regenerative medicine as a tool for cells population increasing to the size needed to therapeutic bprocedure. In this paper the automatic segmentation method of cells images from bright field microscope is proposed. It is based on the textural segmentation and morphological watershed. Textural segmentation aims at detecting within the image regions with intensive textural features, which refer to cells. Texture features are detected using local mean absolute deviation measure. Final, precise segmentation is achieved by means of morphological watershed on the gradient image modified by the imposition of minima derived from the result of rough segmentation. The proposed scheme can be applied to segment other images containing object characterized by their texture located on the uniform background.
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References
Alberts, B., Bray, D., Lewis, J., Raff, M., Roberts, K., Watson, J.D.: Molecular Biology of the Cell, 3rd edn. Garland Publishing Inc., New York (1994)
Bellet, F., Salotti, J.M., Garbay, C.: Une approche opportunists et cooperative pour la vision de bas niveau. Traitement du Signal 12(5), 479–494 (1995)
Boucher, A., Doisy, A., Ronot, X., Garbay, C.: Cell Migration Analysis After in Vitro Wounding Injury with a Multi-Agent Approach. Artificial Inetligence Review 12, 137–162 (1998)
Boier Marti, I.M., Martineus, D.C., et al.: Identification of spherical virus particles in digitized images of entire electron micrographs. Journal of Structural Biology 120, 146–157 (2005)
Buzanska, L., Jurga, M., Stachowiak, E.K., Stachowiak, M.K., Domanska-Janik, K.: Stem Cell and Development 15, 391–406 (2006)
Cocquerez, J.P., Philipp, S.: Analyse d’images: filtrage et segmentation. Masson (1995)
Comaniciu, D., Meer, P.: Cell image segmentation for diagnostic pathology. In: Suri, J.S., Setarehdan, S.K., Singh, S. (eds.) Advanced algorithmic approaches to medical image segmentation: state-of-the-art application in cardiology, neurology, mammography and pathology, pp. 541–558 (2001)
Frank, J., Radermacher, M., et al.: Spider and web: Processing and visualization of images in 3D electron microscopy and related fields. Journal of Structural Biology 116, 190–199 (1996)
Goldman, R.D., Spector, D.L.: Live cell Imaging. A laboratory Manual. CSHL Press, New York (2005)
Iwanowski, M.: Binary Shape Characterization Using Morphological Boundary Class Discrimnation Fnctions. In: Kurzynski, M., Puchala, E., Wozniak, M., Zolnierek, A. (eds.) Computer Recognition Systems, pp. 303–312. Springer, Heidelberg (2007)
Jiang, K., Liao, Q.M., Dai, S.Y.: A novel white blood cell segmentation scheme using scale-space ltering and watershed clustering. In: Proc. Int. Conf. on Machine Learning and Cybernetics, vol. 5, pp. 2820–2825 (2003)
Kivioja, T., Ravantti, J., et al.: Local avarage intensty-based method for identifying spherical particles in electron micrographs. Journal of Structural Biology 131, 126–134 (2000)
Korzynska, A., Strojny, W., Hoppe, A., Wertheim, D., Hoser, P.: Segmentation of microscope images of living cells. Pattern Anal. Applic. 10, 301–319 (2007)
Korzynska, A.: Automatic Counting of Neural Stem Cells Growing in Cultures. In: Kurzynski, M., Puchala, E., Wozniak, M., Zolnierek, A. (eds.) Computer Recognition Systems, pp. 604–612. Springer, Heidelberg (2007)
Korzyñska, A., Iwanowski, M.: Detection of Mitotic Cell Fraction in Neural Setem Cells in Culture. In: Pietka, E., Kawa, J. (eds.) Information Technology in Biomedicine. Advances in Soft Computing, vol. 47, pp. 365–376. Springer, Heidelberg (2008)
Korzyñska, A., Dobrowolska, E., Zychowicz, M., Hoser, P.: Examination of the microscopic imaging of the neural stem cells for counting a number of cells. Abstracts of 9th International Symposium: Molecular basis of pathology and therapy in neurological disorders, p. 66 (2008)
Ludtke, J., Baldwin, P., Chiu, W.: EMAN: Semiautomared software for high-resolution signal-particle reconstruction. J. of Struct. Biol. 128, 82–97 (1999)
Miroslaw, L., Chorazyczewski, A., Buchholz, F., Kittler, R.: Correlation-based Method for Automatic Mitotic Cell Detection in Phase Contrast Microscopy. In: Kurzynski, M., Puchala, E., Wozniak, M., Zolnierek, A. (eds.) Computer Recognition Systems, pp. 627–634. Springer, Heidelberg (2005)
Nicholson, W.V., Glaeser, R.M.: Review: Automatic particle detection in electron microscopy. Journal of Structural Biology 133, 90–101 (2001)
Periasamy, A.: Methods in Cellular Imaging. Oxford University Press, Oxford (2001)
Serra, J., Vincent, L.: An overview of morphological filtering. Circuit systems Signal Processing 11(1) (1992)
Serra, J.: Image analysis and mathematical morphology, vol. 1. Academic Press, London (1983)
Serra, J.: Image analysis and mathematical morphology, vol. 2. Academic Press, London (1988)
Smereka, M.: Detection of ellipsoidal shapes using contour grouping. In: Kurzynski, M., Puchala, E., Wozniak, M., Zolnierek, A. (eds.) Computer Recognition Systems, pp. 443–450. Springer, Heidelberg (2005)
Sinha, N., Ramakrishnan, A.G.: Automation of differential blood count. In: Proc. Conf. on Convergent Technologies for Asia-Pacific Region, vol. 2, pp. 547–551 (2003)
Soille, P.: Morphological image analysis. Springer, Heidelberg (2002)
Vincent, L.: Morphological Grayscale Reconstruction in Image Analysis: Applications and Efficient Algorithms. IEEE Trans. on Image Processing 2(2) (1993)
Yogesan, K., Jorgensen, T., Albregtsen, F., et al.: Cytometry 24, 268–276 (1996)
Zama, N., Katow, H.: A method of quantitative analysis of cell migration using a computerized time-lapse videomicroscopy. Zool. Sci. 5, 53–60 (1988)
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Iwanowski, M., Korzynska, A. (2009). Detection of the Area Covered by Neural Stem Cells in Cultures Using Textural Segmentation and Morphological Watershed. In: Kurzynski, M., Wozniak, M. (eds) Computer Recognition Systems 3. Advances in Intelligent and Soft Computing, vol 57. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-93905-4_64
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DOI: https://doi.org/10.1007/978-3-540-93905-4_64
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