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Amino Acid Analysis and Sequencing — What is State-of-the-Art?

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Methods in Protein Sequence Analysis

Part of the book series: Advances in Life Sciences ((ALS))

Summary

Based on analyses and surveys completed by as many as 112 protein chemistry laboratories, an average of nearly 100 picomoles of protein is needed to sequence the first 15 or more residues while a total of about 4 μg protein is sufficient to carry out a triplicate hydrolysis/analysis that will give an amino acid composition with about 86% compositional accuracy. On average, approximately 700 picomoles protein is required in order to isolate and sequence a 15 residue tryptic peptide.

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Author information

Authors and Affiliations

  1. Univ. of Wisconsin Biotechnology Center, Madison, WI, 53705, USA

    Ronald L. Niece

  2. Dept. of Biochemistry, Univ. of Washington, Seattle, WA, 98915, USA

    Lowell H. Ericsson

  3. Dept. of Biological Chemistry, UCLA, Los Angeles, CA, 90024, USA

    Audree V. Fowler

  4. Beckman Center, Stanford University, Stanford, CA, 94305, USA

    Alan J. Smith

  5. Wistar Institute, Philadelphia, PA, 19104, USA

    David W. Speicher

  6. W. Alton Jones Cell Science Center, Lake Placid, NY, 12946, USA

    John W. Crabb

  7. Yale University Protein and Nucleic Acid Chemistry Facility, New Haven, CT, 06510, USA

    Kenneth R. Williams

Authors
  1. Ronald L. Niece
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  2. Lowell H. Ericsson
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  3. Audree V. Fowler
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  4. Alan J. Smith
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  5. David W. Speicher
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  6. John W. Crabb
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  7. Kenneth R. Williams
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Editor information

Editors and Affiliations

  1. Department of Chemistry I, Karolinska Institute, S-104 01, Stockholm, Sweden

    Hans Jörnvall , Jan-Olov Höög  & Ann-Margreth Gustavsson ,  & 

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© 1991 Springer Basel AG

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Niece, R.L. et al. (1991). Amino Acid Analysis and Sequencing — What is State-of-the-Art?. In: Jörnvall, H., Höög, JO., Gustavsson, AM. (eds) Methods in Protein Sequence Analysis. Advances in Life Sciences. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-5678-2_12

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  • DOI: https://doi.org/10.1007/978-3-0348-5678-2_12

  • Publisher Name: Birkhäuser, Basel

  • Print ISBN: 978-3-0348-5680-5

  • Online ISBN: 978-3-0348-5678-2

  • eBook Packages: Springer Book Archive

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