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Flow Cytometry: Instrumentation, Applications, Future Trends and Limitations

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Standardization and Quality Assurance in Fluorescence Measurements II

Part of the book series: Springer Series on Fluorescence ((SS FLUOR,volume 6))

Abstract

Flow cytometry is the measurement of cells or other particles flowing past one or more sensing zones. Most commonly the sensing zone is a focused beam of light, and particles are characterized by the scattered and fluorescent light pulses that are created as the particle passes through the light beam. Unlike microscopic image analysis, flow cytometry generally measures the total signal from a particle and does not provide morphological detail on the shape or size of the sources of signal within the particle. The strength of flow cytometry is rapidly providing measurements from multiple fluorochrome stains along with several intrinsic measures of particle characteristics. In some flow cytometers particles with selected measurement properties can be physically sorted and collected. This chapter provides an overview of the technology and application of flow cytometry. All major aspects of the technology are introduced, and references to original literature are provided for readers interested in additional details. Special attention is given the use of fluorescence measurements.

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Authors and Affiliations

  1. BD Biosciences, 2350 Qume Drive, 95131, San Jose, CA, USA

    Robert A. Hoffman

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  1. Robert A. Hoffman
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Correspondence to Robert A. Hoffman .

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Ute Resch-Genger

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Hoffman, R.A. (2008). Flow Cytometry: Instrumentation, Applications, Future Trends and Limitations. In: Resch-Genger, U. (eds) Standardization and Quality Assurance in Fluorescence Measurements II. Springer Series on Fluorescence, vol 6. Springer, Berlin, Heidelberg. https://doi.org/10.1007/4243_2008_037

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