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Tunable Promoters in Synthetic and Systems Biology

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Reprogramming Microbial Metabolic Pathways

Part of the book series: Subcellular Biochemistry ((SCBI,volume 64))

Abstract

Synthetic and systems biologists need standardized, modular and orthogonal tools yielding predictable functions in vivo. In systems biology such tools are needed to quantitatively analyze the behavior of biological systems while the efficient engineering of artificial gene networks is central in synthetic biology. A number of tools exist to manipulate the steps in between gene sequence and functional protein in living cells, but out of these the most straight-forward approach is to alter the gene expression level by manipulating the promoter sequence. Some of the promoter tuning tools available for accomplishing such altered gene expression levels are discussed here along with examples of their use, and ideas for new tools are described. The road ahead looks very promising for synthetic and systems biologists as tools to achieve just about anything in terms of tuning and timing multiple gene expression levels using libraries of synthetic promoters now exist.

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Abbreviations

ATc:

anhydrotetracycline

DXP:

1-deoxy-D-xylulose-5-phosphate

EGFP:

enhanced green fluorescent protein

GFP:

green fluorescent protein

GOI:

gene of interest

gTME:

global transcription machinery engineering

GusA:

β-glucuronidase

KO:

knock-out

MAGE:

multiplex automated genome engineering

MCA:

metabolic control analysis

ORF:

open reading frame

Pfk:

phosphofructokinase

PLS-R:

partial least squares regression

PSP:

promoter strength predictive

RBS:

ribosome binding site

SOE PCR:

splicing by overlap extension PCR

SPL:

synthetic promoter library

TAL:

transcription activator-like

TALOR:

transcription activator-like orthogonal repressor

yEGFP:

yeast enhanced green fluorescent protein

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Authors and Affiliations

  1. Center for Systems Microbiology, Department of Systems Biology, Technical University of Denmark, Matematiktorvet 301/242, 2800, Lyngby, Denmark

    Tore Dehli, Christian Solem & Peter Ruhdal Jensen

Authors
  1. Tore Dehli
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  2. Christian Solem
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  3. Peter Ruhdal Jensen
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Correspondence to Peter Ruhdal Jensen .

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Editors and Affiliations

  1. , State Key Laboratory, Jiangnan University, Lihu Avenue 1800, Wuxi, 214122, Jiangsu, China, People's Republic

    Xiaoyuan Wang

  2. School of Biotechnology, Jiangnan University, Lihu Avenue 1800, Wuxi, 214122, China, People's Republic

    Jian Chen

  3. , Div. of Life Sciences, King's College London, Stamford Street 150, London, SE1 9NH, United Kingdom

    Peter Quinn

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Dehli, T., Solem, C., Jensen, P.R. (2012). Tunable Promoters in Synthetic and Systems Biology. In: Wang, X., Chen, J., Quinn, P. (eds) Reprogramming Microbial Metabolic Pathways. Subcellular Biochemistry, vol 64. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-5055-5_9

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