Abstract
We evaluate in rats the role of NO in the solitary tract nucleus (STn) after an anoxic stimulus to carotid body chemoreceptor cells (CChrc) with cyanide (NaCN), on the hyperglycemic reflex with glucose retention by the brain (BGR) and FOS expression (FOS-ir) in the STn. The results suggest that nitroxidergic pathways in the STn may play an important role in glucose homeostasis. A NO donor such as sodium nitroprusside (NPS) in the STn before CChrc stimulation increased arterial glucose level and significantly decreased BGR. NPS also induced a higher FOS-ir expression in STn neurons when compared to neurons in control rats that only received artificial cerebrospinal fluid (aCSF) before CChrc stimulation. In contrast, a selective NOS inhibitor such as Nω-nitro-L-arginine methyl ester (L-NAME) in the STn before CChrc stimulation resulted in an increase of both, systemic glucose and BGR above control values. In this case, the number of FOS-ir positive neurons in the STn decreased when compared to control or to NPS experiments. FOS-ir expression in brainstem cells suggests that CChrc stimulation activates nitroxidergic pathways in the STn to regulate peripheral and central glucose homeostasis. The study of these functionally defined cells will be important to understand brain glucose homeostasis.
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References
Alcayaga, J., Barrios, M., Bustos, F., Miranda, G., Molina, M.J. & Iturriaga, R. 1999, Modulatory effect of nitric oxide on acetylcholine-induced activation of cat petrosal ganglion neurons in vitro, Brain Res. 825, 194–198.
Almeida, A., Cidad, P., Delgado-Esteban, M., Fernández, E., García-Nogales, P. & Bolaños J.P. 2005, Inhibition of mitochondrial respiration by nitric oxide: its role in glucose metabolism and neuroprotection, J Neurosci Res 79: 166–171
Álvarez-Buylla, R. & Álvarez-Buylla, E. 1988, Carotid sinus receptors participate in glucose homeostasis, Respir Physiol, 72: 347–360.
Álvarez-Buylla, R. & Roces de Álvarez-Buylla, E. 1975, Hypoglycemic conditioned reflex in rats: preliminary study of its mechanism, J Comp Physiol Psychol, 88: 155–160.
Brodsky, S.V., Gao, S., Li, H. & Goligorsky, M.S. 2002, Hyperglycemic switch from mitochondrial nitric oxide to superoxide production in endothelial cells, Am. J. Physiol Hear Cir Physiol, 283: H2130–H2139.
Chan, S.H., Chang, K.F., Ou, C.C. & Chan, J.Y. 2004. Nitric oxide regulates c-fos expression in nucleus tractus solitarii induced by baroreceptor activation via cGMP-dependent protein kinase and cAMP response element-binding protein phosphorylation. Molec Pharmacol, 65, 319–25.
Dias, A.C., Vitela, M., Colombari, E. & Mifflin, S.W. 2005, Nitric oxide modulation of glutamatergic baroreflex, and cardiopulmonary transmission in the nucleus of the solitary tract, Amer J Physiol: Heart Cir Physiol, 288: H256–H262.
Housley, G.D. & Sinclair, J.D. 1988, Localization by kainic acid lesions of neurons P transmitting the carotid chemoreceptor stimulus for respiration in rat, J Physiol, 406: 99–114.
Iturriaga, R., Villanueva, S. & Mosqueira, M. 2000, Dual effects of nitric oxide on cat carotid body chemoreception, J Appl Physiol, 89: 1005–1012.
Kadowaki, K., Kishimoto, J., Leng, G. & Emson, P.C. 1994, Up-regulation of nitric oxide synthase (NOS) gene expression together with NOS activity in the rat hypothalamo-hypophyseal system after chronic salt loading: evidence of a neuromodulatory role of nitri oxide in arginine vasopressin and oxytocin secretion, Endocrinol, 134: 1011–1017.
Kobelt, P., Tebbe, J.J., Tjandra, I., Bae, H.G., Ruter, J., Klapp, B.F., Wiedenmann, B. & Monnikes, H. 2004, Two immunocytochemical protocols for immunofluorescent detection of c-Fos positive neurons in the rat brain, Brain Res Prot, 13: 45–52.
López-Barneo, J. 2003, Oxygen and glucose sensing by carotid body glomus cells, Curr Op Neurobiol, 13: 493–9.
Mifflin, S.W. 1996, Convergent carotid sinus nerve and superior laryngeal nerve afferent inputs to neurons in the NTS, Amer J Physiol, 271: R870–R880.
Moncada, S., Palmer, R.M. & Higgs, E.A. 1991, Nitric oxide: physiology, pathophysiology and pharmacology, Pharmacol Rev, 43: 109–142.
Montero, S., Cadenas, J.L., Lemus, M., Álvarez- Buylla, E. & Álvarez- Buylla, R. 2006, Nitric oxide in brain glucose retention after carotid body receptors stimulation with cyanide in rats, Adv Exp Med Biol, 580: 293–300.
Paxinos, G. & Watson, C. 1986, The rat brain in stereotactic coordinates, Academic Press, San. Diego.
Prabhakar, N.R. 1994, Neurotransmitters in the carotid body, Adv Exp Med Biol, 360: 57–69.
Sugimoto, Y., Yamada, J., Yoshikawa, T. & Horisaka, K. 1997, Inhibitory effects of nitric oxide synthase inhibitor, N(G)-nitro-L-arginine methyl ester (L-NAME), on 2-deoxy-D-glucose-induced hyperglycemia in rats, Biol Pharmacol Bull, 20: 1307–1309.
Sunico, C.R., Portillo, F., González-Forero, D. & Moreno-López, B. 2005, Nitric oxide-directed synaptic remodeling in the adult mammal CNS, J Neurosci, 25: 1448–1458.
Talman, W.T., óDragon, D.N., óOhta, H., & óLin, L.H. 2001, Nitroxidergic influences on cardiovascular control by NTS: a link with glutamate, Ann New York Acad Sci, 940: 169–78.
Zhang, M., Buttigieg, J. & Nurse, C.A. 2007, Neurotransmitter mechanisms mediating low-glucose signalling in cocultures and fresh tissue slices of rat carotid body, J Physiol, 578: 735–750.
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Lemus, M., Montero, S., Luquín, S., García, J., De Álvarez-Buylla, E.R. (2009). Nitric Oxide in the Solitary Tract Nucleus (STn) Modulates Glucose Homeostasis and FOS-ir Expression After Carotid Chemoreceptor Stimulation. In: Gonzalez, C., Nurse, C.A., Peers, C. (eds) Arterial Chemoreceptors. Advances in Experimental Medicine and Biology, vol 648. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-2259-2_46
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DOI: https://doi.org/10.1007/978-90-481-2259-2_46
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