Skip to main content
SpringerLink
Log in

Myelin Basic Protein, the “Executive” Molecule of the Myelin Membrane

  • Chapter
Cell Biology and Pathology of Myelin

Part of the book series: Altschul Symposia Series ((ALSS,volume 4))

Abstract

Myelin basic protein (MBP) has been known since the mid 1960’s. Its primary structure was reported by two groups independently, Eylar et al. reported on the bovine MBP while Carnegie reported on the human MBP (Eylar et al., 1971; Carnegie, 1971). The molecular weight of this protein was computed to be 18500. It is devoid of cysteinyl residues, is highly basic (12 lysyl and 19 arginyl residues) and has 11 prolines, 3 of which occur as a triproline sequence.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Baldwin GS, Carnegie PR (1971): Specific enzyme methylation of an arginine in the experimental allergic encephalomyelitis protein from human myelin. Science 171: 579–581.

    Article  PubMed  CAS  Google Scholar 

  • Bhat NR, Zhang P (1996): Activation of mitogen activated protein kinases in oligodendrocytes. J Neurochem 66: 1986–1994.

    Article  PubMed  CAS  Google Scholar 

  • Boison D, Stoffel W (1994): Disruption of the compact myelin sheath of axons of the central nervous system in proteolipid protein deficient mice. Proc Natl Acad Sci (USA) 91: 11709–11713.

    Article  CAS  Google Scholar 

  • Boulias C, Moscarello MA (1994): ADP-ribosylation of human myelin basic protein. J Neurochem 60: 351–359.

    Google Scholar 

  • Boulias C, Mastronardi FG, Moscarello MA (1995): ADP-ribosyltransferase activity in myelin membranes isolated from human brain. Neurochem Res 20: 1269–1277.

    Article  PubMed  CAS  Google Scholar 

  • Bourre JM, Cloez I, Galliot M, Buisine A, Dumont O, Piciotti M, Prouillet F, Bourdon R (1987): Occurrence of manganese, copper and zinc in myelin. Alterations in the peripheral nervous system of dysmyelinating trembler mutant are at variance with brain mutants (quaking and shiverer). Neurochem Int 10: 281–286.

    Article  PubMed  CAS  Google Scholar 

  • Brady GW, Birnbaum PS, Moscarello MA, Papahadjopoulos D (1979): Liquid diffraction analysis of the model membrane system, egg lecithin and myelin protein (N-2). Biophys J 25: 23–42.

    Article  Google Scholar 

  • Brady GW, Murthy NS, Fein DB, Wood DD, Moscarello MA (1981): The effect of basic myelin protein on multi-layer membrane formation. Biophys J 34: 345–360.

    Article  PubMed  CAS  Google Scholar 

  • Carnegie PR (1971): Amino acid sequence of the encephalitogenic protein of human myelin. Biochem J 123: 57–67.

    PubMed  CAS  Google Scholar 

  • Cavatorta P, Giovanelli S, Bobba A, Riccio P, Szabo AG, Quagliariello E (1994): Myelin basic protein interaction with zinc and phosphate. Fluorescence studies on the water-soluble form of the protein. Biophys J 66: 1174–1179.

    Google Scholar 

  • Chan CK, Ramwani J, Moscarello MA (1988): Myelin basic protein binds GTP at a single site in the N-terminus. Biochem Biophys Res Comm 152: 1468–1473.

    Article  PubMed  CAS  Google Scholar 

  • Chanderkar LP, Park, WK, Kim, S (1986): Studies on myelin-basic-protein methylation during mouse brain development. Biochem J 240: 471–479.

    PubMed  CAS  Google Scholar 

  • Cheifetz S, Moscarello MA (1985): Effect of basic protein charge microheterogeneity on protein-induced aggregation of lipid vesicles containing a mixture of acidic and neutral phospholipids. Biochemistry 24: 1909–1914.

    Article  PubMed  CAS  Google Scholar 

  • Cheifetz S, Boggs JM, Moscarello MA (1985): Increase in vesicle permeability mediated by myelin basic protein: effect of phosphorylation of basic protein. Biochemistry 24:5I70–5175.

    Google Scholar 

  • Cheifetz S, Moscarello MA, Deber CM (1984): NMR investigation of the charge isomers of bovine myelin basic protein. Arch Biochem Biophys 233: 151–160.

    Article  PubMed  CAS  Google Scholar 

  • Chen JK, Schreiber SL (1996): Combinatorial synthesis and multidimensional NMR-spectroscopy: an approach to understanding protein-ligand interactions. Agnew Chemie 34: 953–969.

    Article  Google Scholar 

  • Chou FC-H, Chou JC-H, Shapira R. Kibler RF (1976): Basis of microheterogeneity of myelin basic protein. Coleman JE (1992): Zinc proteins: enzymes. storage, proteins, transcription factors and replication proteins. Ann Rev Biochem 61: 897–946.

    Google Scholar 

  • Deibler GE, Martenson RE, Kramer AL Kies MW, Miyamoto E (1975): The contribution of phosphorylation and loss of carboxyterminal arginine to the microheterogeneity of myelin basic protein. J Biol Chem 250: 7931–7938.

    PubMed  CAS  Google Scholar 

  • Dickinson JP, Jones KM, Aparicio SR, Lumsden CE (1970): Localization of encephalitogenic basic protein in the intraperiod line of lamellar myelin. Nature (Lond) 227: 1133–1134.

    Article  CAS  Google Scholar 

  • Eggens I, Fenderson B, Toyokuni T, Dean B, Stroud M, Hakomori S (1989): Specific interaction between Lex and Lex determinants. A possible basis for cell recognition in preimplantation embryos and in embryonal carcinoma cells. J Biol Chem 264: 9476–84.

    Google Scholar 

  • Eylar EH, Brostoff S, Hashim G, Caccam J, Burnet P (1971): Basic A 1 protein of the myelin membrane, the complete amino acid sequence. J Biol Chem 246: 5770–5784.

    PubMed  CAS  Google Scholar 

  • Golds E, Braun PE (1978): Cross-linking studies on the conformation and dimerization of myelin basic protein. J Biol Chem 253: 8171–8177.

    PubMed  CAS  Google Scholar 

  • Guarnieri M, Himmelstein J. McKhann G (1974): Isolated myelin quantitatively adsorbs antibody to basic protein. Brain Res 72: 172–176.

    Article  PubMed  CAS  Google Scholar 

  • Halliday K (1984): Regional homology in GTP binding proto oncogene products and elongation factors. J Cyc Nuc Res 9: 435–448.

    CAS  Google Scholar 

  • Herndon RW, Rauch HC, Einstein ER (1973): Immunoelectron microscopic localization of the encephalitogenic basic protein in myelin. Immunol Commun 2: 163–172.

    PubMed  CAS  Google Scholar 

  • Inouye H, Kirschner DA (1984): Effects of ZnCl2, on membrane interactions in myelin of normal and shiverer mice. Biochim Biophys Acta 776: 197–208.

    Article  PubMed  CAS  Google Scholar 

  • Kalmholz J, de Ferra F, Prickett C, Lazzarini RA (1986): Identification of three forms of human myelin basic protein by cDNA cloning. Proc Natl Acad Sci (USA) 83: 4962–4966.

    Google Scholar 

  • Kalmholz J. Spielman R, Goglin K (1987): The human myelin basic protein gene: chromosomal localization and RFLP analysis. Am J Hum Genetics 40: 365–373.

    Google Scholar 

  • Kalmholz J. Toffeneti, Lazzarini RA (1988): Organization and expression of the human myelin basic protein gene. J Neurosci Res 21: 62–70.

    Google Scholar 

  • Kojima N, Hakamori S (1991): Cell adhesion, spreading, and motility of GM3-expressing cells based on glycolipid-glycolipid interaction. J Biol Chem 266: 17552–17558.

    PubMed  CAS  Google Scholar 

  • Kojima N, Hakemori S (1989): Specific interaction between gangliotriaosylceramide and sialosyllactosylceramide (GM3) as a basis for specific cellular recognition between lymphoma and melanoma cells. J Biol Chem 264: 20159–20162.

    PubMed  CAS  Google Scholar 

  • Korngurth S. Anderson L (1965): Localization of a basic protein of various species with the aid of fluorescence and electron microscopy. J Cell Biol 26: 157–166.

    Article  Google Scholar 

  • Larocca JN, Ledeen RW, Dworkin B. Makaran MH (1987) Muscarinic receptor binding and muscarinic receptor-mediated inhibition of adenylate cyclase in rat brain myelin. J Neurosci 7: 3869–3876.

    PubMed  CAS  Google Scholar 

  • Li C, Tropak MB, Gerlai R, Clapoff S. Abramow-Newerly W, Trapp B, Paterson A. Roder J (1994): Myelination in the absence of myelin associated glycoprotein. Nature 369: 747–750.

    Article  PubMed  CAS  Google Scholar 

  • Mastronardi FG. Ackerley CA. Arsenault L, Roots BJ, Moscarello MA (1993): Demvelination in a transgenic mouse: a model for multiple sclerosis. J Neurosci Res 36: 315–324.

    Article  PubMed  CAS  Google Scholar 

  • Mastronardi FG, Al-Sabbagh A, Nelson PA, Rego J, Roots BI, Moscarello MA (1996): Myelin basic protein is not affected at the post translational level in chronic EAE mouse brain. J Neurosci Res 44: 344–349.

    Article  PubMed  CAS  Google Scholar 

  • Mastronardi FG, Mak B, Ackerley CA, Roots BI, Moscarello MA (1996): Myelin basic protein changes in DM20 transgenic mice are similar to changes in MBP in multiple sclerosis. J Clin Invest 97: 349–358.

    Article  PubMed  CAS  Google Scholar 

  • McLaurin J, Moscarello MA (1990): The preparation of antibodies reactive against the citrulline-containing charge isomers of myelin basic protein but not against the arginine-containing charge isomer. Anal Biochem 191: 272–277.

    Article  PubMed  CAS  Google Scholar 

  • McLaurin J, Ackerley CA, Moscarello MA (1993): Localization of basic protein in human myelin. J Neurosci Res 35: 618–628.

    Article  PubMed  CAS  Google Scholar 

  • Mendell JR, Whitaker JN (1978): Immunocytochemical localization studies of myelin basic protein. J Cell Biol 76: 502–511.

    Google Scholar 

  • Mendz JL, Barden JA, Martenson RE (1995): Conformation of a tetrapeptide epitope of myelin basic protein. Eur J Biochem 231: 659–666.

    Article  PubMed  CAS  Google Scholar 

  • Mizutani Y, Kamagawa K, Nakanishi K (1989): Effect of urea on hydrophobic interaction: Raman difference spectroscopy on C-H stretching vibration of acetone and C-N stretching vibration of urea. J Phys Chem 93: 5650–5654.

    Google Scholar 

  • Moscarello MA, Pang H, Pace-Asciak CR, Wood DD (1992): The N-terminus of human myelin basic protein consists of C2, C4, C6 and C8 alkycarboxylic acids. J Biol Chem 267: 9779–9782.

    PubMed  CAS  Google Scholar 

  • Moscarello MA, Wood DD, Ackerley C, Boulias C (1994): Myelin in multiple sclerosis is developmentally immature. J Clin Invest 94: 146–154.

    Article  PubMed  CAS  Google Scholar 

  • Napolitano L, LeBaron F, Scaletti J (1967): Preservation of myelin lamellar structure in the absence of lipid. Biophys J 34: 817–826.

    Article  CAS  Google Scholar 

  • Ochoa JL, Porath J, Kempf J, Egly JM (1980): Electron donor-acceptor properties of urea and its role in charge-transfer chromatography.

    Google Scholar 

  • Omlin FX, Webster H de F, Palkovits CE, Cohen SR (1982): Immunocytochemical localization of basic protein in major dense line regions of central and peripheral myelin. J Cell Biol 95: 242–248.

    Google Scholar 

  • Pawson T (1995): Protein modules and signalling networks. Nature 373: 573–580.

    Article  PubMed  CAS  Google Scholar 

  • Poduslo JF, Braun PE (1975): Topographical arrangement of membrane proteins in the intact myelin sheath: Lactoperoxidase incorporation of iodine into myelin surface protein. J Biol Chem 250: 1099–1105.

    Google Scholar 

  • Pribyl TM, Campagnoni CW, Kampf K, Kashima T, Handley VW, McMahon J, Campagnoni AT (1993): The human myelin basic protein gene is included within a 179 kilobase transcription unit: expression in the immune and central nervous system. Proc Natl Acad Sci (USA) 90: 10695–10699.

    Article  CAS  Google Scholar 

  • Ramwani J, Moscarello MA (1990): Phosphorylation of charge isomers (components) of human myelin basic protein: identification of phosphorylated sites. J Neurochem 55: 1703–1710.

    Article  PubMed  CAS  Google Scholar 

  • Readhead C, Popko B, Takahashi H, Shine HD, Saavedra RA, Sidman RL, Hood L (1987): Expression of myelin basic protein gene in transgenic shiverer mice: correction of the dysmyelinating phenotype. Cell 48: 703–712.

    Article  PubMed  CAS  Google Scholar 

  • Roth HS, Kronquist KE, Kelero de Rosbo N, Crandall BF, Campagnoni AT (1987): Evidence for the expression of four myelin basic protein variants in the developing human spinal cord through cDNA cloning. J Neurosci Res 17: 321–328.

    Google Scholar 

  • Schwob VS, Clark HB, Agrawal D, Agrawal HC (1985): Electron microscopic immunocytochemical localization of myelin proteolipid protein and myelin basic protein to oligodendrocytes in rat brain during myelination. J Neurochem 45: 559–571.

    Article  PubMed  CAS  Google Scholar 

  • Simons-Johnson R, Roder JC, Riordan JR (1995): Overexpression of the DM20 myelin proteolipids causes central nervous system demyelination in transgenic mice. J Neurochem 64: 967–976.

    Article  Google Scholar 

  • Simpson L, Emeson RB (1996): RNA editing. Annu Rev Neurosci 19: 27–52.

    Article  PubMed  CAS  Google Scholar 

  • Small DH, Carnegie PR (1982): In vivo methylation of an arginine in chicken myelin basic protein. J Neurochem 38: 184–190.

    Article  PubMed  CAS  Google Scholar 

  • Staugaitis SM, Colman DR, Pedraza L (1996): Membrane adhesion and other functions of the myelin basic proteins. Bioessays 18: 13–18.

    Article  PubMed  CAS  Google Scholar 

  • Stemberger NH, Iyotoma Y, Kies MW, Webster H de F (1978): Immunocytochemical method to identify basic protein in myelin forming oligodendrocytes of newborn rat CNS. J Neurocytol 7: 251–263.

    Article  Google Scholar 

  • Stewart R, Boggs JM (1993): A carbohydrate-carbohydrate interaction between galactosylceramide-containing liposomes and cerebroside sulfate-containing liposomes: dependence on the glycolipid ceramide composition. Biochem 32: 10666–10674.

    Article  CAS  Google Scholar 

  • Stoner GL (1984): Pedicted folding of 13-structure in myelin basic protein. J Neurochem 43: 433–447.

    Article  PubMed  CAS  Google Scholar 

  • Streicher R, Stoffel W (1989): The organization of the human myelin basic protein gene: comparison with the mouse. Biol Chem Hoppe Seyler 370: 503–510.

    Article  PubMed  CAS  Google Scholar 

  • Tompkins TA, Moscarello MA (1991): A 57 kDa phosphatidylinositol-specific phospholipase C from bovine brain. J Biol Chem 266: 4228–4236.

    PubMed  CAS  Google Scholar 

  • Tompkins TA, Moscarello MA (1993): Stimulation of bovine brain phospholipase C activity by myelin basic protein requires arginyl residues in peptide linkage. Arch Biochem Biophys 302: 476–483.

    Article  PubMed  CAS  Google Scholar 

  • Tsai SC, Noda M, Adamik R, Chang PP, Chen HC, Moss J, Vaughan M (1988): Stimulation of choleragen enzymatic activities by GTP and two soluble proteins purified from bovine brain. J Biol Chem 263: 1768–1772.

    PubMed  CAS  Google Scholar 

  • Turner RS, Chou C-H J, Kibler RF, Kuo JF (1982): Basic protein in brain myelin is phosphorylated by endogenous phospholipid sensitive calcium-dependent protein kinase. J Neurochem 39: 1397–1404.

    Article  PubMed  CAS  Google Scholar 

  • Ulimer JB (1988): The phosphorylation of myelin proteins. Prog Neurobiol 31: 241–259.

    Article  Google Scholar 

  • Ursell MRM, McLaurin J, Wood DD, Ackerley CA, Moscarello MA (1995): Localization and partial characterization of a 60 kDa citrulline-containing transport form of myelin basic protein from MO3–13 cells and human white matter. J Neurosci Res 42: 41–53.

    Article  PubMed  CAS  Google Scholar 

  • Van Dop C, Tsubokawa M, Bourne HR, Ramachandran J (1984): Amino acid sequence of retinal transducin at the site ADP-ribosylated by cholera toxin. J Biol Chem 259: 696–696.

    PubMed  Google Scholar 

  • Vandekerckhove J, Schering B, Barmann M, Aktories K (1987): Clostridium perfringens iota toxin ADP-ribosylates skeletal muscle actin in Arg 177. Febs Letts 225: 48–52.

    Article  CAS  Google Scholar 

  • Wood DD, Moscarello MA (1989): The isolation, characterization and lipid aggregating properties of a citrullinecontaining myelin basic protein. J Biol Chem 264: 5121–5127.

    PubMed  CAS  Google Scholar 

  • You SM, Ackerley CA, Mastronardi FG, Groome N, Moscarello MA (1996): Identification of a mitogen-activated protein kinase site in human myelin basic protein in situ. J Neuroimmunol 65: 55–59.

    Article  Google Scholar 

  • Yoon 1-S, Moscarello MA (1996): ADP-ribosyltransferase activity of myelin. M.Sc. Thesis, University of Toronto, Toronto, Canada.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biochemistry Research, Hospital for Sick Children, Toronto, Ontario, M5G 1X8, Canada

    Mario A. Moscarello

Authors
  1. Mario A. Moscarello
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. University of Saskatchewan, Saskatoon, Saskatchewan, Canada

    Bernhard H. J. Juurlink , Richard M. Devon , J. Ronald Doucette , Adil J. Nazarali , David J. Schreyer  & Valerie M. K. Verge , , , ,  & 

Rights and permissions

Reprints and permissions

Copyright information

© 1997 Springer Science+Business Media New York

About this chapter

Cite this chapter

Moscarello, M.A. (1997). Myelin Basic Protein, the “Executive” Molecule of the Myelin Membrane. In: Juurlink, B.H.J., Devon, R.M., Doucette, J.R., Nazarali, A.J., Schreyer, D.J., Verge, V.M.K. (eds) Cell Biology and Pathology of Myelin. Altschul Symposia Series, vol 4. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5949-8_2

Download citation

  • DOI: https://doi.org/10.1007/978-1-4615-5949-8_2

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-0-306-45595-7

  • Online ISBN: 978-1-4615-5949-8

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation