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Mapping the Redox State of CHOP-Treated Non-Hodgkin’s Lymphoma Xenografts in Mice

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Oxygen Transport to Tissue XXXV

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 789))

Abstract

Drug treatment may alter the metabolism of cancer cells and may alter the mitochondrial redox state. Using the redox scanner that collects the fluorescence signals from both the oxidized flavoproteins (Fp) and the reduced form of nicotinamide adenine dinucleotide (NADH) in snap-frozen tumor tissues, we investigated the effects of chemotherapy on mouse xenografts of a human diffuse large B-cell lymphoma cell line (DLCL2). The mice in the treatment group were treated with CHOP – cyclophosphamide (C) + hydroxydoxorubicin (H) + Oncovin (O) + prednisone (P) using the following regimen: CHO administration on day 1 followed by prednisone administration on day 1–5. On day 5 the mitochondrial redox state of the treated group was slightly more reduced than that of the control group (p = 0.049), and the Fp content of the treated group was significantly decreased (p = 0.033).

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Acknowledgments

Many thanks to Dr. Huaqing Zhao for valuable discussions about statistical analysis. This work was supported by the Center of Magnetic Resonance and Optical Imaging (CMROI) – an NIH-supported research resource P41RR02305 (R. Reddy), the Small Animal Imaging Resources Program (SAIR) 2U24-CA083105 (J.D. Glickson & L. Chodosh), 2R01-CA101700 (J.D. Glickson), and NIH k99/R00-CA126187 (R. Choe).

Author information

Authors and Affiliations

  1. Department of Radiology, University of Pennsylvania, Philadelphia, PA, USA

    He. N. Xu, Tahreem A. Mir, Seung-Cheol Lee, Min Feng, Namisa Farhad, Jerry D. Glickson & Lin Z. Li

  2. Britton Chance Laboratory of Redox Imaging, Johnson Research Foundation, Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, PA, USA

    He. N. Xu, Tahreem A. Mir, Min Feng, Namisa Farhad & Lin Z. Li

  3. Department of Biomedical Engineering, University of Rochester, Rochester, NY, USA

    Regine Choe

  4. Abramson Cancer Center, University of Pennsylvania, Philadelphia, PA, USA

    Lin Z. Li

  5. Institute of Translational Medicine and Therapeutics, University of Pennsylvania, Philadelphia, PA, USA

    Lin Z. Li

Authors
  1. He. N. Xu
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  2. Tahreem A. Mir
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  3. Seung-Cheol Lee
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  4. Min Feng
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  5. Namisa Farhad
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  6. Regine Choe
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  7. Jerry D. Glickson
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  8. Lin Z. Li
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Corresponding author

Correspondence to Lin Z. Li .

Editor information

Editors and Affiliations

  1. Department of Electrical Engineering and iMinds Future Health Department, KU Leuven, Leuven, Belgium

    Sabine Van Huffel  & Alexander Caicedo  & 

  2. Neonatal Intensive Care Unit KU Leuven, University Hospitals, Leuven, Belgium

    Gunnar Naulaers

  3. Synthesizer, Inc., Ellicott City, MD, USA

    Duane F. Bruley

  4. Microvascular Measurements, Lorenzen, Italy

    David K. Harrison

Additional information

This article is dedicated to the memory of late Dr. Britton Chance who participated in the study with extraordinary scientific enthusiasm at the age of 97.

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© 2013 Springer Science+Business Media New York

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Xu, H.N. et al. (2013). Mapping the Redox State of CHOP-Treated Non-Hodgkin’s Lymphoma Xenografts in Mice. In: Van Huffel, S., Naulaers, G., Caicedo, A., Bruley, D.F., Harrison, D.K. (eds) Oxygen Transport to Tissue XXXV. Advances in Experimental Medicine and Biology, vol 789. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-7411-1_33

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