Abstract
Thiopental, a well-known barbiturate, is often used in patients who are at high risk of developing cerebral ischemia, especially during brain surgery. Although barbiturates are known to affect a variety of processes in the cerebral cortex, including oxygen consumption by the mitochondria, the interrelation between mitochondrial function and anesthetics has not been investigated in detail under in vivo conditions.
The aim of this study was to examine the effects of thiopental on brain functions in normoxia and under partial or complete ischemia. The use of the multiparametric monitoring system permitted simultaneous measurements of microcirculatory blood flow, NADH fluorescence, tissue reflectance, and ionic and electrical activities of the cerebral cortex. Thiopental caused a significant, dose-dependent decrease in blood flow and a significant decrease in extracellular levels of potassium, with no significant changes in NADH levels in normoxic and ischemic rats. Following complete ischemia (death), the increase in the reflectance was significantly smaller in the anesthetized normoxic group versus the awake normoxic group. The time until the secondary increase in reflectance, seen in death, was significantly shorter in the anesthetized ischemic group.
In conclusion, it seems that the protective effect of thiopental occurs only under partial ischemia and not under complete ischemia.
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Abstract
Z. Karwacki, P. Kowianski, and J. Morys, General anaesthesia in rats undergoing experiments on the central nervous system, Folia Morphol. (Warsz. ) 60, 235-242 (2001)
S. Muravchick and R. J. Levy, Clinical implications of mitochondrial dysfunction, Anesthesiology. 105, 819- 837 (2006)
K. Nouette-Gaulain, A. Quinart, T. Letellier, and F. Sztark, Mitochondria in anaesthesia and intensive care, Ann. Fr. Anesth. Reanim. 26, 319-333 (2007)
J. C. Drummond and H. M. Shapiro, Cerebral physiology, in: Anesthesia. edited by. R.D. Miller (Churchill Livingstone, New York, 1990), pp. 621-658.
B. K. Siesjo, Anaesthesia, analgesia and sedation. in: Brain Energy Metabolism.Edited by B.K. Siesjo. (John Wiley & Sons, New York, 1978), pp. 233-255.
P. Hans and V. Bonhomme, The rationale for perioperative brain protection, Eur. J. Anaesthesiol. 21, 1-5 (2004)
R. Hemmingsen, D. I. Barry, and M. M. Hertz, Cerebrovascular effects of central depressants: a study of nitrous oxide, halothane, pentobarbital and ethanol during normocapnia and hypercapnia in the rat, Acta Pharmacol. Toxicol. (Copenh) 45, 287-295 (1979)
A. Mayevsky, R. Nakache, H. Merhav, M. Luger-Hamer, and J. Sonn, Real time monitoring of intraoperative allograft vitality, Transplant. Proc. 32, 684-685 (2000)
A. Mayevsky and G. Rogatsky, Mitochondrial function in vivoevaluated by NADH fluorescence: From animal models to human studies, Am. J. Physiol Cell Physiol. 292, C615-C640 (2007)
D. Arvidsson, H. Svensson, and U. Haglund, Laser-Doppler flowmetry for estimating liver blood flow, Am J Physiol 254, G471-G476 (1988)
K.C. Wadhwani and S. I. Rapoport, Blood flow in the central and peripheral nervous systems. in: Laser Doppler Blood Flowmetry,edited by A.P. Shepherd and P.A Oberg, (Kluwer Academic Pub., Boston, 1990), pp. 265-304.
C. M. Friedli, D. S. Sclarsky, and A. Mayevsky, Multiprobe monitoring of ionic, metabolic and electrical activities in the awake brain, Am. J. Physiol. 243, R462-R469 (1982)
J. Donegan, Effect of anesthesia on cerebral physiology and metabolism. in: Neuroanesthesia: Handbook of Clinical and Physiologic Essentials. Edited by P. Newfield, J.E Cottrell and C.B Wilson, (Little Brown and Company, New York, 1991), pp. 17-29.
E. M. Nemoto, R. Klementavicius, J. A. Melick, and H. Yonas, Suppression of cerebral metabolic rate for oxygen (CMRO2) by mild hypothermia compared with thiopental, J Neurosurg Anesthesiol. 8, 52-59 (1996)
P. Arhem and H. Kristbjarnarson, A barbiturate-induced potassium permeability increase in the myelinated nerve membrane, Acta Physiol Scand. 113, 387-392 (1981)
R. Z. Kozlowski, C. N. Hales, and M. L. Ashford, Dual effects of diazoxide on ATP-K+ currents recorded from an insulin-secreting cell line, Br. J Pharmacol. 97, 1039-1050 (1989)
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Dekel, N., Sonn, J., Barbiro-Michaely, E., Ornstein, E., Mayevsky, A. (2009). Effects of Anesthesia on Brain Mitochondrial Function, Blood Flow, Ionic And Electrical Activity Monitored in Vivo. In: Liss, P., Hansell, P., Bruley, D.F., Harrison, D.K. (eds) Oxygen Transport to Tissue XXX. Advances in Experimental Medicine and Biology, vol 645. Springer, Boston, MA. https://doi.org/10.1007/978-0-387-85998-9_8
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DOI: https://doi.org/10.1007/978-0-387-85998-9_8
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