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Epidermal Cells

Methods and Protocols

  • Book
  • © 2005

Overview

Editors:
  1. Kursad Turksen

    1. Ottawa Health Research Institute, Ottawa, Canada

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Part of the book series: Methods in Molecular Biology (MIMB, volume 289)

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Table of contents (42 protocols)

  1. Front Matter

    Pages i-xv
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  2. Keratinocyte and Organ Cultures

    1. Front Matter

      Pages 1-1
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    2. Primary Mouse Keratinocyte Culture

      • Annalisa Pirrone, Barbara Hager, Philip Fleckman
      Pages 3-14

    3. Serial Cultivation of Primary Adult Murine Keratinocytes

      • Richard P. Redvers, Pritinder Kaur
      Pages 15-22

    4. Keratinocyte Culture in the Absence of Substrate Attachment

      • Monika Jost, Ulrich Rodeck
      Pages 23-28

    5. Application of Genetically Modified Feeder Cells for Culture of Keratinocytes

      • Takashi Kameda, Toshihiro Sugiyama
      Pages 29-38

    6. Organ Culture of Developing Mouse Skin and Its Application for Molecular Mechanistic Studies of Morphogenesis

      • Mariko Kashiwagi, Nam-ho Huh
      Pages 39-45

    7. Experimental Models to Analyze Differentiation Functions of Cultured Keratinocytes In Vitro and In Vivo

      • Nicole Maas-Szabowski, Norbert E. Fusenig, Hans-Jürgen Stark
      Pages 47-60

    8. In Vitro Fabrication of Engineered Human Skin

      • Alexander Margulis, Weitian Zhang, Jonathan A. Garlick
      Pages 61-70

  3. Epidermal Stem Cells

    1. Front Matter

      Pages 71-71
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    2. In Vivo Labeling and Analysis of Epidermal Stem Cells

      • Wei-Yang Wu, Rebecca J. Morris
      Pages 73-77

    3. Method for the Harvest and Assay of In Vitro Clonogenic Keratinocytes Stem Cells From Mice

      • Wei-Yang Wu, Rebecca J. Morris
      Pages 79-86

    4. FACS Enrichment of Human Keratinocyte Stem Cells

      • Amy Li, Pritinder Kaur
      Pages 87-96

    5. Isolation, Characterization, and Culture of Epithelial Stem Cells

      • Jackie R. Bickenbach
      Pages 97-102

    6. Keratin 19 as a Stem Cell Marker In Vivo and In Vitro

      • Danielle Larouche, Cindy Hayward, Kristine Cuffley, Lucie Germain
      Pages 103-110

  4. Analysis of Epidermal Differentiation

    1. Front Matter

      Pages 111-111
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    2. Immunolocalization in the Epidermis

      • Tammy-Claire Troy, Ramtin Rahbar, Bilge Diker, Kursad Turksen
      Pages 113-119

    3. Epidermal Cell Analysis by RT-PCR

      • Tammy-Claire Troy, Robert Man-Kit Cheung, Kursad Turksen
      Pages 121-126

    4. Whole-Mount Assays for Gene Induction and Barrier Formation in the Developing Epidermis

      • Carolyn Byrne, Matthew J. Hardman
      Pages 127-136

    5. Analysis of Early Epidermal Development in Zebrafish

      • Ashley E. Webb, David Kimelman
      Pages 137-145
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Keywords

About this book

A collection of cutting-edge methods to analyze and manipulate epidermal cellprecursors and mature epidermal cells. These protocols cover different methods and models for culturing epidermal cells, for enriching very early epidermal progenitors, and for studying epidermal cell commitment and differentiation both in vitro and in vivo. Topics of special interest include the derivation, characterization, and utility of epidermal stem cells, mature epidermal cells and their characterization, and applications in regenerative medicine. These readily reproducible techniques broaden our understanding of the biology of epidermal cells and of their utility in normal tissue homeostasis and regenerative medicine applications.

Reviews

"The protocols are well written and fully detailed; they combine basic science protocols with informative diagrams illustrating results...will become an invaluable reference guide for laboratory researchers interested in mammalian epidermal cells." Doody's Health Sciences Book Review Journal

"For anyone who is planning or is already practically involved in experimental dermatological research, this impressive book is a must in the laboratory." -Acta Dermato-Venereologica

 

Editors and Affiliations

  • Ottawa Health Research Institute, Ottawa, Canada

    Kursad Turksen

Bibliographic Information

  • Book Title: Epidermal Cells

  • Book Subtitle: Methods and Protocols

  • Editors: Kursad Turksen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592598307

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2005

  • eBook ISBN: 978-1-59259-830-4Published: 03 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: 480

  • Number of Illustrations: 100 b/w illustrations, 8 illustrations in colour

  • Topics: Internal Medicine, Cell Biology

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