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Polyamine Protocols

  • Book
  • © 1998

Overview

Editors:
  1. David M. L. Morgan

    1. King’s College, London, UK

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Part of the book series: Methods in Molecular Biology (MIMB, volume 79)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-ix
    Download chapter PDF

  2. Introduction

    1. Polyamines: An Introduction

      • David M. L. Morgan
      Pages 3-30

  3. Assay Methods for Enzymes of Polyamine Biosynthesis

    1. Determination of Ornithine Decarboxylase Activity Using [3H]Omithine

      • Amalia Tabib
      Pages 33-39

    2. Assay of Mammalian Ornithine Decarboxylase Activity Using [14C]Ornithine

      • Catherine S. Coleman, Anthony E. Pegg
      Pages 41-44

    3. Assay of Mammalian S-Adenosylmethionine Decarboxylase Activity

      • Lisa M. Shantz, Anthony E. Pegg
      Pages 45-50

    4. Assay of Spermidine and Spermine Synthases

      • Laurie Wiest, Anthony E. Pegg
      Pages 51-57

    5. Measurement of Spermidine/Spermine N 1-Acetyltransferase Activity

      • Heather M. Wallace, Deborah M. Evans
      Pages 59-68

    6. Assay of Spermidine N 8-Acetyltransferase

      • Jim Blankenship
      Pages 69-73

  4. Assay Methods for Enzymes of Polyamine Catabolism

    1. Assay of N 8-Acetylspermidine Deacetylase

      • Jim Blankenship
      Pages 77-80

    2. Hydrogen Peroxide Assay for Amine Oxidase Activity

      • R. James Storer, Antonio Ferrante
      Pages 81-90

    3. Radiochemical Assay of Diamine Oxidase

      • R. James Storer, Antonio Ferrante
      Pages 91-95

    4. Radiochemical Estimation of Polyamine Oxidase

      • David M. L. Morgan
      Pages 97-103

    5. Measurement of Aldehyde Formation as an Assay for Polyamine Oxidase Activity

      • David M. L. Morgan
      Pages 105-108

  6. Measurement of Polyamine Transport

    1. Measurement of Polyamine Transport: Adherent Cells

      • David M. L. Morgan
      Pages 139-147

    2. Measurement of Polyamine Transport: Cells in Suspension

      • Sarah A. Le Quesne, Alan H. Fairlamb
      Pages 149-156

    3. Measurement of Polyamine Efflux from Cells in Culture

      • Heather M. Wallace, A. Jill Mackarel
      Pages 157-165
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About this book

David Morgan brings together in Polyamine Protocols a unique collection of hands-on enzyme assay techniques for the study of polyamines and their biological functions. The techniques range from assay methods for enzymes of polyamine biosynthesis and catabolism to measurements of polyamines, polyamine transport, and polyamine effects on cell growth. All methods are presented by active researchers who have developed them to a high degree of perfection in their own laboratories, and include clear, step-by-step instructions with numerous hints and tips to ensure readily reproducible results.

Polyamine Protocols is the only compilation of methods currently available for use in studies involving polyamines. The collection offers both novice and experienced researchers across many fields-including cell and molecular biology, pharmacology, oncology, physiology, laboratory medicine, and clinical pathology-all the tools needed to elucidate the biological roles played by these ubiquitous substances.

Reviews

"...This book would be a useful reference for groups already working in this area and an excellent starting point for those new to the subject."-Molecular Biotechnology

"The collection of gene therapy protocols is a valuable and indispensable resource for basic scientists, graduate students, and postdoctoral fellows. The book represents an outstanding work, which should not be missed in all laboratories dealing with gene therapy." - Neoplasma

Editors and Affiliations

  • King’s College, London, UK

    David M. L. Morgan

Bibliographic Information

  • Book Title: Polyamine Protocols

  • Editors: David M. L. Morgan

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/0896034488

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 1998

  • Hardcover ISBN: 978-0-89603-448-8Published: 10 October 1997

  • Softcover ISBN: 978-1-61737-042-7Published: 10 November 2010

  • eBook ISBN: 978-1-59259-565-5Published: 02 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: IX, 186

  • Topics: Biochemistry, general

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