Abstract
MJM1968, an actinomycete showing potent antifungal activity, was isolated and identified by 16S rDNA sequence analysis as Streptomyces malaysiensis. MJM1968 showed strong antifungal activity on phytopathogenic fungi such as Fusarium oxysporum, Rhizoctonia solani, Cladosporium cladosporioides, Fusarium chlamydosporum, Colletotrichum gloeosporioides KACC 40693, Alternaria mali KACC 40026, and Pestalotia spp. KACC 40501, in vitro. An antifungal compound was isolated from culture filtrate of strain MJM1968 by a series of chromatographic methods. Treatment of agricultural soil with strain MJM1968 mycelia reduced the native fungal population by more than 60% after 14 days. Treatment of soil with 10 mg/mL partially purified extract lowered native fungal density by more than 80% after 14 days. The compound, which exhibited antifungal activity in a broad range of pH and temperature, was identified as azalomycin F complex by 1H and 13C NMRs. This is the first report on the isolation of azalomycin F complex from S. Malaysiensis, demonstrating a broad-spectrum suppression of fungal pathogens in agricultural soil.
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References
Bowers JH and Locke JC (2000) Effect of botanical extracts on the population density of Fusarium oxysporum in soil and control of Fusarium wilt in the greenhouse. Plant Dis 84, 300–305.
Bowers JH and Locke JC (2004) Effect of formulated plant extracts and oils on population density of Phytophthora nicotianae in soil and control of Phytophthora blight in the greenhouse. Plant Dis 88, 11–16.
Chandra A and Nair MG (1995) Azalomycin F complex from Streptomyces hygroscopicus, MSU/MN-4-75B. J Antibiot 48, 896–898.
Chung WC, Huang JW, and Huang HC (2005) Formulation of a soil biofungicide for control of damping-off of Chinese cabbage (Brassica chinensis) caused by Rhizoctonia solani. Biol Control 32, 287–294.
Coombs JT and Franco CMM (2003) Isolation and Identification of Actinobacteria from Surface-Sterilized Wheat Roots. Appl Environ Microbiol 69, 5603–5608.
Fajola AO (1979) The post-harvest fruit rots of tomato (Lycopersicum esculentum) in Nigeria. Nahrung 23, 105–109.
Hwang BK and Kim BS (1995) In vivo efficacy and in vitro activity of tubercidin, an antibiotic nucleoside, for control of Phytophthora capsici blight in Capsicum annum. Pestic Sci 44, 255–260.
Hwang BK, Lim SW, Kim BS, Lee JY, and Moon SS (2001) Isolation and In vivo antifungal activity of phenylacetic acid and sodium phenylacetate from Streptomyces humidus. Appl Environ Microbiol 67, 3739–3645.
Isono K, Nagatsu J, Kobinata K, Sasaki K, and Suzuki S (1965) Studies on Polyoxins, antifungal antibiotics. Part I. Isolation and characterization of polyoxin A and B. Agric Biol Chem 29, 848–854.
Iwasa T, Higashide E, Yamamoto H, and Shibata M (1970) Studies on validamycins, new antibiotics II. Production and biological properties of validamycin A and B. J Antibiot 23, 595–602.
Leben C and Keitt GW (1954) Antibiotics and plant disease: Effects of antibiotics in control of plant diseases. J Agric Food Chem 2, 234–239.
Lucas JA, Bower LA, and Coffey MD (1990) Fungicide resistance in soilborne Phytophthora species. EPPO Bulletin 20, 199–206.
Martin FN and Bull CT (2002) Biological approaches for control of root pathogens of strawberry. Phytopathology 92, 1356–1362.
Martin HL (2003) Management of soilborne diseases of beet root in Australia: a review. Aust J Exp Agr 43, 1281–1292.
Minuto A, Spadaro D, Garibaldi A, and Gullino ML (2006) Control of soilborne pathogens of tomato using a commercial formulation of Streptomyces griseoviridis and solarization. Crop Prot 25, 468–475.
Morales-Rodríguez I, Yañez-Morales MJ, Silva-Rojas HV, García-de-Los-Santos G, and Guzmán-de-Peña DA (2007) Biodiversity of Fusarium species in Mexico associated with ear rot in maize, and their identification using a phylogenetic approach. Mycopathologia 163, 31–39.
Nair MG, Chandra A, and Thorogood DL (1994) Gopalamicin, an antifungal macrolide produced by soil actinomycete. J Agric Food Chem 42, 2308–2310.
Raaijmakers JM, Paulitz TC, Steinberg C, Alabouvette C, and Moenne-Loccoz Y (2009) The rhizosphere: a playground and battlefield for soilborne pathogens and beneficial microorganisms. Plant Soil 321, 341–361.
Saitou N and Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
Shen YC (1996) Twenty-five years of research on Jinggangmycin and exploitation of this antibiotic as a biofungicide. Plant Prot 22, 44–45.
Shimura M, Watanabe T, Ohashi T, Shomura T, Niida T, Sekizawa Y, and Umezawa H (1970) Efficacy of bleomycin in the protection of rice sheath blight disease. J Antibiot 3, 166–167.
Stepień L, Popiel D, Koczyk G, and Chełkowski J (2008) Wheat-infecting Fusarium species in Poland-their chemotypes and frequencies revealed by PCR assay. J Appl Genet 49, 433–441.
Takeuchi S, Hirayama K, Ueda K, Sakai H, and Yonehara H (1957) Blasticidin S, a new antibiotic. J Antibiot 11, 1–5.
Tamura K, Dudley J, Nei M, and Kumar S (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 24, 1596–1599.
Termorshuizen AJ and Jeger MJ (2008) Strategies of soilborne plant pathogenic fungi in relation to disease suppression. Fungal Ecol 108–114.
Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, and Higgins DG (1997) CLUSTALX, ver. 1.81: the CLUSTALX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 24, 4876–4882.
Umezawa H, Okami Y, Hashimoto T, Suhara Y, Hamada M, and Takeuchi T (1965) A new antibiotic kasugamycin. J Antibiot 18, 101–103.
Watve MG, Tickoo R, Jog MM, and Bhole BD (2001) How many antibiotics are produced by the genus Streptomyces? Arch Microbiol 176, 386–390.
White TJ, Bruns T, Lee S, and Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR Protocols: a Guide to Methods and Applications, Innis MA, Gelfand DH, Sninsky JJ, and White TJ (eds.), pp. 315–322. Academic Press, New York, NY, USA.
Wicklow DT and Poling SM (2009) Antimicrobial activity of pyrrocidines from Acremonium zeae against endophytes and pathogens of maize. Phytopathology 99, 109–15.
Yuan WM and Crawford DL (1995) Characterization of Streptomyces lydicus WYEC108 as a potential biocontrol agent against fungal root and seed rots. Appl Environ Microbiol 61, 3119–3128.
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Cheng, J., Yang, S.H., Palaniyandi, S.A. et al. Azalomycin F complex is an antifungal substance produced by Streptomyces malaysiensis MJM1968 isolated from agricultural soil. J. Korean Soc. Appl. Biol. Chem. 53, 545–552 (2010). https://doi.org/10.3839/jksabc.2010.084
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DOI: https://doi.org/10.3839/jksabc.2010.084