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Increasing the resistance of rape plants to the parasitic nematode Heterodera schachtii using RNAi technology

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Abstract

A vector for the constitutive expression of antisense to the conservative region of the 8H07 gene of the nematode H.schachtii dsRNA is constructed and a genetic transformation of rape plants is conducted by means of A. tumefaciens. Using molecular genetic methods, the presence of the vector expression of antinematode dsRNA in the genome of transgenic rape plants is shown, as well as the high level of their silencing activity is confirmed both in nematodes and in infected plants. In laboratory studies, a considerable increase in the tolerance of transgenic rape plants to the root parasitic nematode H. schachtii was shown for physiological signs.

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Authors and Affiliations

  1. Institute of Bioorganic Chemistry and Petrochemistry, National Academy of Sciences of Ukraine, Kyiv, Ukraine

    V. A. Tsygankova

  2. Institute of Food Biotechnology and Genomics, National Academy of Sciences of Ukraine, Kyiv, Ukraine

    A. I. Yemets, A. P. Galkin & Ya. B. Blume

  3. Zabolotny Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kyiv, Ukraine

    H. O. Iutinska & L. O. Beljavska

Authors
  1. V. A. Tsygankova
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  2. A. I. Yemets
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  3. H. O. Iutinska
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  4. L. O. Beljavska
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  5. A. P. Galkin
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  6. Ya. B. Blume
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Correspondence to V. A. Tsygankova.

Additional information

Original Ukrainian Text © V.A. Tsygankova, A.I. Yemets, H.O. Iutinska, L.O. Beljavska, A.P. Galkin, Ya.B. Blume, 2013, published in Tsitologiya i Genetika, 2013, Vol. 47, No. 4, pp. 35–45.

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Tsygankova, V.A., Yemets, A.I., Iutinska, H.O. et al. Increasing the resistance of rape plants to the parasitic nematode Heterodera schachtii using RNAi technology. Cytol. Genet. 47, 222–230 (2013). https://doi.org/10.3103/S0095452713040105

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  • DOI: https://doi.org/10.3103/S0095452713040105

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