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Isolation and amplification of cDNA from the conserved region of the nematode Heterodera schachtii 8H07 gene with a close similarity to its homolog in rape plants

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Abstract

An original method was developed for isolation of small regulatory RNAs (si/miRNAs) from plant cells. PCR-amplification was carried out for the cDNA fragment of the nematode Heterodera schachtii 8H07 gene. Northern-blot hybridization of plant si/miRNAs with the cDNA fragment of the conserved region from the nematode’s 8H07 gene confirmed the high degree of their homology. In the future, the amplified cDNA fragment of the nematode 8H07 gene will be used for creating a recombinant gene with an antisense dsRNA sequence for increasing the resistance of rape plants to parasitic nematodes.

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Authors and Affiliations

  1. Institute of Bioorganic Chemistry and Petrochemistry, National Academy of Sciences of Ukraine, Kyiv, Ukraine

    V. A. Tsygankova & Ya. V. Andrusevich

  2. Interdepartmental Scientific and Technological Center “Agrobiotech”, National Academy of Sciences of Ukraine and Ministry of Education and Sciences of Ukraine, Kyiv, Ukraine

    S. P. Ponomarenko

  3. Institute of Food Biotechnology and Genomics, National Academy of Sciences of Ukraine, Kyiv, Ukraine

    A. P. Galkin & Ya. B. Blume

Authors
  1. V. A. Tsygankova
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  2. Ya. V. Andrusevich
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  3. S. P. Ponomarenko
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  4. A. P. Galkin
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  5. Ya. B. Blume
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Correspondence to V. A. Tsygankova.

Additional information

Original Ukrainian Text © V.A. Tsygankova, Ya.V. Andrusevich, S.P. Ponomarenko, A.P. Galkin, Ya.B. Blume, 2012, published in Tsitologiya i Genetika, 2012, Vol. 46, No. 6, pp. 3–11.

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Tsygankova, V.A., Andrusevich, Y.V., Ponomarenko, S.P. et al. Isolation and amplification of cDNA from the conserved region of the nematode Heterodera schachtii 8H07 gene with a close similarity to its homolog in rape plants. Cytol. Genet. 46, 335–341 (2012). https://doi.org/10.3103/S0095452712060114

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  • DOI: https://doi.org/10.3103/S0095452712060114

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