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Formation of multicellular aggregates under different conditions of microenvironment

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Abstract

Multicellular aggregates (spheroids) represent an intermittent level between monolayer growing cells and tissue culture. Spheroids are rather objective model of the three-dimensional growth and organization, the cell-to-cell interactions and influence of microenvironmental conditions on tumour microaggre-gates. In our work formation and growth of spheroids depends on concentration of CMC and FCS. Conditions of microenvironment influence on intensiveness of proliferation as well as on cells adhesiveness and formation of microaggregates.

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Authors and Affiliations

  1. Department of Biotecftntcal Problems of Diagnostics Institute Problems of Cryobiology and Criomedicine NAN Ukraine, Kiev, Ukraine

    L. V. Garmanchuk, E. M. Perepelitsyna & M. V. Sydorenko

  2. Taras Shevchenko Kiev National University, Kiev, Ukraine

    L. I. Ostapchenko

Authors
  1. L. V. Garmanchuk
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  2. E. M. Perepelitsyna
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  3. M. V. Sydorenko
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  4. L. I. Ostapchenko
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Correspondence to L. V. Garmanchuk.

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Garmanchuk, L.V., Perepelitsyna, E.M., Sydorenko, M.V. et al. Formation of multicellular aggregates under different conditions of microenvironment. Cytol. Genet. 44, 19–22 (2010). https://doi.org/10.3103/S0095452710010044

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  • DOI: https://doi.org/10.3103/S0095452710010044

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