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Effect of two plant extracts and Lactobacillus fermentum on colonization of gastrointestinal tract by Salmonella enterica var. Düsseldorf in chicks

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Abstract

According to published data, it is well known that essential oils from plants possess antimicrobial activity against a wide range of pathogens, including Salmonella strains. The influence of the administration of essential oils from oregano (Origanum vulgare) and thyme (Thymus vulgaris) as well as Lactobacillus fermentum on crop, caecum, liver and spleen colonization by Salmonella enterica var. Düsseldorf in chicks was investigated in this study. For the experiment, one hundred 1-day old chicks were used, and they were divided into four groups: (i) untreated (C); (ii) treated with L. fermentum (L); (iii) treated with a mixture of oregano and thyme essential oils (OT); and (iv) treated with a combination of L. fermentum and a mixture of oregano and thyme essential oils (LOT). Essential oils from oregano and thyme were mixed with commercial poultry diet, which was offered ad libitum to chicks in appertaining groups. L. fermentum was added daily to drinking water. After 3 days all the chicks were challenged orally with S. enterica var. Düsseldorf. The crops, ceca, spleens and livers of the birds were examined for S. enterica var. Düsseldorf colonization 5 days after the challenge. Our results showed that a combined administration of L. fermentum and essential oils (oregano and thyme) in group with combined application of essential oils and lactobacillus strain reduced the percentage of colonized crops and ceca when compared to the control group without any treatment.

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Authors and Affiliations

  1. Institute of Gnotobiology and Prevention of Diseases in Young, University of Veterinary Medicine, Komenského 73, SK-04181, Košice, Slovakia

    Jana Koščová, Radomíra Nemcová, Soňa Gancarčíková, Zuzana Jonecová, L’uboslava Sciranková & Viktor Buleca

  2. Institute of Experimental Medicine, Faculty of Medicine, Pavol Jozef Šafárik University, Trieda SNP 1, SK-04011, Košice, Slovakia

    Alojz Bomba

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  1. Jana Koščová
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  2. Radomíra Nemcová
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  3. Soňa Gancarčíková
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  4. Zuzana Jonecová
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  5. L’uboslava Sciranková
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  7. Viktor Buleca
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Correspondence to Jana Koščová.

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Koščová, J., Nemcová, R., Gancarčíková, S. et al. Effect of two plant extracts and Lactobacillus fermentum on colonization of gastrointestinal tract by Salmonella enterica var. Düsseldorf in chicks. Biologia 61, 775–778 (2006). https://doi.org/10.2478/s11756-006-0156-z

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  • DOI: https://doi.org/10.2478/s11756-006-0156-z

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