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Effect of salicin on induction and carbon catabolite repression of endoxylanase synthesis in Penicillium janthinellum MTCC 10889

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Abstract

Amongst various carbon sources, xylan was found to be the sole inducer of endoxylanase production by Penicillium janthinellum MTCC 10889 in submerged cultivation. Endoxylanase synthesis by a xylan induced culture was initially repressed after a simultaneous addition of xylose, probably by the inducer exclusion mechanism, but it was resumed and achieved its highest level at a much later stage of growth (at 120 h). Xylose added after 30 h of growth cannot exert its full repressive effect. Although glucose was proved to be a more potent repressor than xylose, supplementation of salicin, an alcoholic β-glycoside containing d-glucose, with pure xylan resulted in an about 3.22 fold increase in the enzyme synthesis at 72 h followed by constant high production of the enzyme at least until the 144th h of growth. Inducing capacity of salicin in a xylan induced culture was significantly reduced when it was added after 30 h of growth. Addition of salicin and xylan help to partially overcome the repressive effect of xylose and glucose. Failure of salicin in recovering the endoxylanase synthesis in actinomycin D and cyclohexamide inhibited the xylan induced culture indicating that salicin cannot initiate the de novo synthesis of the enzyme.

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  1. Microbiology Research Laboratory, Post Graduate Department of Zoology, Molecular Biology and Genetics, Presidency University, 86/1, College Street, Kolkata, 700 073, India

    Aditi Kundu & Rina Rani Ray

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  1. Aditi Kundu
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  2. Rina Rani Ray
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Correspondence to Rina Rani Ray.

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Kundu, A., Ray, R.R. Effect of salicin on induction and carbon catabolite repression of endoxylanase synthesis in Penicillium janthinellum MTCC 10889. Chem. Pap. 68, 451–456 (2014). https://doi.org/10.2478/s11696-013-0478-6

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  • DOI: https://doi.org/10.2478/s11696-013-0478-6

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