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Seroprevalence of Fasciola hepatica infection in cattle and sheep in the province of Kars, Turkey, as determined by ELISA

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Helminthologia

Summary

This study aimed to determine the seroprevalence of Fasciola hepatica infection in cattle and sheep in the province of Kars, Turkey. Serum samples from 500 cattle and 540 sheep, collected from 15 randomly selected localities (villages) in the region, were tested for the presence of anti-F. hepatica antibodies using an in-house ELISA test with 98 % sensitivity and 96 % specificity. The seroprevalence of F. hepatica in the district was determined to be 66.6 % (333/500) in cattle and 93 % (502/540) in sheep. There was also a statistically significant difference in the rates of seropositivity between villages (each of which could be considered to be a large herd or flock), ranging from 0 % to 100 % in cattle and from 68 % to 100 % in sheep, P < 0.01). These findings show that F. hepatica infection is very common in the region; that, in contrast to results from abattoir which indicate a level of only 10 % prevalence, the exposure of farm animals to the infection in the region is in fact very high; and that the risk of acquiring the infection varies between the localities and the host species tested within the region.

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References

  • Akca, A. (1999): Cytokine, cellular and humoral immune responses in calves experimentally-infected with Fasciola hepatica. PhD Thesis, UK, Liverpool: University of Liverpool

    Google Scholar 

  • Akyol, C. V. (2003): History and epidemiology. In: Tinar, R., Korkmaz, M. (Eds) Fasciolosis. İzmir, Turkey: Turkish Society for Parasitology, Publication no. 18: 85–106 (In Turkish)

    Google Scholar 

  • Duscher, R., Duscher, G., Hofer, J., Tichy, A., Prosl, H., Joachim, A. (2011): Fasciola hepatica - Monitoring the milky way? The use of tank milk for liver fluke monitoring in dairy herds as base for treatment strategies. Vet. Parasitol., 178, 273–278. DOI: 10.101/j.vetpar.2011.01.040

    Article  PubMed  Google Scholar 

  • Gargili, A., Tuzer, E., Gulanber, A., Toparlak, M., Efil, I., Keles, V., Ulutas, M. (1999): Prevalence of liver fluke infections in slaughtered animals in Trakya (Thrace) Turkey. Turk. J. Vet. Anim. Sci., 23, 115–116

    Google Scholar 

  • Gicik, Y., Arslan, M. O., Kara, M., Akca, A. (2002): Prevalence of liver flukes in sheep slaughtered in Kars province. Kafkas Univ. Vet. Fak. Derg., 8, 101–102 (In Turkish).

    Google Scholar 

  • Happich, F. A., Boray, J. C. (1969): Quantitative diagnosis of chronic fasciolosis. 1. Comparative studies on the quantitative faecal examinations for chronic Fasciola hepatica infection in sheep. Aust. Vet. J., 45,326 - 328

    Google Scholar 

  • Hillyer, G. V. (1999): Immunodiagnosis of human and animal fasciolosis. In: Dalton, J. P. (Ed) Fasciolosis. Wallingford, Oxon, UK: CABI Publishing, pp. 435–448

    Google Scholar 

  • Kurtpinar, H. (1957): Parasites of cattle, sheep and goats specific to the summer months in the provinces of Erzurum, Kars and Ağri, and the diseases they cause. Turk. Vet. Hek. Dern. Derg., 27, 3320–3325 (In Turkish)

    Google Scholar 

  • Leloglu, S. (1972): A comparative study of livers with and without liver Flukes (Fasciola hepatica) for clostridial infections by means of culture and by fluoroscent antibody technique. Turk. Vet. Hek. Dern. Derg., 42, 19–22

    Google Scholar 

  • Molloy, J. B., Anderson, G. R., Fletcher, T. I., Landmann, J., Knight, B. C. (2005): Evaluation of a commercially available enzyme linked immunosorbent assay for detecting antibodies to Fasciola hepatica and Fasciola gigantica in cattle, sheep and buffaloes in Australia. Vet. Parasitol., 130, 207–212. DOI: 10.1016/j.vetpar.2005.02.010

    Article  CAS  PubMed  Google Scholar 

  • Rapsch, C., Schweizer, G., Grimm, F., Kohler, L., Bauer, C., Deplazes, P., Braun, U., Torgerson, P. R. (2006): Estimating the true prevalence of Fasciola hepatica in cattle slaughtered in Switzerland in the absence of an absolute diagnostic test. Int. J. Parasitol., 36, 1153–1158. DOI: 10.1016/j.ijpara.2006.06.001

    Article  CAS  PubMed  Google Scholar 

  • Salimi-Bejestani, M. R., Mcgarry, J. W., Felstead, S., Ortiz, P., Akca, A., Williams, D. J. (2005): Development of an antibody-detection ELISA for Fasciola hepatica and its evaluation against a commercially available test. Res. Vet. Sci., 78, 177–181. DOI: 10.1016/j.rvsc.2005.08.005

    Article  CAS  PubMed  Google Scholar 

  • Sarimehmetoglu, H.O. (2002): Application of western blotting for the immunodiagnosis of Fasciola hepatica in cattle using excretory/secretory antigens. Turk. J. Vet. Anim. Sci., 26, 1061–1065

    Google Scholar 

  • Tinar, R. (2003): Life cycle. In: Tinar, R., Korkmaz, M. (Eds) Fasciolosis. İzmir, Turkey: Turkish Society for Parasitology, Publication no. 18: 13–42 (In Turkish)

    Google Scholar 

  • Torgerson, P., Claxton, J. (1999): Epidemiology and Control. In: Dalton, J. P. (Ed) Fasciolosis. Wallingford, Oxon, UK: CABI Publishing, pp. 113–149

    Google Scholar 

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Akca, A., Gokce, H.I. & Mor, N. Seroprevalence of Fasciola hepatica infection in cattle and sheep in the province of Kars, Turkey, as determined by ELISA. Helminthologia 51, 94–97 (2014). https://doi.org/10.2478/s11687-014-0215-x

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  • DOI: https://doi.org/10.2478/s11687-014-0215-x

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