Abstract
A comparative study of the effect of barley stripe mosaic virus (BSMV) and gamma irradiation on mitotic divisions in barley (Hordeum vulgare L.) roots was performed by evaluating the mitotic index (MI), micronucleus (MN) frequency and sister chromatid exchanges (SCE). Results indicate that, similarly to gamma irradiation at doses of 100, 150 and 250 Gy, BSMV reduces the mitotic activity, increases the micronucleus frequency and the rate of SCE and promotes the formation of C-metaphases. In root meristematic cells of the three barley cultivars studied (Galactic, Sonor and Unirea), the mitotic index of infected plants was found to be 52.5, 54.48 and 64.17%, respectively, lower than the uninfected control. An increase in frequency of sister chromatid exchanges was observed in all the experimental variants. In treatments involving viral infection alone or in combination with gamma irradiation chromosomes with three and more chromatid exchanges were observed, while their percentage in the control or in treatments with gamma irradiation alone was reduced. The results of the study indicate that in plants derived from irradiated seeds, BSMV produces an effect that is correlated nonlinearly with the radiation dose applied. Cytological analysis of mitotic divisions in barley roots revealed the genotoxicity of BSMV infection.
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Maluszynska J., Juchimiuk J., Plant genotoxicity: a molecular cytogenetic approach in plant bioassays, Arh. Hig. Rada Toksikol., 2005, 56, 177–184
Kozak M.F., The mitotic rhythms in representatives of soybean Glycine L., Cytologiâ i Genetika, 2004, 38, 7–12, (in Russian)
Tucker J.D., Auletta A., Cimido M.C., Dearfield K.L., Jacobson-Kram D., Tice R.R., et al., Sister chromatid exchange: second report of the Gene-Tox Program, Mutat. Res., 1993, 297, 101–180
Ivanov V.B., Cell proliferation in plants, Itogy nauki i tekhniki, Ser. Tsitologia, VINITI, Moscow, 1987, (in Russian)
Dmitrieva S.A., Minnibaeva F.V., Gordon L.Kh., Mitotic index of meristematic cells and growth of Pisum sativum roots exposed to modulators of the inositol series, Cytologiâ, 2006, 48, 475–479, (in Russian)
Singh P., Srivastava A.K., Singh A., Cell Cycle Stage Specific Application of Cypermethrin and Carbendazim to Assess the Genotoxicity in Somatic Cells of Hordeum vulgare L., Bull. Environ. Contam. Toxicol., 2008, 81, 258–261
Sandfaer J., Barley stripe mosaic virus and the frequency of triploids and aneuploids in barley, Genetics, 1973, 73, 597–603
Linde-Laursen I., Siddiqui K.A., Triploidy and aneuploidy in virus infected wheat, Triticum aestivum, Hereditas, 1974, 76, 152–154
Kovalchuk I., Kovalciuc O., Kalck V., Boyko V., Filkowski J., Heinlein M., et al., Pathogen-induced systemic plant signal triggers DNA rearrangements, Nature, 2003, 423, 760–762
Boyko A., Kathiria P., Zemp F.J., Yao Y., Pogribny I., Kovalchuk I., Transgenerational changes in the genome stability and methylation in pathogeninfected plants (Virus-induced plant genome instability), Nucl. Acids Res., 2007, 35, 1714–1725
Lucht J.M., Mauch-Mani B., Steiner H.Y., Metraux J.P., Ryals J., Hohn B., Pathogen stress increases somatic recombination frequency in Arabidopsis, Nat. Genet., 2002, 30, 311–314
Milne R.G., Lesemann D.-E., Immunosorbent electron microscopy in plant virus studies, Meth. Virol., 1984, 8, 85–101
Panda K.K., Patra J., Panda B.B., Induction of sister chromatid exchanges by heavy metal salts in root meristem cells of Allium cepa, Biol. Plantarum, 1996, 38, 555–561
Clewer A.G., Scarisbrick D.H., Practical statistics and experimental design for plant and crop science, John Wiley & Sons Ltd., Chichester, 2001
Il’inskikh N.N., Micronucleus analysis and cytogenetic instability, Tomsk University Press, 1992, (in Russian)
El-Ghamery A.A., El-Nahas A.I., Mansour M.M., The action of atrazine herbicide as an indicator of cell division on chromosomes and nucleic acids content in root meristems of A. cepa and V. faba, Cytologia, 2000, 65, 277–287
Landis W.G., Gorsuch J.W., Hughes J.S., Anthony M.L., (Eds.), Environmental toxicology and risk assessment, vol. 2, Gorsuch, Dwyer, Ingersoll, La Point, 1993
Mayhew D.E., Carroll T.W., Barley Stripe Mosaic Virions Associated with Spindle Microtubules, Science, 1974, 185, 957–958
Bozsakyová E., Wsólová L., Chalupa I., Spontaneous and gamma-ray-induced sister chromatid exchanges in patients with carcinoma of cervix uteri, Int. J. Radiat. Biol., 2005, 81, 177–185
Ucur A., Palanduz S., Cefle K., Ozturk S., Tutkan G., Vatansever S., et al., Sister chromatid exchange and mitotic index in patients with cirrhosis related to hepatitis B and C viruses and in chronic carriers, Hepato-Gastroenterology, 2003, 50, 2137–2140
Yi H., Lui J., Zheng K., Effect of sulfur dioxide hydrates on cell cycle, sister chromatid exchange, and micronuclei in barley, Ecotox. Envir. Safe., 2005, 62, 421–426
Sang N., Xin X., Municipal landfill leachate induces cytogenetic damage in root tips of Hordeum vulgare, Ecotox. Envir. Safe., 2006, 63, 469–473
Butorina A.K., Do Niû Tien, The circadian mitotic rhythms in mung bean Vigna radiata (L.) R.Wilczek, Cytologiâ, 2008, 50, 729–733, (in Russian)
Pandey R.M., Cytotoxic effects of pesticides in somatic cells of Vicia faba L., Tsitologia i Genetika, 2008, 42, 13–18
Hidalgo A., Gonzalez-Reyes J.A., Navas P., Garcia-Herdugo G., Abnormal mitosis and growth inhibition in Allium cepa root induced by propham and chloropropham, Cytobios, 1989, 57, 7–14
Sudhakar R., Ninge Gowda K.H., Venu G., Mitotic abnormalities induced by silk dyeing industry effluents in the cells of Allium cepa, Cytologia, 2001, 66, 235–239
Schneidermann M.H., Dewey W.C., Highfield D.P., Inhibition of DNA synthesis in synchronized Chinese hamster cell treated in G1 with cycloheximide, Exp. Cell Res., 1971, 67, 147–155
Kihlman B.A., Kronborg D., Sister chromatid exchange in Vicia faba. I. Demonstration by a modified fluorescent plus Giemsa (FPG) technique, Chromosoma, 1975, 51, 1–10
Perry P., Wolff S., New Giemsa method for the differential staining of sister chromatids, Nature, 1974, 251, 156–158
Wolff S., Bodycote J., Painter R.B., Sister chromatid exchanges induced in Chinese hamster cells by U.V. irradiation at different stages of cell cycle: the necessity for cell to pass through S, Mutat. Res., 1974, 25, 73–81
Ishii Y., Bender M., Effects of inhibitors of DNA synthesis on spontaneous and ultraviolet lightinduced sister-chromatid exchanges in Chinese hamster cells, Mutat. Res., 1980, 79, 19–32
Kato H., Mechanisms of sister chromatid exchanges and the relation to production of chromosomal aberrations, Chromosoma, 1977, 59, 179–191
Painter R.B., A replication model for sister-chromatid exchange, Mutat. Res., 1980, 70, 337–341
Dong Z., Fasullo M., Multiple recombination pathways for sister chromatid exchange in Saccharomyces cerevisiae: role of RAD1 and the RAD52 epistasis group genes, Nucl. Acids Res., 2003, 31, 2576–2585
Wilson D.M., Thompson L., Molecular mechanisms of sister-chromatid exchange, Mutat. Res., 2007, 616, 11–23
Andronic L., Study of the mutagenic effect of viral infection based on the analysis of sister chromatid exchanges, Proceedings of National Conference on genetics, biotechnology and crop improvement, (17–18 February, 2005, Chisinau), 2005, 17–20, (in Romanian)
Bujoreanu V., Chiriac Gh., Plant viruses as possible inducers of genotypic variability in plants, Proceedings of National Conference on genetics, biotechnology and crop improvement, (9–10 November, 1994, Chisinau), 1994, 8–10, (in Romanian)
Chiriac Gh.I., Andronic L., Bujoreanu V.V., Marii L., Features of crossing-over in virus-infected tomato, Cent. Eur. J. Biol., 2006, 1, 386–398
Mock R.J., Stokes I.E., Jullesfie A.J., Effect of sugarcane mosaic virus infection in parental stock on panicle and seed production of virus-free F2 progeny in Sorghum (Sorghum bicolor), Plant Dis., 1985, 69, 310–312
Yukhimenco A.I., Voloshchuk S.I., Girco V.S., Winter wheat viruses as biological stress factors inducing genetic variation, Proceedings of 11th congress of the federation of European societies of plant physiology, (7–11 September 1998, Varna, Bulgaria), Bulgarian J. Plant. Physiol., 1988, 222
Nemčinov L.G., Genetic variability in maize under conditions of viral pathogenesis, PhD thesis, Institute of Genetics and Cytology of the National Academy of Sciences of Belarus, Republic of Belarus, 1990, (in Russian)
Geraskin S., Oudalova A., Kim J., Dikarev V., Dikareva N., Cytogenetic effect of low dose gammaradiation in Hordeum vulgare seedlings: non-linear dose-effect relationship, Radiat. Environ. Bioph., 2007, 46, 31–41
Rogakou E.P., Boon C., Redon C., Bonner W.M., Megabase chromatin domains involved in DNA double-strand breaks in vivo, J. Cell. Biol., 1999, 146, 905–916
Liu Q., Guntuku S., Cui X.S., Matsuoka S., Cortez D., Tamai K., et al., Chk1 is an essential kinase that is regulated by Atr and required for the G(2)/M DNA damage checkpoint, Gene Dev., 2000, 14, 1448–1459
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Andronic, L.I., Jacota, A.G., Bujoreanu, V.V. et al. Genotoxicity of barley stripe mosaic virus in infected host plants. cent.eur.j.biol. 5, 633–640 (2010). https://doi.org/10.2478/s11535-010-0048-7
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DOI: https://doi.org/10.2478/s11535-010-0048-7