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Morphological and histochemical examination of male and female gonads in Homarus gammarus (L. 1758)

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Central European Journal of Biology

Abstract

The reproductive organs of both male and female European lobsters (Homarus gammarus) are H-shaped gonads that lie dorsal to the gut on the large hepatopancreas. The ovary consists of a pair of tubular, parallel lobules with a connecting bridge. The germarium of the ovary containing oogonia is concentrated in the center of the ovarian lobe. As oogonesis proceeds, the oocytes move to the peripheral regions of the ovary. The follicle cells begin to surround the oocytes in the previtellogenic stage, and the mature oocytes are completely surrounded by the follicle cells. Carbohydrates exist in both early and late vitellogenic oocytes that give PAS positive reaction. However, their rising protein content in late vitellogenic oocytes makes them stain with Bromophenol blue. Testes show convoluted lobules with a germinal epithelium and a central collecting duct, and the paired vasa deferentia have three distinct parts. Spermatophores are nonpedunculate and tubular, which extrude as a continuous column and consist of a sperm mass covered with primary and secondary layers. The primary layer stains with Bromophenol Blue and gives a PAS positive reaction. But the secondary layer only weakly stains with Bromophenol Blue. The histochemical results may indicate that the function of the two layers is different.

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References

  1. Prodöhl P.A., Jørstad K.E., Triantafyllidis A., Katsares V., Triantaphyllidis C., Evaluation of genetic impact of aquaculture activities on native populations, A European network, Genimpact, 2006

    Google Scholar 

  2. Holthius L.B., FAO Species Catalogue, Marine lobsters of the World, An annotated and illustrated catalogue of species of interest to fisheries known to date, FAO Fisheries Synopsis, 1991

    Google Scholar 

  3. Schamalenbach I., Buchholz F., Franke H.D., Saborowski R., Improvement of rearing conditions for juvenile lobsters (Homarus gammarus) by coculturing with juvenile isopods (Idotea emarginata), Aquaculture, 2009, 289, 297–303

    Article  Google Scholar 

  4. Ferguson A., Genetic diversity in the European lobster (Homarus gammarus): population structure and impacts of stock enhancement, Fisheries and Agriculture, 2002

    Google Scholar 

  5. Beal B.F., Lobster management in Maine, USA, In: Tully O. (Ed.), The Biology and Management of Clawed Lobster (Homarus gammarus L.) in Europe, Vol.2, Fisheries Resource Series, Bord lascaigh Mhara (Irish Sea Fisheries Board), Dun Lacghaire, Ireland, 2004

    Google Scholar 

  6. Beal B.F., Mercer J.P., O’Conghaile A., Survival and growth of hatchery-reared individuals of the European lobster, Homarus gammarus (L.), in field-based nursery cages on the Irish west coat, Aquaculture, 2002, 210, 137–157

    Article  Google Scholar 

  7. Schmalenbach I., Franke H.D., Potential impact of climate warming on the recruitment of an economically and ecologically important species, the European lobster (Homarus gammarus) at Helgoland, North Sea, Mar. Biol., 2010, 157, 1127–1135

    Article  Google Scholar 

  8. Schmalenbach I., Studies on the developmental conditions of the European lobster (Homarus gammarus Linnaeus, 1758) at the rocky island of Helgoland (German Bight, North Sea), PhD thesis, University of Hamburg, Hamburg, Germany, 2009

    Google Scholar 

  9. Agnalt A.L., Kristiansen T.S., Jorstad K.E., Growth, reproductive cycle, and movement of berried European lobsters (Homarus gammarus) in a local stock off southwestern Norway, J. Mar. Sci., 2006, 64, 288–297

    Google Scholar 

  10. Free E.K., Reproduction in the European Lobster (Homarus gammarus (L.). Proceedings of the Seminar at Kvitsoey 1995: the European lobster Homarus gammarus (L.), Fisk. Havet., 1998, 13, 4–26

    Google Scholar 

  11. Wenner A.M., Page H.M., Siegel P.R., Variation on size at onset of egg production, In: Wenner A.M. (Ed.), Crustacean Issues 3, Factors in Adult Growth, A.A. Balkema, Rotterdam, Boston, 1985

    Google Scholar 

  12. Pearse A.G.E., Histochemistry theoretical and applied, Vol. 2. J. and A. Churchill, London, 1960

    Google Scholar 

  13. Bancroft J.D., Stevens A., Theory and Practice of Histological Techniques, Churchill Livingstone, London, 1982

    Google Scholar 

  14. Talbot P., Helluy S., Reproduction and Embryonic Development, In: Factor J.R. (Ed.), Biology of the Lobster Homarus americanus, Academic Press Inc., San Diego California, 1995

    Google Scholar 

  15. Talbot P., The ovary of the lobster, Homarus americanus I. Architecture of the Mature Ovary, J. Ultra. Mol. Struct. Res., 1981, 76, 249–262

    Article  CAS  Google Scholar 

  16. Adiyodi R.G., Subramaniam T., Arthropoda-Crustacea, In: Adiyodi K.G., Adiyodi R.G. (Eds.), Reproductive Biology of Invertebrates I. Oogenesis oviposition and oosorption, Vol. 1, John Wiley and Sons, London, 1983

    Google Scholar 

  17. Krol R.M., Hawkins W.E., Overstreet R.M., Reproductive Components, In: Harrison F.W., Humes A. G. (Eds.), Microscopic Anatomy of Invertebrates, Decapod Crustacea, V. 10., Wiley-Liss, Inc., New York, 1992

    Google Scholar 

  18. Vogt G., Functional Anatomy, In: Holdich D.M. (Ed.), Biology of Freshwater Crayfish, Blackwell Science, USA and Canada, 2002

    Google Scholar 

  19. Hobbs H.Jr, Harvey C., Hobbs H., A comparative study of functional morphology of male reproductive system in the Astacidae with emphasis on the freshwater crayfish (Crustacea, Decapoda), Smithson Institution scholarly Press, Washington, 2007

    Google Scholar 

  20. Howard D.R., Talbot P., In vitro contraction of lobster (Homarus) ovarian muscle: methods for assaying contraction and effects of biogenic amines, J. Exp. Zool., 1992, 263, 356–366

    Article  CAS  Google Scholar 

  21. Ando H., Makioka T., Structure of the ovary and mode of oogenesis in a freshwater crayfish, Procambarus clarkii (Girard), Zool. Sci., 1998, 15, 893–901

    Article  Google Scholar 

  22. López Greco L.S., Vazquez F., Rodríguez E.M., Morphology of the male reproductive system and spermatophore formation in the freshwater ‘redclaw’ crayfish Cherax quadricarinatus (Von Martens, 1898) (Decapoda, Parastacidae), Acta Zool., 2007, 88, 223–229

    Article  Google Scholar 

  23. Unis C., Erkan M.B., Morphology and development of the female reproductive system Astacus leptodactylus (Eschscholtz, 1823) (Decapoda, Astacidae), Turkish J. Zool., 2012, 36, 775–784

    Google Scholar 

  24. Erkan M., Tunalı Y., Baş S.S., Male reproductive system morphology and spermatophore formation in Astacus leptodactylus (Eschscholtz, 1823) (Decapoda: Astacidae), J. Crustacean Biol., 2009, 29, 42–50

    Article  Google Scholar 

  25. Vazquez F.J., Tropea C., Lopez Greco L.S., Development of the female reproductive system in the freshwater crayfish Cherax quadricarinatus (Decapoda, Parastacidae), Invertebr. Biol., 2008, 127, 433–443

    Article  Google Scholar 

  26. Kessel R.G., Mechanisms of protein yolk synthesis and deposition in crustacean oocytes, Z. Zellforsch, 1968, 89, 17–38

    Article  PubMed  CAS  Google Scholar 

  27. Farmer A.S.D., Reproduction in Nephrops norvegicus (Decapoda:Nephropidae), J. Zool., 1974, 174, 161–183

    Article  Google Scholar 

  28. Schade M.L., Shivers R.R., Structural modulation of the surface and cytoplasm of oocytes during vitellogenesis in the Lobster, Homarus americanus. An electron microscope-protein tracer study, J. Morphol., 1980, 163, 13–26

    Article  Google Scholar 

  29. Beams H.W., Kessel R.G., Electron microscope studies on developing crayfish oocytes with special reference to the origin of yolk, J. Cell Biol., 1963, 18, 621–649

    Article  PubMed  CAS  Google Scholar 

  30. Abdu U., Yehezkel G., Sagi A., Oocyte development and polypeptide dynamics during ovarian maturation in the red-claw crayfish Cherax quadricarinatus, Invertebr. Reprod. Dev., 2000, 37, 75–83

    Article  Google Scholar 

  31. O’Donovan P., Abraham M., Cohen D., The ovarian cycle during the intermolt in ovigerous Macrobrachium rosenbergi, Aquaculture, 1984, 36, 347–358

    Article  Google Scholar 

  32. Roudy J.C., Amsler M.O., Ovarian development and sexual maturity staging in antarctic krill, Euphausia superba Dana (Euphausiacea), J. Crustacean Biol., 1991, 11, 236–249

    Article  Google Scholar 

  33. Kozak P., Hulak M., Policar T., Tichy F., Studies of annual gonadal development and gonadal ultrastructure in spiny-cheek crayfısh (Orconectes limosus), B. Fr. Peche Piscic., 2007, 384, 15–26

    Article  Google Scholar 

  34. Kulkarni G.K., Glade L., Fingerman M., Oogenesis and effects of neuroendocrine tissues on in vitro synthesis of protein by the ovary of the red swamp crayfish Procambarus clarkii, J. Crustacean Biol., 1991, 11, 513–522

    Article  Google Scholar 

  35. Rudolph E.H., Rojas C.S., Embryonic and early postembryonic development of the burrowing crayfish, Virilastacus araucanius (Faxon, 1914) (Decapoda, Parastacidae) under laboratory conditions, Crustaceana, 2003, 76, 835–850

    Article  Google Scholar 

  36. Beatty S.J., Morgan D.L., Gill H.S., Life history and reproductive biology of the gilgie, Cherax quinquecarinatus a freshwater crayfish endemic to southwestern Australia, J. Crustacean Biol., 2005, 25, 251–262

    Article  Google Scholar 

  37. Rotllant G., Chiva M., Durfort M., Ribes E., Internal anatomy and ultrastructure of the male reproductive system of the Norway lobster Nephrops norvegicus (Decapoda: Astacidea), J. Morph., 2012, 273, 572–585

    Article  PubMed  Google Scholar 

  38. Rudolph E., New records of intersexuality in the freshwater crayfish Samastacus spinifrons (Decapoda, Parastacidae), J. Crustacean Biol., 2002, 22, 377–389

    Google Scholar 

  39. Noro C., López Greco L.S., Buckup L., Gonad morphology and type of sexuality in Parastacus defossus Faxon 1898, a burrowing, intersexed crayfish from Southern Brazil (Decapoda: Parastacidae), Acta Zool., 2008, 89, 59–67

    Article  Google Scholar 

  40. McLaughlin P.A., Internal anatomy, In: Venberg F.J., Venberg W.B. (Eds.), The Biology of Crustacea, Vol. 5, Academic Press, New York, 1983

    Google Scholar 

  41. Kooda-Cisco M., Talbot P., Ultrastructure and role of the lobster vas deferens in spermatophore formation: the proximal segment, J. Morph., 1986, 188, 91–103

    Article  Google Scholar 

  42. Mann T., Spermatophores, Springer-Verlag, New York, 1984

    Book  Google Scholar 

  43. Aiken D.E., Waddy S.L., Mercer S.M., Confirmation of external fertilization in the American lobster, Homarus americanus, J. Crustacean Biol., 2004, 24, 474–480

    Article  Google Scholar 

  44. Adiyodi K.G., Anilkumar G., Arthropoda-Crustacea, In: Adiyodi A.G., Adiyodi K.G. (Eds.), Reproductive Biology of Invertebrates, Vol. 3 Accessory Sex Glands, Wiley & Sons, Chichester, 1988

    Google Scholar 

  45. Anilkumar G., Sudha K., Subramoniam T., Spermatophore transfer and sperm structure in the Brachyuran carb Metopograpsus messor (Decapoda: Grapsidae), J. Crustacean Biol., 1999, 19, 361–370

    Article  Google Scholar 

  46. Tudge C.C., Comparative ultrastructure of hermit crab spermatozoa (Paguroidea, Anomura, Decapoda), J. Crustacean Biol., 1992, 12, 397–409

    Article  Google Scholar 

  47. Tudge C.C., Spermatophore morphology in the hermit crab families Paguridae and Parapaguridae (Paguroidea, Anomura, Decapoda), Inverteb. Repro. Dev., 1999, 35, 203–214

    Article  Google Scholar 

  48. Kooda-Cisco M.J., Talbot P., A structural analysis of the freshly extruded spermatophore from the lobster, Homarus americanus, J. Morph., 1982, 172, 193–207

    Article  Google Scholar 

  49. Haley S.R., Spermatogenesis and spermatophore production in the Hawaiian red lobster Enoplometopus occidentalis (Randall) (Crustacea, Nephropidae), J. Morph., 1984, 180, 181–193

    Article  Google Scholar 

  50. Hinsch G.W., Walker M.H., The vas deferens of the spider crab, Libinia emarginata, J. Morph., 1974, 143, 1–19

    Article  Google Scholar 

  51. Dudenhausen E.E., Talbot P., An ultrastructural comparison of soft and hardened spermatophores from the crayfish Pacifastacus leniusculus Dana, Can. J. Zool., 1983, 61, 182–194

    Article  Google Scholar 

  52. Beach D., Talbot P., Ultrastructure comparison of sperm from the crayfishes Cherax tenuimanus and Cherax albidus, J. Crustacean Biol., 1987, 7, 205–218

    Article  Google Scholar 

  53. López Greco L.S., Lo Nostro F.L., Structural changes in the spermatophore of the freshwater ‘red claw’ crayfish Cherax quadricarinatus (Von Martens., 1898) (Decapoda, Parastacidae), Acta Zool., 2008, 89, 149–155

    Article  Google Scholar 

  54. Mazia D., Brewer P.A., Alfert M., The cytochemical staining and measurement of protein with mercúric bromophenol blue, Biol. Bull., 1953, 104, 56–67 48

    Article  Google Scholar 

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  1. Department of Biology, Faculty of Science, Istanbul University, 34134, Istanbul, Turkey

    Melike Erkan & Yasemin T. Ayun

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  1. Melike Erkan
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  2. Yasemin T. Ayun
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Correspondence to Melike Erkan.

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Erkan, M., Ayun, Y.T. Morphological and histochemical examination of male and female gonads in Homarus gammarus (L. 1758). cent.eur.j.biol. 9, 37–48 (2014). https://doi.org/10.2478/s11535-013-0148-7

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  • DOI: https://doi.org/10.2478/s11535-013-0148-7

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