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The exploration of interaction studies of smaller size, mostly ignored yet intrinsically inestimable molecules towards BSA; An example of STD and DOSY NMR

  • Research Article
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Central European Journal of Chemistry

Abstract

Larger size or novel structure molecules are always appreciated by all fields of experimental and computational science. Conversely, molecules with smaller size and simple structures are usually ignored with no explanation as to why. However, the vast majority of more diminutive molecules behave as a cornerstone in the synthesis of a bigger structural framework. Subsequently, we planned to uncover the interactions of small molecules towards macromolecules, and successfully presented the binding results of 2-aminopyridine and Isovanillin towards BSA through NMR techniques. STD epitope mapping and also the DOSY results provided evidence that Isovanillin remained closer to the binding cavity of protein. Titration experiments afforded 584 µM (0.584mM) and 487 µM (0.487 mM) dissociation constants for isovanillin and 2-aminopyridine respectively. Furthermore, changes in diffusion coefficient (with and without protein addition in DOSY spectra) were found to be 0.081 log (m2 s−1) and 0.096 log (m2 s−1) points for isovanillin and 2-aminopyridine respectively. Docking studies exhibit that these molecules can tie to site 1 (sub-area IIA) through the pi-pi interaction and hydrogen bonding with Trp213. Our results demonstrated that both compounds could be utilized as part of a transporter in the circulatory system and their extension-inspired compounds may be utilized in new drug design.

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Authors and Affiliations

  1. Laboratory of Nuclear Magnetic Resonance, Department of Chemistry, Federal University of Sao Carlos, Rodovia Washington Luiz, Km 235, Sao Carlos, Brazil

    Sheraz A. K. Tanoli, Nazish U. Tanoli & Antonio G. Ferreira

  2. Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemicals and Biological Sciences, University of Karachi, Karachi, 75210, Pakistan

    Saman Usmani &  Zaheer-Ul-Haq

Authors
  1. Sheraz A. K. Tanoli
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  2. Nazish U. Tanoli
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  3. Saman Usmani
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  4. Zaheer-Ul-Haq
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  5. Antonio G. Ferreira
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Correspondence to Sheraz A. K. Tanoli.

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Tanoli, S.A.K., Tanoli, N.U., Usmani, S. et al. The exploration of interaction studies of smaller size, mostly ignored yet intrinsically inestimable molecules towards BSA; An example of STD and DOSY NMR. cent.eur.j.chem. 12, 332–340 (2014). https://doi.org/10.2478/s11532-013-0380-7

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  • DOI: https://doi.org/10.2478/s11532-013-0380-7

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