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Direct effects of interleukin-7 on the function of human T cells in vitro

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Abstract

CD3+ T lymphocytes were isolated by positive magnetic separation from the peripheral blood of healthy donors. In the absence of any additional activating stimuli, interleukin-7 (IL-7) was shown to augment the levels of T cells expressing CD25 activation marker both in CD4-positive and in CD4-negative effector memory (CD45RA-CD197-) T cell subsets, as well as in terminally differentiated (CD45RA+CD197-) T cells, without significantly affecting the activation status of naive (CD45RA+CD197+) and central memory (CD45RA-CD197+) T cells. In addition, IL-7 noticeably enhanced the production of IL-2, interferon-γ (IFN-γ), and IL-10, but not IL-4, in T cells. The direct effects of IL-7 on T cell activation induced in vitro by MACSiBead™ particles coated with CD2, CD3, and CD28 antibodies (Abs) were also investigated. Upon cell activation, IL-7 significantly augmented the levels of CD25+ T cells in naive (CD45RA+CD197+), central memory (CD45RA-CD197+), and effector memory (CD45RA-CD197-) T-cell compartments. In addition, IL-7 facilitated activation of CD4- (but not CD4+) terminally differentiated effector (CD45RA+CD197-) T cells. Finally, IL-7 was found to upregulate the production of IL-2, IFN-γ, IL-4, and IL-10 by activated T cells. In conclusion, we speculate that IL-7 is capable of enhancing functional T cell activity without causing significant functional inbalance between various T cell subsets.

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Abbreviations

Ab:

antibody

IFN:

interferon

IL:

interleukin

PBMC:

peripheral blood mononuclear cell

Th:

helper T cell

Treg:

regulatory T cell

References

  1. Elgert K. Immunology: understanding the immune system. New Jersey: Wiley-Blackwell, 2009, 726s.

    Google Scholar 

  2. Peschon JJ, Morrissey PJ, Grabstein KH, et al. Early lymphocyte expansion is severely impaired in interleukin 7 receptor-deficient mice. J Exp Med 1994; 180: 1955.

    Article  CAS  PubMed  Google Scholar 

  3. von Freeden-Jeffry U, Vieira P, Lucian LA, McNeil T, Burdach SE, Murray R. Lymphopenia in interleukin (IL)-7 gene-deleted mice identifies IL-7 as a non-redundant cytokine. J Exp Med 1995; 181: 1519.

    Article  Google Scholar 

  4. Capitini CM, Chisti AA, Mackall CL, Modulating CL. T cell homeostasis with IL-7: preclinical and clinical. J Intern Med 2009; 266: 141.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Surh CD, Sprent J. Homeostasis of naive and memory T cells. Immunity 2008; 29: 848.

    Article  CAS  PubMed  Google Scholar 

  6. Dadi H, Ke S, Roifman CM. Activation of phosphatidylinositol-3 kinase by ligation of the interleukin-7 receptor is dependent on protein tyrosine kinase activity. Blood 1994; 84: 1579.

    CAS  PubMed  Google Scholar 

  7. Foxwell BM, Beadling C, Guschin D, Kerr I, Cantrell D. Interleukin-7 can induce the activation of Jak 1, Jak 3 and STAT 5 proteins in murine T cells. Eur J Immunol 1995; 25: 3041.

    Article  CAS  PubMed  Google Scholar 

  8. Lali FV, Crawley J, McCulloch DA, Foxwell BM. A late, prolonged activation of the phosphatidylinositol 3-kinase pathway is required for T cell proliferation. J Immunol 2004; 172: 3527.

    Article  CAS  PubMed  Google Scholar 

  9. Le Campion A, Pommier A, Delpoux A, et al. IL-2 and IL-7 determine the homeostatic balance between the regulatory and conventional CD4+ T cell compartments during peripheral T cell reconstitution. J Immunol 2012; 189: 3339.

    Article  PubMed  Google Scholar 

  10. Simonetta F, Gestermann N, Bloquet S, Bourgeois C. Interleukin-7 optimizes FOXP3+CD4+ regulatory T cells reactivity to interleukin-2 by modulating CD25 expression. PLoS One 2014; 9: e113314.

    Article  Google Scholar 

  11. Geginat J, Lanzavecchia A, Sallusto F. Proliferation and differentiation potential of human CD8+ memory T-cell subsets in response to antigen or homeostatic cytokines. Blood 2003; 101: 4260.

    Article  CAS  PubMed  Google Scholar 

  12. Todosenko NM, Shmarov VA, Malashchenko VV, et al. Erythropoietin exerts direct immunomodulatory effects on the cytokine production by activated human T-lymphocytes. Int Immunopharmacol 2016; 36: 277.

    Article  CAS  PubMed  Google Scholar 

  13. Sin JI, Kim J, Pachuk C, Weiner DB, Patchuk C. Interleukin 7 can enhance antigen-specific cytotoxic-T-lymphocyte and/or Th2-type immune responses in vivo. Clin Diagn Lab Immunol 2000; 7: 751.

    CAS  PubMed  PubMed Central  Google Scholar 

  14. Webb LM, Foxwell BM, Feldmann M. Interleukin-7 activates human naive CD4+ cells and primes for interleukin-4 production. Eur J Immunol 1997; 27: 633.

    Article  CAS  PubMed  Google Scholar 

  15. Yamaki S, Ine S, Kawabe T, et al. OX40 and IL-7 play synergistic roles in the homeostatic proliferation of effector memory CD4+ T cells. Eur J Immunol 2014; 44: 3015.

    Article  CAS  PubMed  Google Scholar 

  16. Vakkila J, Aystö S, Saarinen-Pihkala UM, Sariola H. Naive CD4+ T cells can be sensitized with IL-7. Scand J Immunol 2011; 54: 501.

    Article  Google Scholar 

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Authors and Affiliations

  1. Immanuel Kant Baltic Federal University, Kaliningrad, Russia

    Vyacheslav A. Shmarov, Vladimir V. Malashchenko, Maksim E. Meniailo, Natalia D. Gazatova, Natalia M. Todosenko, Olga B. Melashchenko, Andrei G. Goncharov & Victor I. Seledtsov

  2. Immanuel Kant Baltic Federal University, 3, Botkin street, Kaliningrad, 236016, Russia

    Victor I. Seledtsov

Authors
  1. Vyacheslav A. Shmarov
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  2. Vladimir V. Malashchenko
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  3. Maksim E. Meniailo
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  4. Natalia D. Gazatova
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  5. Natalia M. Todosenko
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  6. Olga B. Melashchenko
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  7. Andrei G. Goncharov
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  8. Victor I. Seledtsov
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Corresponding author

Correspondence to Victor I. Seledtsov.

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Shmarov, V.A., Malashchenko, V.V., Meniailo, M.E. et al. Direct effects of interleukin-7 on the function of human T cells in vitro . Eur Cytokine Netw 27, 102–107 (2016). https://doi.org/10.1684/ecn.2016.0385

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  • DOI: https://doi.org/10.1684/ecn.2016.0385

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