Abstract
Objective
To investigate the effect of tea polyphenols on cardiac function in rats with diabetic cardiomyopathy, and the mechanism by which tea polyphenols regulate autophagy in diabetic cardiomyopathy.
Methods
Sixty Sprague-Dawley (SD) rats were randomly divided into six groups: a normal control group (NC), an obesity group (OB), a diabetic cardiomyopathy group (DCM), a tea polyphenol group (TP), an obesity tea polyphenol treatment group (OB-TP), and a diabetic cardiomyopathy tea polyphenol treatment group (DCM-TP). After successful modeling, serum glucose, cholesterol, and triglyceride levels were determined; cardiac structure and function were inspected by ultrasonic cardiography; myocardial pathology was examined by staining with hematoxylin-eosin; transmission electron microscopy was used to observe the morphology and quantity of autophagosomes; and expression levels of autophagy-related proteins LC3-II, SQSTM1/p62, and Beclin-1 were determined by Western blotting.
Results
Compared to the NC group, the OB group had normal blood glucose and a high level of blood lipids; both blood glucose and lipids were increased in the DCM group; ultrasonic cardiograms showed that the fraction shortening was reduced in the DCM group. However, these were improved significantly in the DCM-TP group. Hematoxylin-eosin staining showed disordered cardiomyocytes and hypertrophy in the DCM group; however, no differences were found among the remaining groups. Transmission electron microscopy revealed that the numbers of autophagosomes in the DCM and OB-TP groups were obviously increased compared to the NC and OB groups; the number of autophagosomes in the DCM-TP group was reduced. Western blotting showed that the expression of LC3-II/I and Beclin-1 increased obviously, whereas the expression of SQSTM1/p62 was decreased in the DCM and OB-TP groups (P<0.05).
Conclusions
Tea polyphenols had an effect on diabetic cardiomyopathy in rat cardiac function and may alter the levels of autophagy to improve glucose and lipid metabolism in diabetes.
中文概要
目的
探究茶多酚对自噬的调控及对糖尿病心肌病大鼠 心功能的影响。
创新点
本研究提示了糖尿病可诱导心肌自噬水平增高, 而茶多酚可抑制这一现象;相反,高脂血症可抑 制心肌自噬,而茶多酚却能诱导被抑制的自噬。
方法
各组大鼠造模成功后测定血中的血糖、血脂水平; 心超检测大鼠心脏结构及功能变化;苏木精-伊 红(H&E)染色观察心肌结构及病理改变;透射 电镜观察自噬体的形态和数量;蛋白质印迹法 (Western blotting)检测自噬相关蛋白LC3-II、 SQSTM1/p62 及Beclin-1 的表达水平。
结论
茶多酚对糖尿病心肌病大鼠心功能具有保护作 用,机制可能通过调节自噬水平改善糖尿病心肌 糖脂代谢紊乱有关。
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Acknowledgements
We are thankful to Ms. Qi WANG and Xiao-xia LIN, the lab administrator in Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University (Hangzhou, China) for their help. Most of research was performed in the Biomedical Research Centre.
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Project supported by the Scientific and Technological Projects for Medicine and Health of Zhejiang Province (No. 2015128660) and the Major Research and Development Projects for the Zhejiang Science and Technology Agency (No. 2017C03034), China
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Zhou, H., Chen, Y., Huang, Sw. et al. Regulation of autophagy by tea polyphenols in diabetic cardiomyopathy. J. Zhejiang Univ. Sci. B 19, 333–341 (2018). https://doi.org/10.1631/jzus.B1700415
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DOI: https://doi.org/10.1631/jzus.B1700415