Abstract
Culture of Arthrospira platensis (Spirulina platensis) in human urine was investigated to get valuable biomass. NO3-N was the proper N source, in comparison with other N source, including urea, NH4-N and NO2-N. As a result, aerobic nitrification of human urine was performed, with above 93.6% nitrification percentage finally achieved with total-N (TN) load of 46.52 mg/(L·d), in which Arthrospira platensis was successfully grown. The main compositions of the obtained biomass are close to those in Zarrouk medium. Thus, it is possible to culture Arthrospira platensis in nitrified human urine for food production within bioregenerative life support systems (BLSSs).
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References
Ahn, Y.H., 2006. Sustainable nitrogen elimination biotechnologies: A review. Process Biochemistry, 41(8):1709–1721. [doi:10.1016/j.procbio.2006.03.033]
Alenka, P., Ivan, M., France, M., Eldor, A.P., James, M.T., 1998. Effects of pH and oxygen and ammonium concentrations on the community structure of nitrifying bacteria from wastewater. Applied and Environmental Microbiology, 64(10):3584–3590.
APHA, AWWA, WEF, 1995. Standard Methods for Water and Wastewater Examination. Washington DC.
Belay, A., 2002. The potential application of Spirulina (Arthrospira) as a nutritional and therapeutic supplement in health management. The Journal of the American Nutraceutical Association, 5(2):27–48.
Blüm, V., Andriske, M., Ludwig, C.H., Paaflen, U., Voeste, D., 2003. The “C.E.B.A.S. mini-module”: A self-sustaining closed aquatic ecosystem for spaceflight experimentation. Advances in Space Research, 31(1):201–210. [doi:10.1016/S0273-1177(02)00750-0]
Carvajal, N., Fernandez, M., Rodrıguez, J.P., Donoso, M., 1980. Urease of Arthrospira maxima. Phytochemistry, 21(12):2821–2823. [doi:10.1016/0031-9422(80)85048-5]
Chamorro, G., 1980. Toxicological Research on the Alga Arthrospira (in French). UNIDO, UF/MEX/78/048.
Colliver, B.B., Stephenson, T., 2000. Production of nitrogen oxide and dinitrogen oxide by autotrophic nitrifiers. Biotechnology Advances, 18(3):219–232. [doi:10.1016/S0734-9750(00)00035-5]
Costa, J.A.V., Colla, L.M., Filho, P.F.D., 2004. Improving Spirulina platensis biomass yield using a fed-batch process. Bioresource Technology, 92(3):237–241. [doi:10.1016/j.biortech.2003.09.013]
Danesi, E.D.G., Rangel-Yagui, C.O., de Carvalho, J.C.M., Sato, S., 2002. An investigation of effect of replacing nitrate by urea in the growth and production of chlorophyll by Arthrospira platensis. Biomass and Bioenergy, 23:261–269. [doi:10.1016/S0961-9534(02)00054-5]
Feng, D.L., Wu, Z.C., 2006. Culture of Arthrospira platensis in human urine for biomass production and O2 evolution. Journal of Zhejiang University SCIENCE B, 7(1):34–37. [doi:10.1631/jzus.2006.B0034]
Gòdia, F., Albiol, J., Montesinos, J.L., Pérez, J., Creus, N., Cabello, F., Mengual, X., Montras, A., Lasseur, C., 2002. MELISSA: A loop of interconnected bioreactors to develop life support in space. Journal of Biotechnology, 99(3):319–330. [doi:10.1016/S0168-1656(02)00222-5]
Gordon, R. (Ed.), 1982. Essentials of Human Physiology (2nd Ed.). Year Book Medical Publishers, Chicago.
Kowalchuk, G.A., Stephen, J.R., 2001. Ammonia-oxidizing bacteria: A model for molecular microbial ecology. Annual Review of Microbiology, 55(1):485–529. [doi:10.1146/annurev.micro.55.1.485]
Larsen, T.A., Gujer, W., 1996. Separate management of anthropogenie nutrient solutions (human urine). Water Science and Technology, 34(3–4):87–94. [doi:10.1016/0273-1223(96)00560-4]
Mota, C., Ridenoure, J., Cheng, J.Y., de los Reyes III, F.L., 2005. High levels of nitrifying bacteria in intermittently aerated reactors treating high ammonia wastewater. FEMS Microbiology Ecology, 54(3):391–400. [doi:10.1016/j.femsec.2005.05.001]
Ogbonna, J.C., Yadam, H., Tanaka, H., 1995. Light supply coefficient: A new engineering parameter for photobioreactor design. Journal of Fermentation and Bioengineering, 80(4):369–376. [doi:10.1016/0922-338X(95)94206-7]
Rangel-Yagui, C.O., Danesi, E.D.G., de Carvalho, J.C.M., Sato, S., 2004. Chlorophyll production from Spirulina platensis: cultivation with urea addition by fed-batch process. Bioresource Technology, 92(2):133–141. [doi:10.1016/j.biortech.2003.09.002]
Richmond, A., 1992. Mass Culture of Cyanobacteria. In: Mann, N.H., Carr, N.G. (Eds.), Photosynthetic Prokaryotes, 2nd Ed. Plenum Press, New York and London, p.181–210.
Rodushkin, I., Ödman, F., 2001. Application of inductively coupled mass spectrometry for elemental plasma sector field analysis of urine. Journal of Trace Elements in Medicine and Biology, 14(4):241–247. [doi:10.1016/S0946-672X(01)80010-9]
Ruiz, G., Jeison, D., Chamy, R., 2003. Nitrification with high nitrite accumulation for the treatment of wastewater with high ammonia concentration. Water Research, 37(6):1371–1377. [doi:10.1016/S0043-1354(02)00475-X]
Salisbury, F.B., Gitelson, J.I., 1997. BIOS-3: Siberian experiments in bioregenerative life support. Bioscience, 47(9):575–585. [doi:10.2307/1313164]
Soletto, D., Binaghi, L., Lodi, A., de Carvalho, J.C.M., Converti, A., 2005. Batch and fed-batch cultivations of Spirulina platensis using ammonium sulphate and urea as nitrogen sources. Aquaculture, 243(1–4):217–224. [doi:10.1016/j.aquaculture.2004.10.005]
Stein, L.Y., Arp, D.J., 1998. Loss of ammonia monooxygenase activity in Nitrosomonas europaea upon exposure to nitrite. Applied and Environmental Microbiology, 64(10):4098–4102.
Sullivan, D.M., Carpenter, D.E., 1993. Methods of Analysis for Nutrition Labeling. AOAC International, Arlington, VA, p.85–104.
Tadros, M.G., Smith, W., Mbuthia, P., Joseph, B., 1991. Manipulating Cyanobacteria: Arthrospira for Potential CELSS Diet. In: Alabama, A., Univ, M. (Eds.), NASA-HBCU Space Science and Engineering Research Forum Proceedings. USA, p.40–44.
Udert, K.M., Fux, C., Münster, M., Larsen, T.A., Siegrist, H., Gujer, W., 2003. Nitrification and autotrophic denitrification of source-separated urine. Water Science and Technology, 48:119–130.
Wheeler, R.M., Sager, J.C., Knott, W.M., Mackowiak, C.L., Stutte, G.W., Yorio, N.C., Ruffe, L.M., Peterson, B.V., Goins, G.D., Hinkle, C.R., Berry, W.L., 2003. Crop Production for Advanced Life Support Systems—Observations from the Kennedy Space Center Breadboard Project. NASA/TM-2003-211184.
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Project (No. 10376032) supported by the Natural Science Association Foundation of China (NSAF)
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Feng, Dl., Wu, Zc. & Wang, Dh. Effects of N source and nitrification pretreatment on growth of Arthrospira platensis in human urine. J. Zhejiang Univ. - Sci. A 8, 1846–1852 (2007). https://doi.org/10.1631/jzus.2007.A1846
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DOI: https://doi.org/10.1631/jzus.2007.A1846