Abstract
The 3′-terminal flanking region of porcine uncoupling protein 3 (UCP3) was cloned, the sequence data revealed 15 nucleotide substitutions among Landrace and three Chinese native pig breeds named Neijiang, Minpig and Erhualian. The continuous 9 polymorphic sites were checked by PCR-RFLP, the results indicated that Erhualian had extraordinary gene frequency, presented most significant difference by χ2 test compared with Landrace, Largewhite, Neijiang and Minpig respectively, significant level compared with Meishan; and Meishan also had significant difference compared with Landrace and Minpig respectively. These results can be concluded that Taihu pigs have special genetic characteristics among pig breeds.
Similar content being viewed by others
References
Patrick, S., Skeletal muscle uncoupling protein 3 (UCP3): mitochondrial uncoupling protein in search of a function, Curr. Opin. Clin. Nutr. Metab. Care., 2002. 5: 265.
Bell, M. E., Bhargava, A., Soriano, L. et al., Sucrose intake and corticosterone interact with cold to modulate ingestive behaviour, energy balance, autonomic outflow and neuroendocrine responses during chronic stress, J. Neuroendocrinol., 2002, 14(4): 330.
McDaneld, T. G., Nielsen, M. K., Miner, J. L., Uncoupling proteins and energy expenditure in mice divergently selected for heat loss, J. Anim. Sci., 2002, 80(3): 602.
Li, L. C., Tian, W. X., Research progress in fat acid synthase inhibitor and obsity, Chinese Science Bulletin, 2001, 46(20): 1673.
Enerback, S., Jacobsson, A., Simpson, E. M. et al., Mice lacking mitochondrial uncoupling protein are cold-sensitive but not obese, Nature, 1997, 387: 90.
Werner, P., Neuenschwander, S., Stranzinger, G., Characterization of the porcine uncoupling proteins 2 and 3 (UCP2 & UCP3) and their localization to chromosome 9 p by somatic cell hybrids, Anim. Genet., 1999, 30(3): 221.
Boss, O., Samec, S., Paoloni-Giacobino, A., Uncoupling protein-3: a new member of the mitochondrial carrier family with tissue-specific expression, FEBS Lett., 1997, 408(1): 39.
Knoll, A., Putnova, L., Dvorak, J. et al., Linkage mapping of an AvaI PCR-RFLP within the porcine uncoupling protein 3 (UCP3) gene, Anim. Genet., 2000, 31(2): 156.
Zhao, X. B., Li, N., Wu, C. X., Mitochondrial uncoupling proteins: Responsible for heat production, Bulletin of National Natural Science Foundation of China, 2000, 14(5): 261.
The Breeding Committee of the Taihu Pig, Chinese Taihu Pig, Shanghai: Shanghai Scientific and Technical Publishers, 1991, 15.
Author information
Authors and Affiliations
Corresponding author
About this article
Cite this article
Fang, M., Zhao, X., Li, N. et al. Genetic analysis on 3′- terminal flanking region of uncoupling protein 3 in different pig breeds. Chin.Sci.Bull. 47, 1541–1543 (2002). https://doi.org/10.1360/02tb9339
Received:
Issue Date:
DOI: https://doi.org/10.1360/02tb9339