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Clinical Significance of Molecular Detection of Matrix metalloproteinase-1 in Bone Marrow and Peripheral Blood in Patients with Gastric Cancer

  • Translational Research and Biomarkers
  • Published:
Annals of Surgical Oncology Aims and scope Submit manuscript

Abstract

Purpose

Matrix metalloproteinases are responsible for proteolytic degradation of basement membrane and extracellular matrix. In tumor tissues, elevated expression of matrix metalloproteinase-1 (MMP-1) has been associated with tumor invasion and metastasis. However, little is known about the expression of MMP-1 in peripheral blood (PB) and bone marrow (BM) in gastric cancer patients. Thus, the aim of the present study is to determine MMP-1 messenger RNA (mRNA) expression levels in BM and PB of patients with gastric cancer.

Methods

The study group consisted of 857 patients with gastric cancer (577 males and 280 females) ranging in age from 27 to 87 years (average 61.6 years). MMP-1 mRNA expression levels in BM and PB were evaluated quantitatively by real-time reverse-transcription polymerase chain reaction (RT-PCR).

Results

Expression of MMP-1 mRNA in BM and PB of patients with gastric cancer was significantly higher than in noncancer patients. High levels of MMP-1 mRNA expression were significantly associated with differentiated histology, tumor size, tumor invasiveness, lymph node metastasis, liver metastasis, and clinical stage. Particularly importantly, MMP-1 mRNA expression in PB was an independent factor of distant metastasis.

Conclusions

We disclosed that MMP-1 mRNA expression in peripheral blood and bone marrow of gastric cancer patients was very high, precisely reflecting staging of gastric cancer. MMP-1 mRNA expression in peripheral blood may be a useful marker for distant metastasis in gastric cancer.

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Acknowledgment

We thank Miss T. Shimooka, Mrs. K. Ogata, Miss M. Oda, Miss N. Kasagi, and Miss Y. Nakagawa for their excellent technical assistance. Grant support: CREST, Japan Science and Technology Agency (JST); Japan Society for the Promotion of Science (JSPS) Grant-in-Aid for Scientific Research, grant numbers 17109013, 17591411, 17591413, 18390367, 18590333, 18659384, and 18790964; The Ministry of Education, Culture, Sports, Science, and Technology (MEXT) Grant-in-Aid for Scientific Research on Priority Areas, grant number 18015039.

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Authors and Affiliations

  1. Department of Surgery and Molecular Oncology, Medical Institute of Bioregulation, Kyushu University, Beppu, Japan

    Yoshimasa Kosaka MD, PhD, Koshi Mimori MD, PhD, Kenji Ishikawa MD & Masaki Mori MD, PhD, FACS

  2. Gastric Surgery Division, National Cancer Center Hospital, Chuo-ku, Japan

    Takeo Fukagawa MD, PhD, Tsuyoshi Etoh MD, PhD, Hitoshi Katai MD, PhD, Takeshi Sano MD, PhD & Mitsuru Sasako MD, PhD

  3. Department of Surgery, Kitasato University School of Medicine, Sagamihara, Japan

    Yoshimasa Kosaka MD, PhD & Masahiko Watanabe MD, PhD

Authors
  1. Yoshimasa Kosaka MD, PhD
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  2. Koshi Mimori MD, PhD
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  3. Takeo Fukagawa MD, PhD
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  4. Kenji Ishikawa MD
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  5. Tsuyoshi Etoh MD, PhD
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  6. Hitoshi Katai MD, PhD
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  7. Takeshi Sano MD, PhD
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  8. Masahiko Watanabe MD, PhD
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  9. Mitsuru Sasako MD, PhD
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  10. Masaki Mori MD, PhD, FACS
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Corresponding author

Correspondence to Masaki Mori MD, PhD, FACS.

Additional information

Mitsuru Sasako and Masaki Mori contributed equally to this work.

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Kosaka, Y., Mimori, K., Fukagawa, T. et al. Clinical Significance of Molecular Detection of Matrix metalloproteinase-1 in Bone Marrow and Peripheral Blood in Patients with Gastric Cancer. Ann Surg Oncol 19 (Suppl 3), 430–437 (2012). https://doi.org/10.1245/s10434-011-1905-y

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  • DOI: https://doi.org/10.1245/s10434-011-1905-y

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