Abstract
The concentrations of copper, zinc and molybdenum were measured in liver samples from 21 normal slaughter pigs (average age about 6 months) and in 36 sows (average age about 2 years). The following mean values were found: Slaughter pigs: 15 ± 8 µg Cu/g, 45 ± 7 μg Zm/g and 1.0 ± 0.2 μg Mo/g wet weight; sows: 46 ± 70 μg Cu/g, 70 ± 26 μg Zn/g and 1.3 ± 0.3 μg Mo/g wet weight. The concentrations of all 3 elements were significantly higher in the sows than in the young pigs. There was no correlation between the concentrations of copper, zinc or molybdenum. The recorded copper levels in the slaughter pigs were in accordance with the levels of non-supplemented pigs given in the literature. The soluble hepatic copper- and zinc-binding proteins were separated into 3 different fractions by gel filtration. With increasing copper and zinc levels in the liver, a higher relative amount of these elements were found in the low molecular weight fraction.
Sammendrag
Kobber, sink og molybden er analysert i lever fra 21 slaktegriser og 36 purker slaktet i Oslo. For slaktegris, som vanligvis slaktes ved 6 måneders alder, fikk en følgende resultater: 15 ±8 μg Cu/g, 45 ± 7 μg Zn/g og 1.0 ± 0.2 μg Mo/g beregnet på våtvekt. Purker som føres til slakt i Norge har en gjennomsnittsalder på ca. 2 år og for disse fikk en følgende resultater: 46 ± 70 μg Cu/g, 70 ± 26 μg Zn/g og 1.3 ± 0.3 μg Mo/g beregnet på våtvekt. Det var signifikant forskjell på de 2 gruppene for alle 3 elementene. Kobberinnholdet i lever fra slaktegris stemte bra med angivelser for gris uten spesielt kobbertilskudd som finnes i litteraturen. Innholdet av sink og molybden stemte også bra med litteraturangivelsene. Kobbertilsetning, opptil 250 mg Cu/kg fór, nyttes som vekststimulerende fórtilsetning i mange land, men slik „high level“ kobbertilsetning til for er ikke tillatt i Norge. Ut fra dette er det overraskende med det høye kobberinnholdet i lever fra en del purker.
De løselige kobber- og sinkholdige leverproteiner ble separert i 3 distinkte fraksjoner ved gelfiltrering. Fraksjonenes omtrentlige molekylvekter var: fraksjon I > 65.000, fraksjon II 35.000 og fraksjon III 10.000. Ved økende innhold av kobber og sink i leveren var det en relativ økning av de respektive elementer i den lavmolekylære fraksjonen. Økende kobberininhold i leveren hadde derimot tilsynelatende ingen innflytelse på fordelingen av sink, slik som det tidligere er påvist hos sau.
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Acknowledgement
The authors are indepted to Milica Mjør-Grimsrud, civ.ing., for carrying out the gel filtration analyses.
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Frøslie, A., Norheim, G. The Concentrations of Copper, Zinc and Molyrdenum in Swine Liver and the Relationship to the Distrirution of Solurle Copper- and Zinc-Rinding Proteins. Acta Vet Scand 18, 471–479 (1977). https://doi.org/10.1186/BF03548410
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DOI: https://doi.org/10.1186/BF03548410