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Visualization of chromosome-specific DNA Sequences by fluorescence in situ hybridization of microdissection DNA probes with metaphase chromosomes

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Russian Journal of Genetics: Applied Research

Abstract

Currently, suppression of repetitive DNA sequences (chromosomal in situ suppression hybridization (CISS hybridization)) is used to improve the results of fluorescence in situ hybridization (FISH). However, sometimes the suppression cannot be performed due to insufficient amounts of DNA of some species. This paper presents a new approach that allows visualization of a signal from chromosome-specific DNA sequences by means of computer-assisted analysis of FISH images.

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Authors and Affiliations

  1. Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Novosibirsk, Russia

    A. G. Bogomolov, K. S. Zadesenets, T. V. Karamysheva, N. L. Podkolodnyy & N. B. Rubtsov

  2. Institute of Computational Mathematics and Mathematical Geophysics, Siberian Branch, Russian Academy of Sciences, Novosibirsk, Russia

    N. L. Podkolodnyy

  3. Novosibirsk State University, Novosibirsk, Russia

    N. B. Rubtsov

Authors
  1. A. G. Bogomolov
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  2. K. S. Zadesenets
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  3. T. V. Karamysheva
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  4. N. L. Podkolodnyy
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  5. N. B. Rubtsov
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Corresponding author

Correspondence to A. G. Bogomolov.

Additional information

Original Russian Text © A.G. Bogomolov, K.S. Zadesenets, T.V. Karamysheva, N.L. Podkolodnyy, N.B. Rubtsov, 2012, published in Vavilovskii Zhurnal Genetiki i Selektsii, 2012, Vol. 16, No. 2, pp. 348–357.

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Bogomolov, A.G., Zadesenets, K.S., Karamysheva, T.V. et al. Visualization of chromosome-specific DNA Sequences by fluorescence in situ hybridization of microdissection DNA probes with metaphase chromosomes. Russ J Genet Appl Res 2, 413–420 (2012). https://doi.org/10.1134/S2079059712060032

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  • DOI: https://doi.org/10.1134/S2079059712060032

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