Abstract
A comparative analysis of morphometric changes in lung tissue was carried out in an experiment on white rats of different postnatal age (4 weeks, 2 months, and 24 months) with a simultaneous estimation of surfactant stability during exogenous hypoxia and hyperbaric oxygenation. The morphometric indices were studied and the total morphological pattern of lung tissue was described via light microscopy and systemic quantitative analysis, and the stability of the alveolar-lining complex was estimated with the Pattl method. The studies demonstrated that a complex of structural changes develops after a hypo- and hyperoxic effect: heterogeneity and an increase in the airiness of the lung tissue, the width of the interalveolar septa, and the thickness of the bronchial and vascular walls, the degree and severity of which depends on age. An increase in surfactant stability was detected in all animals of the experimental groups, except for 2-month-old rats subjected to a hypoxic effect. A natural increase in morphometric indices was registered with age in intact animals.
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REFERENCES
Avtandilov, G.G., Osnovy kolichestvennoi patologicheskoi anatomii (Fundamentals of Quantitative Pathological Anatomy), Moscow: Meditsina, 2002.
Voinov, V.V., Efferentnaya terapiya. Membrannyi plazmoforez (Efferent Therapy. Membrane Plasmapheresis), Moscow: Novosti, 2006.
Erokhin, V.V. and Romanova, L.K., Surfactant lung system, in Kletochnaya biologiya legkikh v norme i pri patologii (Cell Biology of Healthy Lungs and in Pathology), Moscow: Meditsina, 2000.
Zapadnyuk, I.P., Zapadnyuk, V.I., and Zakhariya, E.A., Laboratornye zhivotnye: razvedenie, soderzhanie, ispol’zovanie v eksperimente (Laboratory Animals: Experimental Reproduction, Maintenance, and Use), Kiev: Vysshaya Shkola, 1983.
Kaufman, O.Ya., Orekhov, O.O., and Gusev, S.A., Pathology of the microcirculatory channels of the lungs, in Kletochnaya biologiya legkikh v norme i pri patologii (Cell Biology of Healthy Lungs and in Pathology), Moscow: Meditsina, 2000.
Kirov, M.Yu., Kuz’kov, V.V., and Nedashkovskii, V.V., Ostroe povrezhdenie legkikh pri sepsise: patogenez i intensivnaya terapiya (Acute Lung Damage Caused by Sepsis: Pathogenesis and Intensive Therapy), Arkhangelsk: Sev. Gos. Med. Univ., 2004.
Koledova, V.V., Lipid metabolism and lung resistance to edema effects, Materialy II Rossiiskogo kongressa po patofiziologii, Tezisy dokladov (Proc. II Russ. Congr. on Pathophysiology, Abstracts of Papers), Moscow: Ross. Gos. Med. Univ., 2000.
Laz’ko, A.E., Asfindiyarov, R.I., and Laz’ko, M.V., Transformations of the morphofunctional alveolus-capillary system in postnatal human ontogenesis, Morfologiya, 2001, vol. 120, no. 4, pp. 64–68.
Lakin, G.F., Biometriya (Biometry), Moscow: Vysshaya Shkola, 1990.
Levshankov, A.I. and Rozenberg, O.A., Otechestvennye preparaty legochnogo surfaktanta. Surfaktant-terapiya respiratornogo distress-sindroma novorozhdennykh i vzroslykh (National Preparations of Pulmonary Surfactant. Surfactant Therapy of Respiratory Distress Syndrome of Newborns and Adults), St. Petersburg: Ritm, 2001.
Motavkin, P.A. and Gel’tser, B.I., Klinicheskaya i eksperimental’naya patofiziologiya legkikh (Clinical and Experimental Lung Pathophysiology), Moscow: Nauka, 1998.
Nesterov, Yu.V., Alveolar surface active complex (surfactant) as a criterion for assessment of the stress sensitivity of lung tissue, Estestv. Nauki, 2007, no. 2, pp. 56–61.
Prokof’ev, V.N., Mogil’nitskaya, L.V., Morgulis, G.L., and Sherstneva, I.Ya., Biochemical composition of surfactant and its free radical processes during hyperbarooxygenation and in the posthyperoxic period, Patol. Fiziol., 1995, no. 3, pp. 40–43.
Sarkisov, D.S. and Petrov, Yu.L., Mikroskopicheskaya tekhnika (Microscopy Analysis), Moscow: Meditsina, 1996.
Todorov, I.N. and Todorov, G.I., Stress, starenie i ikh biokhimicheskaya korrektsiya (Stress, Aging, and Their Biochemical Correction), Moscow: Nauka, 2003.
Abe, S., Shiratori, S., and Takahashi, H., Respiratory tract diseases and pulmonary surfactant protein molecules, Nippon Naika Gakkai Zasshi, 2002, vol. 20, pp. 236–242.
Baritussio, A., Lung surfactant, asthma and allergens: a story in evolution, Am. J. Respir. Crit. Care Med., 2004, vol. 169, no. 5, pp. 517–523.
Boggaram, V., Regulation of surfactant protein gene expression by hyperoxia in the lung, Antioxid. Redox Signaling, 2004, vol. 6, no. 1, p. 185.
Donn, S.M. and Dalton, J., Surfactant replacement therapy in the neonate: beyond respiratory distress syndrome, Respir. Care, 2009, vol. 54, pp. 1203–1208.
Jain, M. and Sznajder, J.I., Effects of hypoxia on the alveolar epithelium, Proc. Am. Thorac. Soc., 2005, vol. 2, no. 3, pp. 202–205.
Jobe, A. and Ikegami, M., Biology of surfactant, Clin. Perinatol., 2001, vol. 28, no. 3, pp. 655–669.
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Statement on animal welfare. The work was performed in accordance with the “Rules for the Work with Experimental Animals” and the European Convention for the Protection of Experimental Animals (Directive 2010/63/EU of 22 September 2010).
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The material presented in the article is part of the experimental work carried out for 18 years within the scientific direction approved in the department and at Astrakhan State University, “Metabolic Functions of Lungs and Stress Reactivity of Lungs at Different Stages of Postnatal Ontogenesis” (director, Y.V. Nesterov).
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Translated by A. Barkhash
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Nesterov, Y.V. An Experiment on Age-Related Characteristics of the Reaction of Lung Tissue and Surfactant to Hypobaric Hypoxia and Hyperbaric Hyperoxia. Adv Gerontol 10, 249–253 (2020). https://doi.org/10.1134/S2079057020030121
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DOI: https://doi.org/10.1134/S2079057020030121