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Structural and functional characteristics of various forms of red pigment of yeast Saccharomyces cerevisiae and its synthetic analog

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Abstract

Structural and functional characteristics of the yeast red pigment (product of polymerization of N1-(β-D-ribofuranosyl)-5-aminoimidazole), isolated from ade1 mutant cells of Saccharomyces cerevisiae and its deribosylated derivatives (obtained by acid hydrolysis) and its synthetic pigment analogue (product of polymerization of N1-methyl-5-aminoimidazole in vitro) were obtained. Products of in vitro polymerization were identified using mass spectrometry. The ability of these pigments to inhibit amyloid formation using insulin fibrils was compared. All the studied compounds are able to interact with amyloids and inhibit their growth. Electron and atomic force microscopy revealed a common feature inherent in the insulin fibrils formed in the presence of these compounds—they are merged into conglomerates more stable and resistant to the effects of ultrasound than are insulin aggregates grown without pigments. We suggest that all these compounds can cause coalescence of fibrils partially blocking the loose ends and, thereby, inhibit attachment of monomers and formation of new fibrils.

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Abbreviations

AIR-N1 :

(5’-O-phospho-β-D-ribofuranosyl)-5-aminoimidazol

AIRS-N1 :

(β-D-ribofuranosyl)-5-aminoimidazol

IF:

intensity of fluorescence

P(MeAI):

polymer of N1-methyl-5-aminoimidazol

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Authors and Affiliations

  1. St. Petersburg State University, St. Petersburg, Russia

    T. R. Amen & V. V. Alenin

  2. Institute of Cytology, Russian Academy of Sciences, St. Petersburg, Russia

    E. V. Mikhailova, A. V. Artyomov, I. M. Kuznetsova, T. R. Soidla & O. V. Nevzglyadova

  3. Ioffe Physical Technical Institute, Russian Academy of Sciences, St. Petersburg, Russia

    P. A. Dementyev

  4. State Polytechnical University, St. Petersburg, Russia

    M. A. Khodorkovskii & T. O. Artamonova

Authors
  1. T. R. Amen
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  2. E. V. Mikhailova
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  3. V. V. Alenin
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  4. A. V. Artyomov
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  5. P. A. Dementyev
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  6. M. A. Khodorkovskii
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  7. T. O. Artamonova
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  8. I. M. Kuznetsova
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  9. T. R. Soidla
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  10. O. V. Nevzglyadova
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Correspondence to E. V. Mikhailova.

Additional information

Original Russian Text © T.R. Amen, E.V. Mikhailova, V.V. Alenin, A.V. Artyomov, P.A. Dementyev, M.A. Khodorkovskii, T.O. Artamonov, I.M. Kuznetsova, T.R. Soidla, O.V. Nevzglyadova, 2012, published in Tsitologiya, 2012, Vol. 54, No. 11, pp. 853–861.

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Amen, T.R., Mikhailova, E.V., Alenin, V.V. et al. Structural and functional characteristics of various forms of red pigment of yeast Saccharomyces cerevisiae and its synthetic analog. Cell Tiss. Biol. 7, 86–94 (2013). https://doi.org/10.1134/S1990519X13010021

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  • DOI: https://doi.org/10.1134/S1990519X13010021

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