Abstract
A reusable system including urease covalently bound to the surface of modified nanodiamonds (MNDs) has been developed for the multiple determination of urea. The immobilized enzyme exhibits functional activity and catalyzes the hydrolysis of urea to yield ammonia. The presence of ammonia is confirmed by the formation of a colored product after the addition of chemical reagents. It was shown that the MNDs-urease complex can function in a wide range of temperatures and pH as well as in deionized water. The complex provides a linear yield of the product at low analyte concentrations and allows the multiple determination of urea in vitro.
Similar content being viewed by others
References
Holt, K.B., Phil. Trans. Roy. Soc. A, 2007, vol. 365, pp. 2845–2861.
Schrand, A., Hens, S.A.C., and Shenderova, O.A., Crit. Rev. Solid State Mater. Sci., 2009, vol. 34, pp. 18–74.
Say, J.M., van Vreden, C., Reilly, D.J., et al., Biophys. Rev., 2011, vol. 3, pp. 171–184.
Mochalin, V.N., Shenderova, O., Ho, D., et al., Nature Nanotechnol., 2012, vol. 7, pp. 11–23.
Bondar, V.S. and Puzyr, A.P., Phys. Sol. State, 2004, vol. 46, pp. 716–719.
Puzyr, A.P., Bondar, V.S., Bukayemsky, A.A., et al., NATO Sci. Ser. II. Math. Phys. Chem., 2005, vol. 92, pp. 261–270.
Gibson, N., Shenderova, O., Luo, T.J.M., et al., Diam. Relat. Mater., 2009, vol. 18, pp. 620–626.
Mamaeva, E.S., Baron, A.V., Puzyr, A.P., Burov, A.E., and Bondar, V.S., Dokl. Biochem. Biophys., 2011, vol. 439, no. 4, pp. 182–184.
Ronzhin, N.O., Baron, A.V., Mamaeva, E.S., et al., J. Biomater. Nanobiotech., 2013, vol. 4, pp. 242–246.
Puzyr, A.P. and Bondar, V.S., RF Patent no. 2252192, Byull. Izobret., 2005, no. 14.
Yakovleva, G.E., Fermenty v klinicheskoi biokhimii (posobie dlya vrachei) (Enzymes in Clinical Biochemistry (Manual for Physicians)), Novosibirsk Vektor-Best, 2005.
Brandt, J., Andersson, L.O., and Porath, J., Biochim. Biophys. Acta, 1975, vol. 386, pp. 196–202.
Mateescu, M.-A., Weltrowska, G., Agostinelli, E., et al., Biotechnol. Tech., 1989, vol. 3, pp. 415–420.
Osterman, L.A., Khromatografiya belkov i nukleinovykh kislot (Chromatography of Proteins and Nucleic Acids), Moscow Nauka, 1985.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © N.O. Ronzhin, A.V. Baron, V.S. Bondar, I.I. Gitelson, 2015, published in Doklady Akademii Nauk, 2015, Vol. 465, No. 6, pp. 741-744.
Rights and permissions
About this article
Cite this article
Ronzhin, N.O., Baron, A.V., Bondar, V.S. et al. Designing a reusable system based on nanodiamonds for biochemical determination of urea. Dokl Biochem Biophys 465, 428–431 (2015). https://doi.org/10.1134/S1607672915060216
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1607672915060216