Abstract
A technique for detection of staphylococcal enterotoxin A with sandwich enzyme-linked immunoassay (ELISA) was developed. Mouse monoclonal anti-SEA antibodies were used as capture antibodies and phage displayed anti-SEA scFv were used as detection antibodies. The limit of detection was 6–12.5 ng/mL for different pairs of antibodies. Some conditions of phage-displayed antibodies for storage in dissolved and lyophilized state were examined. It was shown the use of trehalose and arginine preserved the functional activity of phage-displayed antibodies.
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Abbreviations
- BSA:
-
bovine serum albumin
- PBS:
-
phosphate buffered saline
- PBST:
-
PBS with 0.1% Tween 20
- PBST-M:
-
PBST with 2% milk powder
- scFv:
-
single chain variable fragment mini-antibody
- SEA and SEE:
-
Staphylococcal enterotoxins A and E
- ELISA:
-
enzyme immunoassay
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Original Russian Text © K.K. Fursova, M.P. Shchannikova, A.O. Shepelyakovskaya, L.L. Pavlik, F.A. Brovko, 2016, published in Bioorganicheskaya Khimiya, 2016, Vol. 42, No. 3, pp. 327–333.
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Fursova, K.K., Shchannikova, M.P., Shepelyakovskaya, A.O. et al. Staphylococcal enterotoxin a detection with phage displayed antibodies. Russ J Bioorg Chem 42, 294–299 (2016). https://doi.org/10.1134/S1068162016020035
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DOI: https://doi.org/10.1134/S1068162016020035