Abstract
The prion protein is considered as one of the membrane targets of the neurotoxic beta-amyloid during development of Alzheimer’s disease. We chose and synthesized peptide fragments that corresponded to the 17–33, 23–33, 95–110, and 101–115 sequences of the prion protein and are responsible for the betaamyloid binding. The effect of immunization with the peptides on the development of symptoms of Alzheimer’s disease was investigated on animals with an experimentally induced form of the disease. Immunization with either free 17–33 peptide or with protein conjugates of the 23–33 and 101–115 peptides was shown to restore spatial memory of the animals. Immunization with the 17–33 peptide was also shown to decrease the level of brain beta-amyloid and to recover morphofunctional parameters of the brain.
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Abbreviations
- BE:
-
subjected to a bulbectomy
- SO:
-
sham operated
- CFA:
-
complete Freund’s adjuvant
- Fmoc:
-
9-fluorenylmethoxycarbonyl
- IFA:
-
incomplete Freund’s adjuvant
- KLH:
-
keyhole limpet hemocyanin
- OVA:
-
ovalbumin
- PBS:
-
phosphate buffered saline
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Original Russian Text © O.M. Volpina, T.D. Volkova, N.I. Medvinskaya, A.V. Kamynina, Ya.V. Zaporozhskaya, I.Yu. Aleksandrova, D.O. Koroev, A.N. Samokhin, I.V. Nesterova, V.I. Deygin, N.V. Bobkova, 2015, published in Bioorganicheskaya Khimiya, 2015, Vol. 41, No. 2, pp. 145–153.
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Volpina, O.M., Volkova, T.D., Medvinskaya, N.I. et al. Protective activity of fragments of the prion protein after immunization of animals with experimentally induced Alzheimer’s disease. Russ J Bioorg Chem 41, 125–132 (2015). https://doi.org/10.1134/S1068162015020168
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DOI: https://doi.org/10.1134/S1068162015020168