Skip to main content
SpringerLink
Log in

Enzymatic activity of thymidine kinases of a herpes simplex virus strains resistant to acyclovir H-phosphonates

  • Published:
Russian Journal of Bioorganic Chemistry Aims and scope Submit manuscript

Abstract

Some cloned laboratory mutant strains of herpes simplex virus type I resistant to acyclovir H-phosphonate were studied. As was shown for all the clones, the mutations augmented the susceptibility to cidîfovir. Thymidine kinase of the mutant viruses partially retained the capacity to phosphorylate thymidine, but lost the capacity to phosphorylate BVDU.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

ACV:

acyclovir or (9-[(hydroxyethyl)methyl]guanine

BVDU:

E-5-(2-bromovinyl)-2′-deoxyuridine

Hp-ACV:

acyclovir H-phosphonate

CDV:

cidofovir

HSV-1:

herpes simplex virus type 1

PFU:

plaque-forming unit

References

  1. Elion, G.B., J. Med. Virol., 1993, Suppl. 1, pp. 2–6.

  2. Morfin, F. and Thouvenot, D., J. Clin. Virol., 2003, vol. 26, pp. 29–37.

    Article  PubMed  CAS  Google Scholar 

  3. Hill, E.L., Hunter, G.A., and Ellis, M.N., Antimicrob. Agents Chemother., 1991, vol. 35, pp. 2322–2328.

    PubMed  CAS  Google Scholar 

  4. Karpenko, I.L., Jasko, M.V., Andronova, V.L., Ivanov, A.V., Kukhanova, M.K., Galegov, G.A., and Skoblov, Y.S., Nucleosides Nucleotides Nucleic Acids, 2003, vol. 22, pp. 319–328.

    Article  PubMed  CAS  Google Scholar 

  5. Ias’ko, M.V., Ulanova, N.Yu., Andronova, V.L., Ivanov, A.V., Karpenko, I.L., Kukhanova, M.K., Galegov, G.A., and Skoblov, Yu.S., Russ. J. Bioorg. Chem., 2004, vol. 30, pp. 539–546.

    Article  Google Scholar 

  6. Skoblov, Y.S., Karpenko, I.L., Jasko, M.V., Kukhanova, M.K., Andronova, V.L., Galegov, G.A., Sidorov, G.V., and Myasoedov, N.F., Chem. Biol. Drug. Des., 2007, vol. 69, pp. 429–434.

    Article  PubMed  CAS  Google Scholar 

  7. Gus’kova, A.A., Skoblov, M.Y., Korovina, A.N., Yasko, M.V., Karpenko, I.L., Kukhanova, M.K., Andronova, V.L., Galegov, G.A., and Skoblov, Y.S., Chem. Biol. Drug. Des., 2009, vol. 74, pp. 382–389.

    Article  PubMed  Google Scholar 

  8. Naesens, L. and De Clercq, E., Herpes, 2001, vol. 8, pp. 12–16.

    PubMed  CAS  Google Scholar 

  9. Suzutani, T., Saijo, M., Nagamine, M., Ogasawara, M., and Azuma, M., J. Clin. Microbiol., 2000, vol. 38, pp. 1839–1844.

    PubMed  CAS  Google Scholar 

  10. Saijo, M., Suzutani, T., De Clercq, E., Niikura, M., Maeda, A., Morikawa, S., and Kurane, I., Antiviral. Res., 2002, vol. 56, pp. 253–262.

    Article  PubMed  CAS  Google Scholar 

  11. L’vov, N.D., Andronova, V.L., Leont’eva, N.A., and Galegov, G.A., Vopr. Virusol., 1999, vol. 44, pp. 247–249.

    PubMed  Google Scholar 

  12. De Clercq, E., Descamps, J., Verhelst, G., Walker, R.T., Jones, A.S., Torrence, P.F., and Shugar, D., J. Infect. Dis., 1980, vol. 141, pp. 563–574.

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Research Center for Medical Genetics, Russian Academy of Medical Sciences, Moscow, Russia

    A. A. Gus’kova & M. Yu. Skoblov

  2. Ivanovskii Institute of Virology, Russian Academy of Medical Sciences, Moscow, Russia

    V. L. Andronova & G. A. Galegov

  3. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia

    S. N. Kochetkov

  4. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997, Russia

    Yu. S. Skoblov

Authors
  1. A. A. Gus’kova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Yu. Skoblov
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. V. L. Andronova
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. G. A. Galegov
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. S. N. Kochetkov
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Yu. S. Skoblov
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yu. S. Skoblov.

Additional information

Original Russian Text © A.A. Gus’kova, M.Yu. Skoblov, V.L. Andronova, G.A. Galegov, S.N. Kochetkov, Yu.S. Skoblov, 2011, published in Bioorganicheskaya Khimiya, 2011, Vol. 37, No. 5, pp. 627–630.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gus’kova, A.A., Skoblov, M.Y., Andronova, V.L. et al. Enzymatic activity of thymidine kinases of a herpes simplex virus strains resistant to acyclovir H-phosphonates. Russ J Bioorg Chem 37, 560–563 (2011). https://doi.org/10.1134/S1068162011050074

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1068162011050074

Keywords

Search

Navigation