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New polypeptide components from the Heteractis crispa sea anemone with analgesic activity

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Abstract

Two new polypeptide components which exhibited an analgesic effect in experiments on mice were isolated from the Heteractis crispa sea tropical anemone by the combination of chromatographic methods. The APHC2 and APHC3 new polypeptides consisted of 56 amino acid residues and contained six cysteine residues. Their complete amino acid sequence was determined by the methods of Edman sequencing, mass spectrometry, and peptide mapping. An analysis of the primary structure of the new peptides allowed for their attribution to a large group of trypsin inhibitors of the Kunitz type.

An interesting biological function of the new polypeptides was their analgesic effect on mammals, which is possibly realized via the modulation of the activity of the TRPV1 receptor and was not associated with the residual inhibiting activity towards trypsin and chymotrypsin. The analgesic activity of the APHC3 polypeptide was measured on the hot plate model of acute pain and was significantly higher than that of APHC2. Methods of preparation of the recombinant analogues were created for both polypeptides.

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Abbreviations

ASIC:

proton-sensitive channel

BPTI:

bovine pancreatic trypsin inhibitor

EDTA:

ethylenediaminetetraacetic acid

IPTG:

isopropyl-β-D-1-thiogalatopyranoside

TFA:

trifluoroacetic acid

TRPV1:

vanilloid receptor 1

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Authors and Affiliations

  1. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997, Russia

    S. A. Kozlov, Ya A. Andreev & E. V. Grishin

  2. Branch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Pushchino Division Russian Academy of Sciences, Pushchino, Moscow oblast, 142292, Russia

    A. N. Murashev, D. I. Skobtsov & I. A. D’yachenko

Authors
  1. S. A. Kozlov
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  2. Ya A. Andreev
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  3. A. N. Murashev
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  4. D. I. Skobtsov
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  5. I. A. D’yachenko
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  6. E. V. Grishin
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Correspondence to S. A. Kozlov.

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Original Russian Text © S.A. Kozlov, Ya,A. Andreev, A.N. Murashev, D.I. Skobtsov, I.A. D’yachenko, E.V. Grishin 2009, published in Bioorganicheskaya Khimiya, 2009, Vol. 35, No. 6, pp. 789–498.

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Kozlov, S.A., Andreev, Y.A., Murashev, A.N. et al. New polypeptide components from the Heteractis crispa sea anemone with analgesic activity. Russ J Bioorg Chem 35, 711–719 (2009). https://doi.org/10.1134/S1068162009060065

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  • DOI: https://doi.org/10.1134/S1068162009060065

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