Abstract
It is common knowledge that zebrafish, Danio rerio, oocytes in their follicular envelope that have reached definitive size undergo in vitro maturation in 90% Leibovitz’s medium, pH 9.0, when treated with 17α, 20β-dihydroxyprogesterone and acquire developmental competence but do not ovulate (Seki et al., 2008). We have demonstrated that zebrafish oocytes that have undergone maturation under the indicated conditions ovulate when treated with prostaglandin F2α (5 μg/mL) and/or 20% carp ovarial fluid and are capable of development towards the actively feeding larvae upon fertilization (the maximum follow-up period).
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Abe, T., Ijiri, S., Adachi, S., and Yamauchi, K., Development of an in vitro culture system for producing eel larvae from immature ovarian follicles in Japanese eel Anguilla japonica, Fish Sci., 2010, vol. 76, pp. 257–265.
Agresti, A. and Coull, B.A., Approximate is better than “exact” in interval estimation of binominal proportions, Am. Stat., 1998, vol. 52, pp. 119–126.
Bouffard, R.E., The role of prostaglandins during sexual maturation, ovulation and spermiation in the goldfish, Carassius auratus, M. Sc. Thesis, Vacouver: University of British Columbia, 1979.
Cetta, E. and Goetz, F.W., Ovarian and plasma prostaglandin E and F levels in brook trout (Salvelinus fontinalis) during pituitary-induced ovulation, Biol. Reprod., 1982, vol. 27, pp. 1216–1221.
Epler, P., Effect of steroid and gonadotropic hormones on the maturation of carp ovaries. Part V. Ovulation, fertilization and embryonic development of carp oocytes in vitro, Pol. Arch. Hydrobiol., 1981, vol. 28, no. 1, pp. 119–125.
Fujimori, C., Ogiwara, K., Hagiwara, A., et al., Expression of cyclooxygenase-2 and prostaglandin receptor EP4b mRNA in the ovary of the medaka fish, Oryzias latipes: possible involvement in ovulation, Mol. Cell Endocrinol., 2011, vol. 332, nos. 1–2, pp. 67–77.
Fujimori, C., Ogiwara, K., Hagiwara, A., and Takahashi, T., New evidence for the involvement of prostaglandin receptor ep4b in ovulation of the medaka, Oryzias latipes, Mol. Cell Endocrinol., 2012, vol. 362, nos. 1–2, pp. 76–84.
Goetz, F.W. and Theofan, G., In vitro stimulation of germinal vesicle breakdown and ovulation of yellow perch (Perca flavescens) oocytes. Effects of 17 alpha-hydroxy-20 beta-dihydroprogesterone and prostaglandins, Gen. Comp. Endocrinol., 1979, vol. 37, no. 3, pp. 273–285.
Goetz, F.W., Smith, D.C., and Krickl, S.P., The effects of prostaglandins, phosphodiesterase inhibitors, and cyclic amp on ovulation of brook trout (Salvelinus fontinalis) oocytes, Gen. Comp. Endocrinol., 1982, vol. 48, no. 2, pp. 154–160.
Hagiwara, A., Ogiwara, K., Katsu, Y., and Takahashi, T., Luteinizing hormone-induced expression of Ptger4b, a prostaglandin E2 receptor indispensable for ovulation of the medaka Oryzias latipes, is regulated by a genomic mechanism involving nuclear progestin receptor, Biol. Reprod., 2014, vol. 90, no. 6, pp. 1–14.
Jalabert, B. and Szollosi, D., In vitro ovulation of trout oocytes: effect of prostaglandin on smooth muscle-like cells of the theca, Prostaglandins, 1975, vol. 9, pp. 765–778.
Jalabert, B., In vitro oocyte maturation and ovulation in rainbow trout (salmo gardneri), northern pike (exos luxius), and goldfish (carassius auratus), J. Fish. Res, 1976, vol. 33, pp. 974–988.
Knight, O.M., Characterizing the role of eicosanoids in maturation-inducing steroid-mediated ovulation and spawning in the zebrafish (Danio rerio), M. Sc. Thesis, Ontario: University of Guelph, 2014.
Li, S., Mao, Z., Han, W., et al., In vitro oocyte maturation in the zebra fish, Brachydanio rerio, and the fertilization and development of the mature egg, Chin. J. Biotechnol., 1993, vol. 9, no. 4, pp. 247–255.
Lister, A.L. and Van Der Kraak, G., An investigation into the role of prostaglandins in zebrafish oocyte maturation and ovulation, Gen. Comp. Endocrinol., 2008, vol. 159, no. 1, pp. 46–57.
Lister, A.L. and Van Der Kraak, G.J., Regulation of prostaglandin synthesis in ovaries of sexually-mature zebrafish (Danio rerio), Mol. Reprod. Develop., 2009, vol. 76, no. 11, pp. 1064–1075.
Melnyk, N.C., Regulation of prostaglandin synthesis in the zebrafish ovary, M. Sc. Thesis, Ontario: University of Guelp, 2011.
Nagahama, Y. and Yamashita, M., Regulation of oocyte maturation in fish, Dev. Growth Differ., 2008, vol. 50, suppl. 1, pp. S195–S219.
Ogiwara, K., Takano, N., Shinohara, M., et al., Gelatinase A and membrane-type matrix metalloproteinases 1 and 2 are responsible for follicle rupture during ovulation in the medaka, Proc. Natl. Acad. Sci. U. S. A., 2005, vol. 102, no. 24, pp. 8442–8447.
Ogiwara, K., Fujimori, C., Rajapakse, S., and Takahashi, T., Characterization of luteinizing hormone and luteinizing hormone receptor and their indispensable role in the ovulatory process of the medaka, PLoS One, 2013, vol. 8, no. 1, p. e54482.
Saat, T.V., Maturation and ovulation of carp oocytes in different media, Uch. Zap. Tartu. Gos. Univ., 1988, no. 805, pp. 5–34.
Seki, S., Kouya, T., Tsuchiya, R., et al., Development of a reliable in vitro maturation system for zebrafish oocytes, Reproduction, 2008, vol. 135, pp. 285–292.
Selman, K., Petrino, T.R., and Wallace, R.A., Experimental conditions for oocyte maturation in the zebrafish, Brachydanio rerio, J. Exp. Zool., 1994, vol. 269, pp. 538–550.
Skoblina, M.N., In vitro stimulation of oocyte ovulation in teleosts by gonadotropic and steroid hormones, Russ. J. Dev. Biol., 2009, vol. 40, no. 4, pp. 191–197.
Skoblina, M.N. and Minin, A.A., Hormonal induction of in vitro maturation and ovulation of loach oocytes and obtaining egg cells capable of fertilization and development, Russ. J. Dev. Biol., 2015, vol. 46, no. 3, pp. 159–168.
Stacey, N.E. and Pandy, S., Effects of indomethacin and prostaglandins on ovulation of goldfish, Prostaglandins, 1975, vol. 9, pp. 597–607.
Westerfield, M., The Zebrafish Book. A Guide for the Laboratory Use of Zebrafish (Danio rerio), 4th ed. (available online), Eugene: University of Oregon Press, 2000.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © M.N. Skoblina, A.A. Minin, 2016, published in Ontogenez, 2016, Vol. 47, No. 5, pp. 314–319.
Rights and permissions
About this article
Cite this article
Skoblina, M.N., Minin, A.A. Hormone-induced in vitro maturation and ovulation of Danio rerio oocytes and production of eggs capable of fertilization and futher development. Russ J Dev Biol 47, 294–299 (2016). https://doi.org/10.1134/S106236041605009X
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S106236041605009X